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Two half-sandwiched ruthenium (II) compounds containing 5-fluorouracil derivatives: synthesis and study of DNA intercalation.

Li ZJ, Hou Y, Qin DA, Jin ZM, Hu ML - PLoS ONE (2015)

Bottom Line: Two novel coordination compounds of half-sandwiched ruthenium(II) containing 2-(5-fluorouracil)-yl-N-(pyridyl)-acetamide were synthesized, and their intercalation binding modes with calf thymus DNA were revealed by hyperchromism of ultraviolet-visible spectroscopy; the binding constants were determined according to a Langmuir adsorption equation that was deduced on the base of careful cyclic voltammetry measurements.The two compounds exhibited DNA intercalation binding activities with the binding constants of 1.13×106 M-1 and 5.35 ×105 M-1, respectively.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, China.

ABSTRACT
Two novel coordination compounds of half-sandwiched ruthenium(II) containing 2-(5-fluorouracil)-yl-N-(pyridyl)-acetamide were synthesized, and their intercalation binding modes with calf thymus DNA were revealed by hyperchromism of ultraviolet-visible spectroscopy; the binding constants were determined according to a Langmuir adsorption equation that was deduced on the base of careful cyclic voltammetry measurements. The two compounds exhibited DNA intercalation binding activities with the binding constants of 1.13×106 M-1 and 5.35 ×105 M-1, respectively.

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The relationship between 1/ΔIP and 1/C.
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pone.0120211.g005: The relationship between 1/ΔIP and 1/C.

Mentions: As equations (1.3) and (1.6) were put into (1.1), it yielded:logΔIΔImax−ΔI=log K+m log[DRUG](1.7)1ΔI=1ΔImax+1ΔImax⋅K⋅1[DRUG]m(1.8)As for Equation (1.8), we assumed m = 1, using ΔIP to represent ΔI, and ΔIp, max to represent ΔImax. As shown in Fig. 5, 1/[DRUG] demonstrated a good linear relationship with 1/ΔIp, indicating reasonable assumptive value of m for compounds I and II. Thus, the equation (1.9) was deduced [24], where ΔIp = Ip-Ipo, Ip and Ipo represent the oxidation peak current of Fe(CN)63-/4- in the presence and absence of the drugs, respectively; ΔIp,max was the maximum difference of the oxidation peak current; and [DRUG] represented the concentration of the drug.


Two half-sandwiched ruthenium (II) compounds containing 5-fluorouracil derivatives: synthesis and study of DNA intercalation.

Li ZJ, Hou Y, Qin DA, Jin ZM, Hu ML - PLoS ONE (2015)

The relationship between 1/ΔIP and 1/C.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4366203&req=5

pone.0120211.g005: The relationship between 1/ΔIP and 1/C.
Mentions: As equations (1.3) and (1.6) were put into (1.1), it yielded:logΔIΔImax−ΔI=log K+m log[DRUG](1.7)1ΔI=1ΔImax+1ΔImax⋅K⋅1[DRUG]m(1.8)As for Equation (1.8), we assumed m = 1, using ΔIP to represent ΔI, and ΔIp, max to represent ΔImax. As shown in Fig. 5, 1/[DRUG] demonstrated a good linear relationship with 1/ΔIp, indicating reasonable assumptive value of m for compounds I and II. Thus, the equation (1.9) was deduced [24], where ΔIp = Ip-Ipo, Ip and Ipo represent the oxidation peak current of Fe(CN)63-/4- in the presence and absence of the drugs, respectively; ΔIp,max was the maximum difference of the oxidation peak current; and [DRUG] represented the concentration of the drug.

Bottom Line: Two novel coordination compounds of half-sandwiched ruthenium(II) containing 2-(5-fluorouracil)-yl-N-(pyridyl)-acetamide were synthesized, and their intercalation binding modes with calf thymus DNA were revealed by hyperchromism of ultraviolet-visible spectroscopy; the binding constants were determined according to a Langmuir adsorption equation that was deduced on the base of careful cyclic voltammetry measurements.The two compounds exhibited DNA intercalation binding activities with the binding constants of 1.13×106 M-1 and 5.35 ×105 M-1, respectively.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, China.

ABSTRACT
Two novel coordination compounds of half-sandwiched ruthenium(II) containing 2-(5-fluorouracil)-yl-N-(pyridyl)-acetamide were synthesized, and their intercalation binding modes with calf thymus DNA were revealed by hyperchromism of ultraviolet-visible spectroscopy; the binding constants were determined according to a Langmuir adsorption equation that was deduced on the base of careful cyclic voltammetry measurements. The two compounds exhibited DNA intercalation binding activities with the binding constants of 1.13×106 M-1 and 5.35 ×105 M-1, respectively.

Show MeSH