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Enhancement of protective efficacy through adenoviral vectored vaccine priming and protein boosting strategy encoding triosephosphate isomerase (SjTPI) against Schistosoma japonicum in mice.

Dai Y, Wang X, Tang J, Zhao S, Xing Y, Dai J, Jin X, Zhu Y - PLoS ONE (2015)

Bottom Line: Furthermore, the longevity of protective efficacy was also determined.Results showed that the rAdV-SjTPI.opt priming-rSjTPI boosting strategy elicited higher levels of specific IgG responses and broad-spectrum specific cellular immune responses.The protective efficacy could reach up to nearly 70% and 50% of protection could be observed at 10 weeks after the last immunization in mice.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.

ABSTRACT

Background: Schistosomiasis japonica is a zoonotic parasitic disease; developing transmission blocking veterinary vaccines are urgently needed for the prevention and control of schistosomiasis in China. Heterologous prime-boost strategy, a novel vaccination approach, is more effective in enhancing vaccine efficacy against multiple pathogens. In the present study, we established a novel heterologous prime-boost vaccination strategy, the rAdV-SjTPI.opt intramuscular priming and rSjTPI subcutaneous boosting strategy, and evaluated its protective efficacy against Schistosoma japonicum in mice.

Methodology/principal findings: Adenoviral vectored vaccine (rAdV-SjTPI.opt) and recombinant protein vaccine (rSjTPI) were prepared and used in different combinations as vaccines in a mouse model. The specific immune responses and protective efficacies were evaluated. Furthermore, the longevity of protective efficacy was also determined. Results showed that the rAdV-SjTPI.opt priming-rSjTPI boosting strategy elicited higher levels of specific IgG responses and broad-spectrum specific cellular immune responses. The protective efficacy could reach up to nearly 70% and 50% of protection could be observed at 10 weeks after the last immunization in mice.

Conclusions/significance: The rAdV-SjTPI.opt intramuscular priming-rSjTPI subcutaneous boosting vaccination strategy is a novel, highly efficient, and stable approach to developing vaccines against Schistosoma japonicum infections in China.

No MeSH data available.


Related in: MedlinePlus

The single egg granuloma responses in liver induced by Ad vector, rSjTPI, rAdV-SjTPI.opt, and rAdV-SjTPI.opt priming-rSjTPI boosting immunization.(A) Representative granuloma of each group, induced by a single egg in liver (magnification factor 10×10); (B) Areas of the single egg granuloma in liver. Data is presented as the mean ± standard deviation (SD). *P<0.05; **P<0.01.
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pone.0120792.g003: The single egg granuloma responses in liver induced by Ad vector, rSjTPI, rAdV-SjTPI.opt, and rAdV-SjTPI.opt priming-rSjTPI boosting immunization.(A) Representative granuloma of each group, induced by a single egg in liver (magnification factor 10×10); (B) Areas of the single egg granuloma in liver. Data is presented as the mean ± standard deviation (SD). *P<0.05; **P<0.01.

Mentions: Results of protective efficacy in each group were shown in Fig. 3 and Table 1. Compared with the control group and the Ad vector immunization, the rAdV-SjTPI.opt, rSjTPI, and rAdV-SjTPI.opt priming-rSjTPI boosting groups resulted in lower numbers of adult and female worms and eggs in the liver (see Table 1). Worm and egg burdens in the liver were significantly lower after immunization with rAdV-SjTPI.opt than after immunization with rSjTPI (see Table 1). The number of worms and eggs in the liver in the rAdV-SjTPI.opt priming-rSjTPI boosting group were significantly lower than that in the rSjTPI and rAdV-SjTPI.opt groups. The area of single egg granuloma in the rAdV-SjTPI.opt, rSjTPI and rAdV-SjTPI.opt priming-rSjTPI boosting groups was smaller than that in the Ad vector or control group. The area of single egg granuloma was smaller in the rAdV-SjTPI.opt group than in the rSjTPI group. However, the smallest area of single egg granuloma was observed in the rAdV-SjTPI.opt priming-rSjTPI boosting immunization group (see Fig. 3A and B). These findings suggest that the rAdV-SjTPI.opt priming-rSjTPI boosting vaccination strategy could synergistically enhance the protective efficacy against Schistosoma japonicum in a challenge infection of mice.


