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Proteomic study related to vascular connections in watermelon scions grafted onto bottle-gourd rootstock under different light intensities.

Muneer S, Ko CH, Soundararajan P, Manivnnan A, Park YG, Jeong BR - PLoS ONE (2015)

Bottom Line: The enhancement of ion-binding, transcriptional regulation, amino acid metabolism, and defense response proteins suggests a strengthening of the connection between the rootstock and scion under high light intensity.Indeed, the accumulation of key enzymes in the biological processes described above appears to play an important role in the vascular connections of grafted seedlings.Moreover, it appears that 100 μmol m-2 s-1 results in better protein expression responses in grafted seedlings.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660-701, Korea.

ABSTRACT
Although grafting is broadly used in the production of crops, no information is available about the proteins involved in vascular connections between rootstock and scion. Similarly, proteome changes under the light intensities widely used for grafted seedlings are of practical use. The objective of this study was to determine the proteome of vascular connections using watermelon (Citrullus vulgaris Schrad.) 'Sambok Honey' and 'Speed' as the scion and bottle gourd (Lagenaria siceraria Stanld.) 'RS Dongjanggun' as the rootstock grown under different light intensities (25, 50, 75 and 100 μmol m-2 s-1). Our proteomic analysis revealed 24 and 27 differentially expressed proteins in 'Sambok Honey' and 'Speed', respectively, under different light intensities. The identified proteins were largely involved in ion binding, amino acid metabolism, transcriptional regulation and defense response. The enhancement of ion-binding, transcriptional regulation, amino acid metabolism, and defense response proteins suggests a strengthening of the connection between the rootstock and scion under high light intensity. Indeed, the accumulation of key enzymes in the biological processes described above appears to play an important role in the vascular connections of grafted seedlings. Moreover, it appears that 100 μmol m-2 s-1 results in better protein expression responses in grafted seedlings.

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Functional classification of identified proteins from vascular connections (connected portions) in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).(A) ‘Sambok honey’ (B) ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD). The proteins identified were classified based on their putative biological functions.
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pone.0120899.g004: Functional classification of identified proteins from vascular connections (connected portions) in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).(A) ‘Sambok honey’ (B) ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD). The proteins identified were classified based on their putative biological functions.

Mentions: All identified proteins were classified into different categories based on their biological function (www.geneontology.com) (Fig. 4A). Most of the proteins identified in the vascular connections of bottle gourd rootstock-grafted watermelon fell under the categories of ion binding (22%), amino acid biosynthesis (9%), defense response (5%), carbohydrate metabolism (9%), transcriptional regulation (9%), microtubule-based movement (9%), secondary metabolism (13%) and uncharacterized proteins (18%).


Proteomic study related to vascular connections in watermelon scions grafted onto bottle-gourd rootstock under different light intensities.

Muneer S, Ko CH, Soundararajan P, Manivnnan A, Park YG, Jeong BR - PLoS ONE (2015)

Functional classification of identified proteins from vascular connections (connected portions) in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).(A) ‘Sambok honey’ (B) ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD). The proteins identified were classified based on their putative biological functions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4366178&req=5

pone.0120899.g004: Functional classification of identified proteins from vascular connections (connected portions) in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).(A) ‘Sambok honey’ (B) ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD). The proteins identified were classified based on their putative biological functions.
Mentions: All identified proteins were classified into different categories based on their biological function (www.geneontology.com) (Fig. 4A). Most of the proteins identified in the vascular connections of bottle gourd rootstock-grafted watermelon fell under the categories of ion binding (22%), amino acid biosynthesis (9%), defense response (5%), carbohydrate metabolism (9%), transcriptional regulation (9%), microtubule-based movement (9%), secondary metabolism (13%) and uncharacterized proteins (18%).

Bottom Line: The enhancement of ion-binding, transcriptional regulation, amino acid metabolism, and defense response proteins suggests a strengthening of the connection between the rootstock and scion under high light intensity.Indeed, the accumulation of key enzymes in the biological processes described above appears to play an important role in the vascular connections of grafted seedlings.Moreover, it appears that 100 μmol m-2 s-1 results in better protein expression responses in grafted seedlings.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660-701, Korea.

ABSTRACT
Although grafting is broadly used in the production of crops, no information is available about the proteins involved in vascular connections between rootstock and scion. Similarly, proteome changes under the light intensities widely used for grafted seedlings are of practical use. The objective of this study was to determine the proteome of vascular connections using watermelon (Citrullus vulgaris Schrad.) 'Sambok Honey' and 'Speed' as the scion and bottle gourd (Lagenaria siceraria Stanld.) 'RS Dongjanggun' as the rootstock grown under different light intensities (25, 50, 75 and 100 μmol m-2 s-1). Our proteomic analysis revealed 24 and 27 differentially expressed proteins in 'Sambok Honey' and 'Speed', respectively, under different light intensities. The identified proteins were largely involved in ion binding, amino acid metabolism, transcriptional regulation and defense response. The enhancement of ion-binding, transcriptional regulation, amino acid metabolism, and defense response proteins suggests a strengthening of the connection between the rootstock and scion under high light intensity. Indeed, the accumulation of key enzymes in the biological processes described above appears to play an important role in the vascular connections of grafted seedlings. Moreover, it appears that 100 μmol m-2 s-1 results in better protein expression responses in grafted seedlings.

Show MeSH