Limits...
Adolescent intermittent ethanol exposure is associated with increased risky choice and decreased dopaminergic and cholinergic neuron markers in adult rats.

Boutros N, Semenova S, Liu W, Crews FT, Markou A - Int. J. Neuropsychopharmacol. (2014)

Bottom Line: Binge drinking is prevalent during adolescence and may have effects on the adult brain and behavior.Risky choice was negatively correlated with choline acetyltransferase, implicating decreased forebrain cholinergic activity in risky choice.The decreases in tyrosine hydroxylase and choline acetyltransferase immunoreactivity suggest that adolescent intermittent ethanol exposure has enduring neural effects that may lead to altered adult behaviors, such as increased risky decision making.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychiatry, University of California San Diego, La Jolla, California (Drs Boutros, Semenova, Markou); Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina (Drs Liu, Crews).

Show MeSH

Related in: MedlinePlus

Choline acetyltransferase-positive immunoreactivity (ChAT+IR) in the forebrain is decreased in adult rats after adolescent intermittent ethanol (AIE) exposure. ChAT is the synthetic enzyme for acetylcholine and marks cholinergic neurons. Left, Quantification of ChAT+ IR cell density. Right, Representative photomicrograph of ChAT+ IR neurons in Ch1-Ch2 nuclei from adult control and AIE-exposed rats (Ch1-Ch2 from 0.70 to 0.20mm from bregma; Ch3-Ch4 from 0.7 to 0.30mm from bregma; Paxinos and Watson, 1998). Scale bar=100 μm. *P<.05 (Student’s t test). Control rats, n=9 to 10; AIE-exposed rats, n=12.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4368879&req=5

Figure 4: Choline acetyltransferase-positive immunoreactivity (ChAT+IR) in the forebrain is decreased in adult rats after adolescent intermittent ethanol (AIE) exposure. ChAT is the synthetic enzyme for acetylcholine and marks cholinergic neurons. Left, Quantification of ChAT+ IR cell density. Right, Representative photomicrograph of ChAT+ IR neurons in Ch1-Ch2 nuclei from adult control and AIE-exposed rats (Ch1-Ch2 from 0.70 to 0.20mm from bregma; Ch3-Ch4 from 0.7 to 0.30mm from bregma; Paxinos and Watson, 1998). Scale bar=100 μm. *P<.05 (Student’s t test). Control rats, n=9 to 10; AIE-exposed rats, n=12.

Mentions: AIE-exposed rats exhibited significantly lower levels of ChAT+ IR in cholinergic nuclei Ch1,2 (medial septal nucleus and nucleus of the vertical limb of the diagonal band, respectively; t20=2.39, P<.05) and Ch3,4 (lateral portion of the horizontal limb nucleus of the diagonal band and nucleus basalis and parts of diagonal band nuclei, respectively; t19=2.51, P<.05) of the basal forebrain (Figure 4). AIE-exposed rats also showed significantly lower levels of TH+ IR in the prelimbic cortex (t26=4.41, P<.05) but not in the infralimbic cortex (Figure 5). These findings demonstrate the long-term effects of AIE exposure on key neurotransmitters involved in risky choice.


Adolescent intermittent ethanol exposure is associated with increased risky choice and decreased dopaminergic and cholinergic neuron markers in adult rats.

Boutros N, Semenova S, Liu W, Crews FT, Markou A - Int. J. Neuropsychopharmacol. (2014)

Choline acetyltransferase-positive immunoreactivity (ChAT+IR) in the forebrain is decreased in adult rats after adolescent intermittent ethanol (AIE) exposure. ChAT is the synthetic enzyme for acetylcholine and marks cholinergic neurons. Left, Quantification of ChAT+ IR cell density. Right, Representative photomicrograph of ChAT+ IR neurons in Ch1-Ch2 nuclei from adult control and AIE-exposed rats (Ch1-Ch2 from 0.70 to 0.20mm from bregma; Ch3-Ch4 from 0.7 to 0.30mm from bregma; Paxinos and Watson, 1998). Scale bar=100 μm. *P<.05 (Student’s t test). Control rats, n=9 to 10; AIE-exposed rats, n=12.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4368879&req=5

Figure 4: Choline acetyltransferase-positive immunoreactivity (ChAT+IR) in the forebrain is decreased in adult rats after adolescent intermittent ethanol (AIE) exposure. ChAT is the synthetic enzyme for acetylcholine and marks cholinergic neurons. Left, Quantification of ChAT+ IR cell density. Right, Representative photomicrograph of ChAT+ IR neurons in Ch1-Ch2 nuclei from adult control and AIE-exposed rats (Ch1-Ch2 from 0.70 to 0.20mm from bregma; Ch3-Ch4 from 0.7 to 0.30mm from bregma; Paxinos and Watson, 1998). Scale bar=100 μm. *P<.05 (Student’s t test). Control rats, n=9 to 10; AIE-exposed rats, n=12.
Mentions: AIE-exposed rats exhibited significantly lower levels of ChAT+ IR in cholinergic nuclei Ch1,2 (medial septal nucleus and nucleus of the vertical limb of the diagonal band, respectively; t20=2.39, P<.05) and Ch3,4 (lateral portion of the horizontal limb nucleus of the diagonal band and nucleus basalis and parts of diagonal band nuclei, respectively; t19=2.51, P<.05) of the basal forebrain (Figure 4). AIE-exposed rats also showed significantly lower levels of TH+ IR in the prelimbic cortex (t26=4.41, P<.05) but not in the infralimbic cortex (Figure 5). These findings demonstrate the long-term effects of AIE exposure on key neurotransmitters involved in risky choice.

Bottom Line: Binge drinking is prevalent during adolescence and may have effects on the adult brain and behavior.Risky choice was negatively correlated with choline acetyltransferase, implicating decreased forebrain cholinergic activity in risky choice.The decreases in tyrosine hydroxylase and choline acetyltransferase immunoreactivity suggest that adolescent intermittent ethanol exposure has enduring neural effects that may lead to altered adult behaviors, such as increased risky decision making.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychiatry, University of California San Diego, La Jolla, California (Drs Boutros, Semenova, Markou); Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina (Drs Liu, Crews).

Show MeSH
Related in: MedlinePlus