Prenatal inhibition of the kynurenine pathway leads to structural changes in the hippocampus of adult rat offspring.
Bottom Line: Golgi-Cox silver staining revealed decreased overall numbers and lengths of CA1 basal dendrites and secondary basal dendrites, together with fewer basal dendritic spines and less overall dendritic complexity in the basal arbour.The number of neuron terminals staining for vesicular glutamate transporter (VGLUT)-1 and VGLUT-2 was increased by Ro61-8048, with no change in expression of vesicular GABA transporter or its co-localisation with vesicle-associated membrane protein-1.These data support the view that constitutive kynurenine metabolism normally plays a role in early embryonic brain development, and that interfering with it has profound consequences for neuronal structure and morphology, lasting into adulthood.
Affiliation: Institute of Neuroscience and Psychology, West Medical Building, University of Glasgow, Glasgow, G12 8QQ, UK.Show MeSH
Mentions: In the apical dendritic tree, the total spine density was no different in the Ro61-8048-exposed tissue (Fig.2A), although separate analysis of the thin and thick (mushroom-shaped) spines indicated a significant reduction in the number of the latter (two-tailed t-test, P = 0.007) (Fig.2B) with no significant difference in the population of thin spines (Fig.2C; two-tailed t-test, P = 0.54). The density of spines on the basal dendrites of CA1 neurons was significantly lower in tissue from rats exposed to Ro61-8048 in utero than in control tissue (two-tailed t-test, P = 0.036) (Fig.2D), but with no differential loss of thin spines (two-tailed t-test, P = 0.07) (Fig.2E) or thick mushroom spines (P = 0.75; Fig.2F).
Affiliation: Institute of Neuroscience and Psychology, West Medical Building, University of Glasgow, Glasgow, G12 8QQ, UK.