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Role of 5-HT3 receptor on food intake in fed and fasted mice.

Li B, Shao D, Luo Y, Wang P, Liu C, Zhang X, Cui R - PLoS ONE (2015)

Bottom Line: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior.However, the relative contribution of 5-HT3 receptor remains unclear.Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

View Article: PubMed Central - PubMed

Affiliation: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.

ABSTRACT

Background: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior. However, the relative contribution of 5-HT3 receptor remains unclear. The present study was aimed to investigate the role of 5-HT3 receptor in control of feeding behavior in fed and fasted mice.

Methodology/principal findings: Food intake and expression of c-Fos, tyrosine hydroxylase (TH), proopiomelanocortin (POMC) and 5-HT in the brain were examined after acute treatment with 5-HT3 receptor agonist SR-57227 alone or in combination with 5-HT3 receptor antagonist ondansetron. Food intake was significantly inhibited within 3 h after acute treatment with SR 57227 in fasted mice but not fed mice, and this inhibition was blocked by ondansetron. Immunohistochemical study revealed that fasting-induced c-Fos expression was further enhanced by SR 57227 in the brainstem and the hypothalamus, and this enhancement was also blocked by ondansetron. Furthermore, the fasting-induced downregulation of POMC expression in the hypothalamus and the TH expression in the brain stem was blocked by SR 57227 in the fasted mice, and this effect of SR 57227 was also antagonized by ondansetron.

Conclusion/significance: Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

No MeSH data available.


Related in: MedlinePlus

c-Fos expression in brain stem of fed and fasted mice.Typical photomicrographs of c-Fos expression in DVC of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in DVC of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). DVC: dorsal vagal complex; NTS: nucleus tractus solitarius; DMV: dorsal motor nucleus of the vagus; AP:area postrema; CC: central canal. Values are means ± SEM (n = 7–8). ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; **, P < 0.01; ***, P < 0.001 vs. fasting control group.
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pone.0121473.g002: c-Fos expression in brain stem of fed and fasted mice.Typical photomicrographs of c-Fos expression in DVC of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in DVC of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). DVC: dorsal vagal complex; NTS: nucleus tractus solitarius; DMV: dorsal motor nucleus of the vagus; AP:area postrema; CC: central canal. Values are means ± SEM (n = 7–8). ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; **, P < 0.01; ***, P < 0.001 vs. fasting control group.

Mentions: For the number of c-Fos-positive cells, one way ANOVA revealed that c-Fos expression was significantly increased by SR-57227 (10 mg/kg, i.p) in the DMV (F(3, 28) = 55.82, P<0.01), NTS (F(3, 28) = 88.0, P<0.01) and AP (F(3, 28) = 49, P<0.01) of brain stem in fed mice (Fig. 2A and 2B), and these increases were blocked by ondansetron (Fig. 2A and 2B). Fasting for 18 h significantly increased c-Fos expression in the DMV (F(3, 27) = 94.27, P<0.01), NTS (F(3, 27) = 102.2, P<0.01) and AP (F(3, 27) = 27.25, P<0.01) of brain stem in mice (Fig. 2C and 2D). Furthermore, the fasting-induced upregulation of c-Fos expression was enhanced by SR-57227, and these effects of SR-57227 were blocked by ondansetron (Fig. 2C and 2D).


Role of 5-HT3 receptor on food intake in fed and fasted mice.

Li B, Shao D, Luo Y, Wang P, Liu C, Zhang X, Cui R - PLoS ONE (2015)

c-Fos expression in brain stem of fed and fasted mice.Typical photomicrographs of c-Fos expression in DVC of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in DVC of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). DVC: dorsal vagal complex; NTS: nucleus tractus solitarius; DMV: dorsal motor nucleus of the vagus; AP:area postrema; CC: central canal. Values are means ± SEM (n = 7–8). ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; **, P < 0.01; ***, P < 0.001 vs. fasting control group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4366218&req=5

pone.0121473.g002: c-Fos expression in brain stem of fed and fasted mice.Typical photomicrographs of c-Fos expression in DVC of fed mice (A) and fasted mice (C); Mean number of c-Fos-positive cells in DVC of fed mice (B) and fasted mice (D). SR: SR 57227 (10 mg/kg, i.p.); On: ondansetron (3 mg/kg, i.p.). DVC: dorsal vagal complex; NTS: nucleus tractus solitarius; DMV: dorsal motor nucleus of the vagus; AP:area postrema; CC: central canal. Values are means ± SEM (n = 7–8). ##, P < 0.01; ###, P < 0.001 vs. non-fasting control group; **, P < 0.01; ***, P < 0.001 vs. fasting control group.
Mentions: For the number of c-Fos-positive cells, one way ANOVA revealed that c-Fos expression was significantly increased by SR-57227 (10 mg/kg, i.p) in the DMV (F(3, 28) = 55.82, P<0.01), NTS (F(3, 28) = 88.0, P<0.01) and AP (F(3, 28) = 49, P<0.01) of brain stem in fed mice (Fig. 2A and 2B), and these increases were blocked by ondansetron (Fig. 2A and 2B). Fasting for 18 h significantly increased c-Fos expression in the DMV (F(3, 27) = 94.27, P<0.01), NTS (F(3, 27) = 102.2, P<0.01) and AP (F(3, 27) = 27.25, P<0.01) of brain stem in mice (Fig. 2C and 2D). Furthermore, the fasting-induced upregulation of c-Fos expression was enhanced by SR-57227, and these effects of SR-57227 were blocked by ondansetron (Fig. 2C and 2D).

Bottom Line: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior.However, the relative contribution of 5-HT3 receptor remains unclear.Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

View Article: PubMed Central - PubMed

Affiliation: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.

ABSTRACT

Background: Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior. However, the relative contribution of 5-HT3 receptor remains unclear. The present study was aimed to investigate the role of 5-HT3 receptor in control of feeding behavior in fed and fasted mice.

Methodology/principal findings: Food intake and expression of c-Fos, tyrosine hydroxylase (TH), proopiomelanocortin (POMC) and 5-HT in the brain were examined after acute treatment with 5-HT3 receptor agonist SR-57227 alone or in combination with 5-HT3 receptor antagonist ondansetron. Food intake was significantly inhibited within 3 h after acute treatment with SR 57227 in fasted mice but not fed mice, and this inhibition was blocked by ondansetron. Immunohistochemical study revealed that fasting-induced c-Fos expression was further enhanced by SR 57227 in the brainstem and the hypothalamus, and this enhancement was also blocked by ondansetron. Furthermore, the fasting-induced downregulation of POMC expression in the hypothalamus and the TH expression in the brain stem was blocked by SR 57227 in the fasted mice, and this effect of SR 57227 was also antagonized by ondansetron.

Conclusion/significance: Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.

No MeSH data available.


Related in: MedlinePlus