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Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.

Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A - In Vitro Cell. Dev. Biol. Anim. (2014)

Bottom Line: In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated.Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied.Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

View Article: PubMed Central - PubMed

Affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences Wroclaw, Kozuchowska 5b, 51-631, Wroclaw, Poland, monika.maredziak@gmail.com.

ABSTRACT
The aim of this work study was to evaluate the cytophysiological activity of equine adipose-derived stem cells (ASCs) cultured under conditions of static magnetic field. Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated. Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied. Resazurin-based assay indicated that the cells cultured in the magnetic field reached the population doubling time earlier and colony-forming potential of equine ASCs was higher when cells were cultured under magnetic field conditions. Morphological and ultrastructural examination of equine ASCs showed that the exposure to magnetic field did not cause any significant changes in cell morphology whereas the polarity of the cells was observed under the magnetic field conditions in ultrastructural examinations. Exposition to MF resulted in a considerable increase in the number of secreted MVs-we have clearly observed the differences between the numbers of MVs shed from the cells cultured under MF in comparison to the control culture and were rich in growth factors. Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

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Comparison of equine ASC morphology nuclei distribution (A, B) and ultrastructure (C, D) after exposure to a magnetic field (A, C) and under control conditions (B, D). Red asterisks indicate displacement of the nuclei to the peripheral part of the cells. Appropriate scale bars are indicated in each panel.
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Fig2: Comparison of equine ASC morphology nuclei distribution (A, B) and ultrastructure (C, D) after exposure to a magnetic field (A, C) and under control conditions (B, D). Red asterisks indicate displacement of the nuclei to the peripheral part of the cells. Appropriate scale bars are indicated in each panel.

Mentions: Morphological examination of equine ASCs revealed that the exposure to magnetic field did not cause any significant changes in cell morphology. The cells exposed to magnetic field exhibited morphological features similar to those of the control cells, maintaining typical fibroblast-like morphology. The cells formed a homogenous monolayer of the bipolar spindle-like cells with a regular pattern (Fig. 2). DAPI staining of the nuclei indicated that the cells cultured in the presence of a magnetic field had asymmetrically located nuclei, translocated to one of the cell poles (Fig. 2A), whereas in the cells cultured in the control conditions, the nuclei were positioned centrally (Fig. 2B). Cells cultured in the presence of MF adhered evenly to the surface of the well and did not cluster. Cells that were not exposed to MF densely covered the central part of the well and closely adhered to each other.Figure 2.


Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.

Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A - In Vitro Cell. Dev. Biol. Anim. (2014)

Comparison of equine ASC morphology nuclei distribution (A, B) and ultrastructure (C, D) after exposure to a magnetic field (A, C) and under control conditions (B, D). Red asterisks indicate displacement of the nuclei to the peripheral part of the cells. Appropriate scale bars are indicated in each panel.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4368852&req=5

Fig2: Comparison of equine ASC morphology nuclei distribution (A, B) and ultrastructure (C, D) after exposure to a magnetic field (A, C) and under control conditions (B, D). Red asterisks indicate displacement of the nuclei to the peripheral part of the cells. Appropriate scale bars are indicated in each panel.
Mentions: Morphological examination of equine ASCs revealed that the exposure to magnetic field did not cause any significant changes in cell morphology. The cells exposed to magnetic field exhibited morphological features similar to those of the control cells, maintaining typical fibroblast-like morphology. The cells formed a homogenous monolayer of the bipolar spindle-like cells with a regular pattern (Fig. 2). DAPI staining of the nuclei indicated that the cells cultured in the presence of a magnetic field had asymmetrically located nuclei, translocated to one of the cell poles (Fig. 2A), whereas in the cells cultured in the control conditions, the nuclei were positioned centrally (Fig. 2B). Cells cultured in the presence of MF adhered evenly to the surface of the well and did not cluster. Cells that were not exposed to MF densely covered the central part of the well and closely adhered to each other.Figure 2.

Bottom Line: In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated.Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied.Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

View Article: PubMed Central - PubMed

Affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences Wroclaw, Kozuchowska 5b, 51-631, Wroclaw, Poland, monika.maredziak@gmail.com.

ABSTRACT
The aim of this work study was to evaluate the cytophysiological activity of equine adipose-derived stem cells (ASCs) cultured under conditions of static magnetic field. Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated. Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied. Resazurin-based assay indicated that the cells cultured in the magnetic field reached the population doubling time earlier and colony-forming potential of equine ASCs was higher when cells were cultured under magnetic field conditions. Morphological and ultrastructural examination of equine ASCs showed that the exposure to magnetic field did not cause any significant changes in cell morphology whereas the polarity of the cells was observed under the magnetic field conditions in ultrastructural examinations. Exposition to MF resulted in a considerable increase in the number of secreted MVs-we have clearly observed the differences between the numbers of MVs shed from the cells cultured under MF in comparison to the control culture and were rich in growth factors. Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

Show MeSH
Related in: MedlinePlus