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Adjustment of sensitisation and challenge protocols restores functional and inflammatory responses to ovalbumin in guinea-pigs.

Lowe AP, Broadley KJ, Nials AT, Ford WR, Kidd EJ - J Pharmacol Toxicol Methods (2014)

Bottom Line: Lack of AHR and LAR with the original protocol was confirmed.Increasing the Ova challenge concentration from 100 to 300 μg/ml restored AHR and eosinophils and increased the peak of the EAR.It has also demonstrated an important dissociation between EAR, LAR, AHR and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.

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Related in: MedlinePlus

The mean time-courses for sGaw values in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with saline (saline), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). The histogram represents the maximum bronchoconstriction values recorded during the early asthmatic response (EAR) (0 –6 h) and late asthmatic response (LAR) (6–12 h). Mean changes in sGaw are expressed as mean ± SEM percentage change from baseline. A negative value represents a bronchoconstriction. N = 6; * significantly different from paired protocol p < 0.05, **p < 0.01, ***p < 0.001 performed with a two tailed Student's t-test.
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f0005: The mean time-courses for sGaw values in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with saline (saline), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). The histogram represents the maximum bronchoconstriction values recorded during the early asthmatic response (EAR) (0 –6 h) and late asthmatic response (LAR) (6–12 h). Mean changes in sGaw are expressed as mean ± SEM percentage change from baseline. A negative value represents a bronchoconstriction. N = 6; * significantly different from paired protocol p < 0.05, **p < 0.01, ***p < 0.001 performed with a two tailed Student's t-test.

Mentions: Fig. 1 represents the mean time-course changes in sGaw over 24 h following Ova challenge in conscious guinea-pigs sensitised and challenged with saline or protocols 1–6.


Adjustment of sensitisation and challenge protocols restores functional and inflammatory responses to ovalbumin in guinea-pigs.

Lowe AP, Broadley KJ, Nials AT, Ford WR, Kidd EJ - J Pharmacol Toxicol Methods (2014)

The mean time-courses for sGaw values in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with saline (saline), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). The histogram represents the maximum bronchoconstriction values recorded during the early asthmatic response (EAR) (0 –6 h) and late asthmatic response (LAR) (6–12 h). Mean changes in sGaw are expressed as mean ± SEM percentage change from baseline. A negative value represents a bronchoconstriction. N = 6; * significantly different from paired protocol p < 0.05, **p < 0.01, ***p < 0.001 performed with a two tailed Student's t-test.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4370377&req=5

f0005: The mean time-courses for sGaw values in guinea-pigs sensitised with 2 injections of 100 μg/ml ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with saline (saline), 100 μg/ml (protocol 1), 300 μg/ml Ova (protocol 2), sensitised with 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 and challenged on day 15 with 300 μg/ml Ova (protocol 4), 3 injections of 150 μg ovalbumin (Ova) and 150 mg Al(OH)3 and challenged on day 15 with 300 μg/ml (protocol 5) or 3 injections of 150 μg ovalbumin (Ova) and 100 mg Al(OH)3 on day 21 (protocol 6). The histogram represents the maximum bronchoconstriction values recorded during the early asthmatic response (EAR) (0 –6 h) and late asthmatic response (LAR) (6–12 h). Mean changes in sGaw are expressed as mean ± SEM percentage change from baseline. A negative value represents a bronchoconstriction. N = 6; * significantly different from paired protocol p < 0.05, **p < 0.01, ***p < 0.001 performed with a two tailed Student's t-test.
Mentions: Fig. 1 represents the mean time-course changes in sGaw over 24 h following Ova challenge in conscious guinea-pigs sensitised and challenged with saline or protocols 1–6.

Bottom Line: Lack of AHR and LAR with the original protocol was confirmed.Increasing the Ova challenge concentration from 100 to 300 μg/ml restored AHR and eosinophils and increased the peak of the EAR.It has also demonstrated an important dissociation between EAR, LAR, AHR and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Cardiff School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3NB, United Kingdom.

Show MeSH
Related in: MedlinePlus