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Characterization of Tunga penetrans antigens in selected epidemic areas in Murang'a county in Kenya.

Mwangi JN, Ozwara HS, Motiso JM, Gicheru MM - PLoS Negl Trop Dis (2015)

Bottom Line: These results were comparable to results of immunoelectrphoresis.These results are important since they would help understand immunological behavior of the parasites.This would help to create basis for designing and improving approaches against jiggers such as development of immune prophylaxis to complement social science approaches that is mainly concerned with maintenance of high standards of hygiene.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoological sciences, Kenyatta University, Nairobi, Kenya.

ABSTRACT
Tunga penetrans are fleas that cause tungiasis, a condition characterized by high transmission rate due to poor housing conditions, social neglect and inadequate health care in economically disadvantaged communities in developing countries. This study therefore aimed at characterizing jiggers antigens to identify immunodominant ones to help understand immunological behavior of the parasite that would otherwise be important in future control of the parasite. Samples were gravid fleas and blood samples from infested individuals in Kahuro and Murang'a East district in Murang'a County. Freeze and thaw was used to extract soluble proteins from the fleas. Ouchterlony Double immunodiffusion was used to assess antigen-antibody reactions between extracted soluble protein and the serum from immunized rats, Rattus norvegicus prior to analysis of human sera. These results were comparable to results of immunoelectrphoresis. Jigger protein isolates were analyzed in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis technique (SDS-PAGE), against Pharmacia standard protein markers. Further analysis of jigger antigens against pooled human sera from infested victims in Western blot revealed three immunodominant antigens. Using simple regression analysis molecular weights of the three immunodominant antigens were estimated as 51.795, 23.395 and 15.38 kDa respectively. These results are important since they would help understand immunological behavior of the parasites. This would help to create basis for designing and improving approaches against jiggers such as development of immune prophylaxis to complement social science approaches that is mainly concerned with maintenance of high standards of hygiene.

No MeSH data available.


Related in: MedlinePlus

Analysis of T. penetrans antigens in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE).The positive control was a low molecular weight marker from Pharmacia Ltd. Negative control was Phosphate Buffered Saline (PBS). Sample proteins were jigger extracts prepared in dilutions of 1:2, 1:4, 1:5, 1:6 and 1:12 respectively.
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pntd.0003517.g003: Analysis of T. penetrans antigens in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE).The positive control was a low molecular weight marker from Pharmacia Ltd. Negative control was Phosphate Buffered Saline (PBS). Sample proteins were jigger extracts prepared in dilutions of 1:2, 1:4, 1:5, 1:6 and 1:12 respectively.

Mentions: Tunga penetrans antigens were further analyzed in SDS—PAGE (Fig. 3). Result shows that separated jigger antigenic protein are all of medium to low molecular weights. Separated T. penetrans antigens were further characterized in Western blot (Fig. 4). Strips numbered 1–5 in I and strips numbered 7–11 in II are replicas comparing results of Western blot whereby T. penetrans antigens reacted with pooled human sera from infested victims. Result shows the most immunodominant antigens labeled A, B, and C in strip 5 (Fig. 4, I) as compared to strip 11 (Fig. 4, II).


Characterization of Tunga penetrans antigens in selected epidemic areas in Murang'a county in Kenya.

Mwangi JN, Ozwara HS, Motiso JM, Gicheru MM - PLoS Negl Trop Dis (2015)

Analysis of T. penetrans antigens in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE).The positive control was a low molecular weight marker from Pharmacia Ltd. Negative control was Phosphate Buffered Saline (PBS). Sample proteins were jigger extracts prepared in dilutions of 1:2, 1:4, 1:5, 1:6 and 1:12 respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4368547&req=5

pntd.0003517.g003: Analysis of T. penetrans antigens in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE).The positive control was a low molecular weight marker from Pharmacia Ltd. Negative control was Phosphate Buffered Saline (PBS). Sample proteins were jigger extracts prepared in dilutions of 1:2, 1:4, 1:5, 1:6 and 1:12 respectively.
Mentions: Tunga penetrans antigens were further analyzed in SDS—PAGE (Fig. 3). Result shows that separated jigger antigenic protein are all of medium to low molecular weights. Separated T. penetrans antigens were further characterized in Western blot (Fig. 4). Strips numbered 1–5 in I and strips numbered 7–11 in II are replicas comparing results of Western blot whereby T. penetrans antigens reacted with pooled human sera from infested victims. Result shows the most immunodominant antigens labeled A, B, and C in strip 5 (Fig. 4, I) as compared to strip 11 (Fig. 4, II).

Bottom Line: These results were comparable to results of immunoelectrphoresis.These results are important since they would help understand immunological behavior of the parasites.This would help to create basis for designing and improving approaches against jiggers such as development of immune prophylaxis to complement social science approaches that is mainly concerned with maintenance of high standards of hygiene.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoological sciences, Kenyatta University, Nairobi, Kenya.

ABSTRACT
Tunga penetrans are fleas that cause tungiasis, a condition characterized by high transmission rate due to poor housing conditions, social neglect and inadequate health care in economically disadvantaged communities in developing countries. This study therefore aimed at characterizing jiggers antigens to identify immunodominant ones to help understand immunological behavior of the parasite that would otherwise be important in future control of the parasite. Samples were gravid fleas and blood samples from infested individuals in Kahuro and Murang'a East district in Murang'a County. Freeze and thaw was used to extract soluble proteins from the fleas. Ouchterlony Double immunodiffusion was used to assess antigen-antibody reactions between extracted soluble protein and the serum from immunized rats, Rattus norvegicus prior to analysis of human sera. These results were comparable to results of immunoelectrphoresis. Jigger protein isolates were analyzed in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis technique (SDS-PAGE), against Pharmacia standard protein markers. Further analysis of jigger antigens against pooled human sera from infested victims in Western blot revealed three immunodominant antigens. Using simple regression analysis molecular weights of the three immunodominant antigens were estimated as 51.795, 23.395 and 15.38 kDa respectively. These results are important since they would help understand immunological behavior of the parasites. This would help to create basis for designing and improving approaches against jiggers such as development of immune prophylaxis to complement social science approaches that is mainly concerned with maintenance of high standards of hygiene.

No MeSH data available.


Related in: MedlinePlus