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Prenatal inhibition of the kynurenine pathway leads to structural changes in the hippocampus of adult rat offspring.

Khalil OS, Pisar M, Forrest CM, Vincenten MC, Darlington LG, Stone TW - Eur. J. Neurosci. (2014)

Bottom Line: Golgi-Cox silver staining revealed decreased overall numbers and lengths of CA1 basal dendrites and secondary basal dendrites, together with fewer basal dendritic spines and less overall dendritic complexity in the basal arbour.The number of neuron terminals staining for vesicular glutamate transporter (VGLUT)-1 and VGLUT-2 was increased by Ro61-8048, with no change in expression of vesicular GABA transporter or its co-localisation with vesicle-associated membrane protein-1.These data support the view that constitutive kynurenine metabolism normally plays a role in early embryonic brain development, and that interfering with it has profound consequences for neuronal structure and morphology, lasting into adulthood.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience and Psychology, West Medical Building, University of Glasgow, Glasgow, G12 8QQ, UK.

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Morphological analysis of silver-stained neurons in the hippocampal CA1 region. Tracings are shown of typical pyramidal neurons from a rat treated with vehicle (1) and a rat treated with Ro61-8048 (2). Scale bar: 100 μm. (A) The total number of dendrites in the apical and basal dendritic trees. (B) The total length of the dendrites in the apical and basal dendritic trees. (C) The number of primary and secondary dendrites in the basal tree, with significant differences between control and vehicle-treated tissue (D) This indicates the absence of any difference in the apical dendrites. (E and F) A similar analysis of dendritic length. Data from control, vehicle-treated rats are shown as open bars, and data from Ro61-8048-treated rats are shown as shaded bars. (G and H) Analysis of dendritic complexity is illustrated for the apical (G) and basal (H) dendrites. *P < 0.05; **P < 0.01; ***P < 0.001.
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fig01: Morphological analysis of silver-stained neurons in the hippocampal CA1 region. Tracings are shown of typical pyramidal neurons from a rat treated with vehicle (1) and a rat treated with Ro61-8048 (2). Scale bar: 100 μm. (A) The total number of dendrites in the apical and basal dendritic trees. (B) The total length of the dendrites in the apical and basal dendritic trees. (C) The number of primary and secondary dendrites in the basal tree, with significant differences between control and vehicle-treated tissue (D) This indicates the absence of any difference in the apical dendrites. (E and F) A similar analysis of dendritic length. Data from control, vehicle-treated rats are shown as open bars, and data from Ro61-8048-treated rats are shown as shaded bars. (G and H) Analysis of dendritic complexity is illustrated for the apical (G) and basal (H) dendrites. *P < 0.05; **P < 0.01; ***P < 0.001.

Mentions: Several aspects of the dendritic tree were analysed. Counting each branch point generated a value for the number of dendritic branches in the tree. This measurement was made separately for dendrites in the apical and basal regions of CA1 pyramidal cells, with a significant loss being observed in the number of basal but not of apical dendrites (Fig.1A). A small but highly significant decrease was also noted in the length of the basal dendritic tree (Fig.1B).


Prenatal inhibition of the kynurenine pathway leads to structural changes in the hippocampus of adult rat offspring.

Khalil OS, Pisar M, Forrest CM, Vincenten MC, Darlington LG, Stone TW - Eur. J. Neurosci. (2014)

Morphological analysis of silver-stained neurons in the hippocampal CA1 region. Tracings are shown of typical pyramidal neurons from a rat treated with vehicle (1) and a rat treated with Ro61-8048 (2). Scale bar: 100 μm. (A) The total number of dendrites in the apical and basal dendritic trees. (B) The total length of the dendrites in the apical and basal dendritic trees. (C) The number of primary and secondary dendrites in the basal tree, with significant differences between control and vehicle-treated tissue (D) This indicates the absence of any difference in the apical dendrites. (E and F) A similar analysis of dendritic length. Data from control, vehicle-treated rats are shown as open bars, and data from Ro61-8048-treated rats are shown as shaded bars. (G and H) Analysis of dendritic complexity is illustrated for the apical (G) and basal (H) dendrites. *P < 0.05; **P < 0.01; ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4368408&req=5

fig01: Morphological analysis of silver-stained neurons in the hippocampal CA1 region. Tracings are shown of typical pyramidal neurons from a rat treated with vehicle (1) and a rat treated with Ro61-8048 (2). Scale bar: 100 μm. (A) The total number of dendrites in the apical and basal dendritic trees. (B) The total length of the dendrites in the apical and basal dendritic trees. (C) The number of primary and secondary dendrites in the basal tree, with significant differences between control and vehicle-treated tissue (D) This indicates the absence of any difference in the apical dendrites. (E and F) A similar analysis of dendritic length. Data from control, vehicle-treated rats are shown as open bars, and data from Ro61-8048-treated rats are shown as shaded bars. (G and H) Analysis of dendritic complexity is illustrated for the apical (G) and basal (H) dendrites. *P < 0.05; **P < 0.01; ***P < 0.001.
Mentions: Several aspects of the dendritic tree were analysed. Counting each branch point generated a value for the number of dendritic branches in the tree. This measurement was made separately for dendrites in the apical and basal regions of CA1 pyramidal cells, with a significant loss being observed in the number of basal but not of apical dendrites (Fig.1A). A small but highly significant decrease was also noted in the length of the basal dendritic tree (Fig.1B).

Bottom Line: Golgi-Cox silver staining revealed decreased overall numbers and lengths of CA1 basal dendrites and secondary basal dendrites, together with fewer basal dendritic spines and less overall dendritic complexity in the basal arbour.The number of neuron terminals staining for vesicular glutamate transporter (VGLUT)-1 and VGLUT-2 was increased by Ro61-8048, with no change in expression of vesicular GABA transporter or its co-localisation with vesicle-associated membrane protein-1.These data support the view that constitutive kynurenine metabolism normally plays a role in early embryonic brain development, and that interfering with it has profound consequences for neuronal structure and morphology, lasting into adulthood.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience and Psychology, West Medical Building, University of Glasgow, Glasgow, G12 8QQ, UK.

Show MeSH
Related in: MedlinePlus