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Easy to use plastic optical fiber-based biosensor for detection of butanal.

Cennamo N, Di Giovanni S, Varriale A, Staiano M, Di Pietrantonio F, Notargiacomo A, Zeni L, D'Auria S - PLoS ONE (2015)

Bottom Line: The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach.This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform.The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.

View Article: PubMed Central - PubMed

Affiliation: Department of Industrial and Information Engineering, SUN, Aversa, Italy.

ABSTRACT
The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.

No MeSH data available.


Atomic force microscopy.3D rendering of: a gold coated Si substrate a) before and b) after functionalization with lipoic acid, and c) after additional subsequent functionalization with butyric acid and pOBP. Note that a different Z scale was used for the three images.
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pone.0116770.g002: Atomic force microscopy.3D rendering of: a gold coated Si substrate a) before and b) after functionalization with lipoic acid, and c) after additional subsequent functionalization with butyric acid and pOBP. Note that a different Z scale was used for the three images.

Mentions: AFM measurments of the sample topography were performed after each fabrication step. The gold coated Si substrate (Fig. 2a) has a grainy like morphology uniformly covering the surface, showing an Rq (roguhness) value of ~0.85nm. After functionalization with lipoic acid (Fig. 2b) rounded clusters appear with diameter of about 50nm and height of ~8–10nm. The Rq surface roughness at this stage has slightly increased to a value of ~1.2nm. On large area scans, random islands or terraces are also present with size of few hundreds of nanometers. Finally, after additional functionalization with butirric acid and pOBP the morphology exhibits a significant change showing a large amount of aggregates. In particular, two kinds of structures protruding from a flat surface are visible: several microns long filaments (Fig. 2c) with diameter of the order of 50nm, and flat aggregates with micrometric size.


Easy to use plastic optical fiber-based biosensor for detection of butanal.

Cennamo N, Di Giovanni S, Varriale A, Staiano M, Di Pietrantonio F, Notargiacomo A, Zeni L, D'Auria S - PLoS ONE (2015)

Atomic force microscopy.3D rendering of: a gold coated Si substrate a) before and b) after functionalization with lipoic acid, and c) after additional subsequent functionalization with butyric acid and pOBP. Note that a different Z scale was used for the three images.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4366154&req=5

pone.0116770.g002: Atomic force microscopy.3D rendering of: a gold coated Si substrate a) before and b) after functionalization with lipoic acid, and c) after additional subsequent functionalization with butyric acid and pOBP. Note that a different Z scale was used for the three images.
Mentions: AFM measurments of the sample topography were performed after each fabrication step. The gold coated Si substrate (Fig. 2a) has a grainy like morphology uniformly covering the surface, showing an Rq (roguhness) value of ~0.85nm. After functionalization with lipoic acid (Fig. 2b) rounded clusters appear with diameter of about 50nm and height of ~8–10nm. The Rq surface roughness at this stage has slightly increased to a value of ~1.2nm. On large area scans, random islands or terraces are also present with size of few hundreds of nanometers. Finally, after additional functionalization with butirric acid and pOBP the morphology exhibits a significant change showing a large amount of aggregates. In particular, two kinds of structures protruding from a flat surface are visible: several microns long filaments (Fig. 2c) with diameter of the order of 50nm, and flat aggregates with micrometric size.

Bottom Line: The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach.This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform.The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.

View Article: PubMed Central - PubMed

Affiliation: Department of Industrial and Information Engineering, SUN, Aversa, Italy.

ABSTRACT
The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.

No MeSH data available.