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Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.

Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A - In Vitro Cell. Dev. Biol. Anim. (2014)

Bottom Line: In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated.Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied.Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

View Article: PubMed Central - PubMed

Affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences Wroclaw, Kozuchowska 5b, 51-631, Wroclaw, Poland, monika.maredziak@gmail.com.

ABSTRACT
The aim of this work study was to evaluate the cytophysiological activity of equine adipose-derived stem cells (ASCs) cultured under conditions of static magnetic field. Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated. Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied. Resazurin-based assay indicated that the cells cultured in the magnetic field reached the population doubling time earlier and colony-forming potential of equine ASCs was higher when cells were cultured under magnetic field conditions. Morphological and ultrastructural examination of equine ASCs showed that the exposure to magnetic field did not cause any significant changes in cell morphology whereas the polarity of the cells was observed under the magnetic field conditions in ultrastructural examinations. Exposition to MF resulted in a considerable increase in the number of secreted MVs-we have clearly observed the differences between the numbers of MVs shed from the cells cultured under MF in comparison to the control culture and were rich in growth factors. Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

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Related in: MedlinePlus

SEM images of equine ASC cultures exposed to magnetic field (A) and the control culture (B); MVs secreted from the cells cultured under magnetic field (C) and control conditions (D). MVs diameters and appropriate scale bars are indicated.
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Fig3: SEM images of equine ASC cultures exposed to magnetic field (A) and the control culture (B); MVs secreted from the cells cultured under magnetic field (C) and control conditions (D). MVs diameters and appropriate scale bars are indicated.

Mentions: Exposition to MF resulted in a considerable increase in the number of secreted MVs (size of 100–1,000 nm) in equine ASCs. These observations were confirmed by electron microscopy as well as by computer-assisted method using a Java-based image processing software ImageJ v1.48. Although in the majority of electronographs microvesicles were located around the nuclei (Figs. 3A, B and 4A–D), the wide spectrum of MVs was also observed at the border area of cells (Fig. 4C, D). Measurements of MVs diameters demonstrated the presence of particles of size greater than 100 nm, which were predominant in the samples analyzed (Fig. 3C, D). MVs greater than 100 nm were significantly more common in the cells exposed to MF in comparison to control (Figs. 4A–D and 5G–I). Since significant differences in the number of microvesicles have been attributed to major changes in physiological activity, we assessed the number of MVs per cell as the active cell area (Fig. 4). The percentage of MV area was determined using ImageJ software, and it amounted to about 20% in the samples exposed to MF. In the control samples, the percentage of the active area reached 11% (Fig. 4G, H).Figure 3.


Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.

Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A - In Vitro Cell. Dev. Biol. Anim. (2014)

SEM images of equine ASC cultures exposed to magnetic field (A) and the control culture (B); MVs secreted from the cells cultured under magnetic field (C) and control conditions (D). MVs diameters and appropriate scale bars are indicated.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4368852&req=5

Fig3: SEM images of equine ASC cultures exposed to magnetic field (A) and the control culture (B); MVs secreted from the cells cultured under magnetic field (C) and control conditions (D). MVs diameters and appropriate scale bars are indicated.
Mentions: Exposition to MF resulted in a considerable increase in the number of secreted MVs (size of 100–1,000 nm) in equine ASCs. These observations were confirmed by electron microscopy as well as by computer-assisted method using a Java-based image processing software ImageJ v1.48. Although in the majority of electronographs microvesicles were located around the nuclei (Figs. 3A, B and 4A–D), the wide spectrum of MVs was also observed at the border area of cells (Fig. 4C, D). Measurements of MVs diameters demonstrated the presence of particles of size greater than 100 nm, which were predominant in the samples analyzed (Fig. 3C, D). MVs greater than 100 nm were significantly more common in the cells exposed to MF in comparison to control (Figs. 4A–D and 5G–I). Since significant differences in the number of microvesicles have been attributed to major changes in physiological activity, we assessed the number of MVs per cell as the active cell area (Fig. 4). The percentage of MV area was determined using ImageJ software, and it amounted to about 20% in the samples exposed to MF. In the control samples, the percentage of the active area reached 11% (Fig. 4G, H).Figure 3.

Bottom Line: In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated.Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied.Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

View Article: PubMed Central - PubMed

Affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences Wroclaw, Kozuchowska 5b, 51-631, Wroclaw, Poland, monika.maredziak@gmail.com.

ABSTRACT
The aim of this work study was to evaluate the cytophysiological activity of equine adipose-derived stem cells (ASCs) cultured under conditions of static magnetic field. Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated. Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied. Resazurin-based assay indicated that the cells cultured in the magnetic field reached the population doubling time earlier and colony-forming potential of equine ASCs was higher when cells were cultured under magnetic field conditions. Morphological and ultrastructural examination of equine ASCs showed that the exposure to magnetic field did not cause any significant changes in cell morphology whereas the polarity of the cells was observed under the magnetic field conditions in ultrastructural examinations. Exposition to MF resulted in a considerable increase in the number of secreted MVs-we have clearly observed the differences between the numbers of MVs shed from the cells cultured under MF in comparison to the control culture and were rich in growth factors. Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

Show MeSH
Related in: MedlinePlus