Enhancement of protective efficacy through adenoviral vectored vaccine priming and protein boosting strategy encoding triosephosphate isomerase (SjTPI) against Schistosoma japonicum in mice.

Dai Y, Wang X, Tang J, Zhao S, Xing Y, Dai J, Jin X, Zhu Y - PLoS ONE (2015)

The single egg granuloma responses in liver induced by Ad vector, rSjTPI, rAdV-SjTPI.opt, and rAdV-SjTPI.opt priming-rSjTPI boosting immunization.(A) Representative granuloma of each group, induced by a single egg in liver (magnification factor 10×10); (B) Areas of the single egg granuloma in liver. Data is presented as the mean ± standard deviation (SD). *P<0.05; **P<0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4368607&req=5

pone.0120792.g003: The single egg granuloma responses in liver induced by Ad vector, rSjTPI, rAdV-SjTPI.opt, and rAdV-SjTPI.opt priming-rSjTPI boosting immunization.(A) Representative granuloma of each group, induced by a single egg in liver (magnification factor 10×10); (B) Areas of the single egg granuloma in liver. Data is presented as the mean ± standard deviation (SD). *P<0.05; **P<0.01.
Mentions: Results of protective efficacy in each group were shown in Fig. 3 and Table 1. Compared with the control group and the Ad vector immunization, the rAdV-SjTPI.opt, rSjTPI, and rAdV-SjTPI.opt priming-rSjTPI boosting groups resulted in lower numbers of adult and female worms and eggs in the liver (see Table 1). Worm and egg burdens in the liver were significantly lower after immunization with rAdV-SjTPI.opt than after immunization with rSjTPI (see Table 1). The number of worms and eggs in the liver in the rAdV-SjTPI.opt priming-rSjTPI boosting group were significantly lower than that in the rSjTPI and rAdV-SjTPI.opt groups. The area of single egg granuloma in the rAdV-SjTPI.opt, rSjTPI and rAdV-SjTPI.opt priming-rSjTPI boosting groups was smaller than that in the Ad vector or control group. The area of single egg granuloma was smaller in the rAdV-SjTPI.opt group than in the rSjTPI group. However, the smallest area of single egg granuloma was observed in the rAdV-SjTPI.opt priming-rSjTPI boosting immunization group (see Fig. 3A and B). These findings suggest that the rAdV-SjTPI.opt priming-rSjTPI boosting vaccination strategy could synergistically enhance the protective efficacy against Schistosoma japonicum in a challenge infection of mice.

Bottom Line: Furthermore, the longevity of protective efficacy was also determined.Results showed that the rAdV-SjTPI.opt priming-rSjTPI boosting strategy elicited higher levels of specific IgG responses and broad-spectrum specific cellular immune responses.The protective efficacy could reach up to nearly 70% and 50% of protection could be observed at 10 weeks after the last immunization in mice.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.

ABSTRACT

Background: Schistosomiasis japonica is a zoonotic parasitic disease; developing transmission blocking veterinary vaccines are urgently needed for the prevention and control of schistosomiasis in China. Heterologous prime-boost strategy, a novel vaccination approach, is more effective in enhancing vaccine efficacy against multiple pathogens. In the present study, we established a novel heterologous prime-boost vaccination strategy, the rAdV-SjTPI.opt intramuscular priming and rSjTPI subcutaneous boosting strategy, and evaluated its protective efficacy against Schistosoma japonicum in mice.

Methodology/principal findings: Adenoviral vectored vaccine (rAdV-SjTPI.opt) and recombinant protein vaccine (rSjTPI) were prepared and used in different combinations as vaccines in a mouse model. The specific immune responses and protective efficacies were evaluated. Furthermore, the longevity of protective efficacy was also determined. Results showed that the rAdV-SjTPI.opt priming-rSjTPI boosting strategy elicited higher levels of specific IgG responses and broad-spectrum specific cellular immune responses. The protective efficacy could reach up to nearly 70% and 50% of protection could be observed at 10 weeks after the last immunization in mice.

Conclusions/significance: The rAdV-SjTPI.opt intramuscular priming-rSjTPI subcutaneous boosting vaccination strategy is a novel, highly efficient, and stable approach to developing vaccines against Schistosoma japonicum infections in China.

No MeSH data available.


Related in: MedlinePlus