Adjustment of sensitisation and challenge protocols restores functional and inflammatory responses to ovalbumin in guinea-pigs.
Bottom Line: Lack of AHR and LAR with the original protocol was confirmed.Increasing the Ova challenge concentration from 100 to 300 μg/ml restored AHR and eosinophils and increased the peak of the EAR.It has also demonstrated an important dissociation between EAR, LAR, AHR and inflammation.
Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.Show MeSH
Related in: MedlinePlus
Mentions: 100 μg/ml Ova challenge significantly increased total lavage cells (protocol 1, Fig. 3A, 3.2 ± 0.5 × 106/ml) compared to saline (1.6 ± 0.13 × 106/ml). Eosinophils (Fig. 3C) made up most of this increase (1.3 ± 0.3 × 106/ml) compared to saline (0.05 ± 0.01 × 106/ml). Increasing the Ova challenge concentration (protocol 2) significantly increased the total cell numbers (5.3 ± 0.4 × 106/ml) compared to protocol 1 (3.2 ± 0.5 × 106/ml). Eosinophils were significantly elevated (2.0 ± 0.2 × 106/ml) compared to protocol 1 (1.3 ± 0.3 × 106/ml). Increasing the number of 100 μg Ova sensitisation injections (protocol 3) had no effect on any cell type measured.
Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.