Prenatal inhibition of the kynurenine pathway leads to structural changes in the hippocampus of adult rat offspring.
Bottom Line: Golgi-Cox silver staining revealed decreased overall numbers and lengths of CA1 basal dendrites and secondary basal dendrites, together with fewer basal dendritic spines and less overall dendritic complexity in the basal arbour.The number of neuron terminals staining for vesicular glutamate transporter (VGLUT)-1 and VGLUT-2 was increased by Ro61-8048, with no change in expression of vesicular GABA transporter or its co-localisation with vesicle-associated membrane protein-1.These data support the view that constitutive kynurenine metabolism normally plays a role in early embryonic brain development, and that interfering with it has profound consequences for neuronal structure and morphology, lasting into adulthood.
Affiliation: Institute of Neuroscience and Psychology, West Medical Building, University of Glasgow, Glasgow, G12 8QQ, UK.Show MeSH
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Mentions: Several aspects of the dendritic tree were analysed. Counting each branch point generated a value for the number of dendritic branches in the tree. This measurement was made separately for dendrites in the apical and basal regions of CA1 pyramidal cells, with a significant loss being observed in the number of basal but not of apical dendrites (Fig.1A). A small but highly significant decrease was also noted in the length of the basal dendritic tree (Fig.1B).
Affiliation: Institute of Neuroscience and Psychology, West Medical Building, University of Glasgow, Glasgow, G12 8QQ, UK.