Adjustment of sensitisation and challenge protocols restores functional and inflammatory responses to ovalbumin in guinea-pigs.
Bottom Line: Increasing the number of sensitisation injections from 2 to 3 did not alter the responses.Increasing the Ova sensitisation concentration from 100 to 150 μg significantly increased total cells, particularly eosinophils.It has also demonstrated an important dissociation between EAR, LAR, AHR and inflammation.
Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.Show MeSH
Related in: MedlinePlus
Mentions: 100 μg/ml Ova challenge significantly increased total lavage cells (protocol 1, Fig. 3A, 3.2 ± 0.5 × 106/ml) compared to saline (1.6 ± 0.13 × 106/ml). Eosinophils (Fig. 3C) made up most of this increase (1.3 ± 0.3 × 106/ml) compared to saline (0.05 ± 0.01 × 106/ml). Increasing the Ova challenge concentration (protocol 2) significantly increased the total cell numbers (5.3 ± 0.4 × 106/ml) compared to protocol 1 (3.2 ± 0.5 × 106/ml). Eosinophils were significantly elevated (2.0 ± 0.2 × 106/ml) compared to protocol 1 (1.3 ± 0.3 × 106/ml). Increasing the number of 100 μg Ova sensitisation injections (protocol 3) had no effect on any cell type measured.
Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.