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Adjustment of sensitisation and challenge protocols restores functional and inflammatory responses to ovalbumin in guinea-pigs.

Lowe AP, Broadley KJ, Nials AT, Ford WR, Kidd EJ - J Pharmacol Toxicol Methods (2014)

Bottom Line: Increasing the number of sensitisation injections from 2 to 3 did not alter the responses.Increasing the Ova sensitisation concentration from 100 to 150 μg significantly increased total cells, particularly eosinophils.It has also demonstrated an important dissociation between EAR, LAR, AHR and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.

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The total cell (A), macrophage (B), eosinophil (C), lymphocyte (D) and neutrophil (E) counts in bronchoalveolar fluid in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged with on day 15 with saline (sal), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 3), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). N = 6; * significantly different from the previous protocol (protocol 4 in the case of protocol 6) p < 0.05, **p < 0.01, ***p < 0.001; performed with a two tailed Student's t-test.
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f0015: The total cell (A), macrophage (B), eosinophil (C), lymphocyte (D) and neutrophil (E) counts in bronchoalveolar fluid in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged with on day 15 with saline (sal), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 3), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). N = 6; * significantly different from the previous protocol (protocol 4 in the case of protocol 6) p < 0.05, **p < 0.01, ***p < 0.001; performed with a two tailed Student's t-test.

Mentions: 100 μg/ml Ova challenge significantly increased total lavage cells (protocol 1, Fig. 3A, 3.2 ± 0.5 × 106/ml) compared to saline (1.6 ± 0.13 × 106/ml). Eosinophils (Fig. 3C) made up most of this increase (1.3 ± 0.3 × 106/ml) compared to saline (0.05 ± 0.01 × 106/ml). Increasing the Ova challenge concentration (protocol 2) significantly increased the total cell numbers (5.3 ± 0.4 × 106/ml) compared to protocol 1 (3.2 ± 0.5 × 106/ml). Eosinophils were significantly elevated (2.0 ± 0.2 × 106/ml) compared to protocol 1 (1.3 ± 0.3 × 106/ml). Increasing the number of 100 μg Ova sensitisation injections (protocol 3) had no effect on any cell type measured.


Adjustment of sensitisation and challenge protocols restores functional and inflammatory responses to ovalbumin in guinea-pigs.

Lowe AP, Broadley KJ, Nials AT, Ford WR, Kidd EJ - J Pharmacol Toxicol Methods (2014)

The total cell (A), macrophage (B), eosinophil (C), lymphocyte (D) and neutrophil (E) counts in bronchoalveolar fluid in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged with on day 15 with saline (sal), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 3), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). N = 6; * significantly different from the previous protocol (protocol 4 in the case of protocol 6) p < 0.05, **p < 0.01, ***p < 0.001; performed with a two tailed Student's t-test.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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f0015: The total cell (A), macrophage (B), eosinophil (C), lymphocyte (D) and neutrophil (E) counts in bronchoalveolar fluid in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged with on day 15 with saline (sal), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 3), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). N = 6; * significantly different from the previous protocol (protocol 4 in the case of protocol 6) p < 0.05, **p < 0.01, ***p < 0.001; performed with a two tailed Student's t-test.
Mentions: 100 μg/ml Ova challenge significantly increased total lavage cells (protocol 1, Fig. 3A, 3.2 ± 0.5 × 106/ml) compared to saline (1.6 ± 0.13 × 106/ml). Eosinophils (Fig. 3C) made up most of this increase (1.3 ± 0.3 × 106/ml) compared to saline (0.05 ± 0.01 × 106/ml). Increasing the Ova challenge concentration (protocol 2) significantly increased the total cell numbers (5.3 ± 0.4 × 106/ml) compared to protocol 1 (3.2 ± 0.5 × 106/ml). Eosinophils were significantly elevated (2.0 ± 0.2 × 106/ml) compared to protocol 1 (1.3 ± 0.3 × 106/ml). Increasing the number of 100 μg Ova sensitisation injections (protocol 3) had no effect on any cell type measured.

Bottom Line: Increasing the number of sensitisation injections from 2 to 3 did not alter the responses.Increasing the Ova sensitisation concentration from 100 to 150 μg significantly increased total cells, particularly eosinophils.It has also demonstrated an important dissociation between EAR, LAR, AHR and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.

Show MeSH
Related in: MedlinePlus