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Role of 5-HT3 receptor on food intake in fed and fasted mice.

Li B, Shao D, Luo Y, Wang P, Liu C, Zhang X, Cui R - PLoS ONE (2015)

Bottom Line: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior.However, the relative contribution of 5-HT3 receptor remains unclear.Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

View Article: PubMed Central - PubMed

Affiliation: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.

ABSTRACT

Background: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior. However, the relative contribution of 5-HT3 receptor remains unclear. The present study was aimed to investigate the role of 5-HT3 receptor in control of feeding behavior in fed and fasted mice.

Methodology/principal findings: Food intake and expression of c-Fos, tyrosine hydroxylase (TH), proopiomelanocortin (POMC) and 5-HT in the brain were examined after acute treatment with 5-HT3 receptor agonist SR-57227 alone or in combination with 5-HT3 receptor antagonist ondansetron. Food intake was significantly inhibited within 3 h after acute treatment with SR 57227 in fasted mice but not fed mice, and this inhibition was blocked by ondansetron. Immunohistochemical study revealed that fasting-induced c-Fos expression was further enhanced by SR 57227 in the brainstem and the hypothalamus, and this enhancement was also blocked by ondansetron. Furthermore, the fasting-induced downregulation of POMC expression in the hypothalamus and the TH expression in the brain stem was blocked by SR 57227 in the fasted mice, and this effect of SR 57227 was also antagonized by ondansetron.

Conclusion/significance: Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

No MeSH data available.


Related in: MedlinePlus

c-Fos expression in hypothalamus of fed and fasted mice.Typical photomicrographs of c-Fos expression in hypothalamus of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in hypothalamus of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). PVN: paraventricular nucleus of hypothalamus; DMH: dorsomedial hypothalamus; VMH: ventromedial hypothalamus; LHA: lateral hypothalamic area; PALM: paraventricular hypothalamic nucleus, lateral magnocellular part; PAMM: Paraventricular hypothalamic nucleus, medial magnocellular part; ARC: arcuate nuclei of the hypothalamus; 3V: third ventricle.Values are means ± SEM (n = 7–8). #, P < 0.05; ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; ** P < 0.01; ***, P < 0.001 vs. fasting control group.
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pone.0121473.g003: c-Fos expression in hypothalamus of fed and fasted mice.Typical photomicrographs of c-Fos expression in hypothalamus of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in hypothalamus of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). PVN: paraventricular nucleus of hypothalamus; DMH: dorsomedial hypothalamus; VMH: ventromedial hypothalamus; LHA: lateral hypothalamic area; PALM: paraventricular hypothalamic nucleus, lateral magnocellular part; PAMM: Paraventricular hypothalamic nucleus, medial magnocellular part; ARC: arcuate nuclei of the hypothalamus; 3V: third ventricle.Values are means ± SEM (n = 7–8). #, P < 0.05; ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; ** P < 0.01; ***, P < 0.001 vs. fasting control group.

Mentions: As shown by one-way ANOVA, in fed mice SR-57227 significantly increased c-Fos expression in most areas of hypothalamus, including PVN, DMH, VMH, LHA and ARC (F(3, 28) = 67.12, P<0.01) (Fig. 3A and 3B). Fasting increased c-Fos expression in these brain areas except LHA, and the c-Fos expressions were enhanced by SR-57227 (Fig. 3C and D). These enhancements of c-Fos expression induced by SR-57227 were attenuated by ondansetron in both fed and fasting mice (Fig. 3A–3D).


Role of 5-HT3 receptor on food intake in fed and fasted mice.

Li B, Shao D, Luo Y, Wang P, Liu C, Zhang X, Cui R - PLoS ONE (2015)

c-Fos expression in hypothalamus of fed and fasted mice.Typical photomicrographs of c-Fos expression in hypothalamus of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in hypothalamus of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). PVN: paraventricular nucleus of hypothalamus; DMH: dorsomedial hypothalamus; VMH: ventromedial hypothalamus; LHA: lateral hypothalamic area; PALM: paraventricular hypothalamic nucleus, lateral magnocellular part; PAMM: Paraventricular hypothalamic nucleus, medial magnocellular part; ARC: arcuate nuclei of the hypothalamus; 3V: third ventricle.Values are means ± SEM (n = 7–8). #, P < 0.05; ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; ** P < 0.01; ***, P < 0.001 vs. fasting control group.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4366218&req=5

pone.0121473.g003: c-Fos expression in hypothalamus of fed and fasted mice.Typical photomicrographs of c-Fos expression in hypothalamus of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in hypothalamus of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). PVN: paraventricular nucleus of hypothalamus; DMH: dorsomedial hypothalamus; VMH: ventromedial hypothalamus; LHA: lateral hypothalamic area; PALM: paraventricular hypothalamic nucleus, lateral magnocellular part; PAMM: Paraventricular hypothalamic nucleus, medial magnocellular part; ARC: arcuate nuclei of the hypothalamus; 3V: third ventricle.Values are means ± SEM (n = 7–8). #, P < 0.05; ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; ** P < 0.01; ***, P < 0.001 vs. fasting control group.
Mentions: As shown by one-way ANOVA, in fed mice SR-57227 significantly increased c-Fos expression in most areas of hypothalamus, including PVN, DMH, VMH, LHA and ARC (F(3, 28) = 67.12, P<0.01) (Fig. 3A and 3B). Fasting increased c-Fos expression in these brain areas except LHA, and the c-Fos expressions were enhanced by SR-57227 (Fig. 3C and D). These enhancements of c-Fos expression induced by SR-57227 were attenuated by ondansetron in both fed and fasting mice (Fig. 3A–3D).

Bottom Line: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior.However, the relative contribution of 5-HT3 receptor remains unclear.Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

View Article: PubMed Central - PubMed

Affiliation: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.

ABSTRACT

Background: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior. However, the relative contribution of 5-HT3 receptor remains unclear. The present study was aimed to investigate the role of 5-HT3 receptor in control of feeding behavior in fed and fasted mice.

Methodology/principal findings: Food intake and expression of c-Fos, tyrosine hydroxylase (TH), proopiomelanocortin (POMC) and 5-HT in the brain were examined after acute treatment with 5-HT3 receptor agonist SR-57227 alone or in combination with 5-HT3 receptor antagonist ondansetron. Food intake was significantly inhibited within 3 h after acute treatment with SR 57227 in fasted mice but not fed mice, and this inhibition was blocked by ondansetron. Immunohistochemical study revealed that fasting-induced c-Fos expression was further enhanced by SR 57227 in the brainstem and the hypothalamus, and this enhancement was also blocked by ondansetron. Furthermore, the fasting-induced downregulation of POMC expression in the hypothalamus and the TH expression in the brain stem was blocked by SR 57227 in the fasted mice, and this effect of SR 57227 was also antagonized by ondansetron.

Conclusion/significance: Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

No MeSH data available.


Related in: MedlinePlus