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Development of an amperometric-based glucose biosensor to measure the glucose content of fruit.

Ang LF, Por LY, Yam MF - PLoS ONE (2015)

Bottom Line: The optimum experimental conditions for the biosensors in analysing glucose dissolved in 0.1 M phosphate buffer (pH 6.0) were found to be 35°C and 0.6 V applied potential.Their accuracy was compared to that of a commercial glucose assay kit.There was no significance different between two methods, indicating the introduced biosensor is reliable.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.

ABSTRACT
An amperometric enzyme-electrode was introduced where glucose oxidase (GOD) was immobilized on chitosan membrane via crosslinking, and then fastened on a platinum working electrode. The immobilized enzyme showed relatively high retention activity. The activity of the immobilized enzyme was influenced by its loading, being suppressed when more than 0.6 mg enzyme was used in the immobilization. The biosensor showing the highest response to glucose utilized 0.21 ml/cm2 thick chitosan membrane. The optimum experimental conditions for the biosensors in analysing glucose dissolved in 0.1 M phosphate buffer (pH 6.0) were found to be 35°C and 0.6 V applied potential. The introduced biosensor reached a steady-state current at 60 s. The apparent Michaelis-Menten constant ([Formula: see text]) of the biosensor was 14.2350 mM, and its detection limit was 0.05 mM at s/n > 3, determined experimentally. The RSD of repeatability and reproducibility of the biosensor were 2.30% and 3.70%, respectively. The biosensor was showed good stability; it retained ~36% of initial activity after two months of investigation. The performance of the biosensors was evaluated by determining the glucose content in fruit homogenates. Their accuracy was compared to that of a commercial glucose assay kit. There was no significance different between two methods, indicating the introduced biosensor is reliable.

No MeSH data available.


Related in: MedlinePlus

Effect of enzyme concentration used in immobilization on GOD-CHIT/PT response to 4.76 mM glucose.Mean ± SD, n = 6.
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pone.0111859.g004: Effect of enzyme concentration used in immobilization on GOD-CHIT/PT response to 4.76 mM glucose.Mean ± SD, n = 6.

Mentions: 3.2.4 Effect of the enzyme concentration used in immobilization on biosensor response. The amount of the enzyme used in immobilization was varied from 0.05 to 0.80 mg. The effect of enzyme concentration on the response of the GOD-CHIT/PT to 4.76 mM glucose is shown in Fig. 4. The GOD-CHIT/PT response to glucose was relatively low at enzyme below 0.6 mg GOD concentration. Its response increased by about 60% with 0.4–0.6 mg of glucose, where it showed an optimal response. The response plateaued from 0.6–0.8 mg.


Development of an amperometric-based glucose biosensor to measure the glucose content of fruit.

Ang LF, Por LY, Yam MF - PLoS ONE (2015)

Effect of enzyme concentration used in immobilization on GOD-CHIT/PT response to 4.76 mM glucose.Mean ± SD, n = 6.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4366129&req=5

pone.0111859.g004: Effect of enzyme concentration used in immobilization on GOD-CHIT/PT response to 4.76 mM glucose.Mean ± SD, n = 6.
Mentions: 3.2.4 Effect of the enzyme concentration used in immobilization on biosensor response. The amount of the enzyme used in immobilization was varied from 0.05 to 0.80 mg. The effect of enzyme concentration on the response of the GOD-CHIT/PT to 4.76 mM glucose is shown in Fig. 4. The GOD-CHIT/PT response to glucose was relatively low at enzyme below 0.6 mg GOD concentration. Its response increased by about 60% with 0.4–0.6 mg of glucose, where it showed an optimal response. The response plateaued from 0.6–0.8 mg.

Bottom Line: The optimum experimental conditions for the biosensors in analysing glucose dissolved in 0.1 M phosphate buffer (pH 6.0) were found to be 35°C and 0.6 V applied potential.Their accuracy was compared to that of a commercial glucose assay kit.There was no significance different between two methods, indicating the introduced biosensor is reliable.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.

ABSTRACT
An amperometric enzyme-electrode was introduced where glucose oxidase (GOD) was immobilized on chitosan membrane via crosslinking, and then fastened on a platinum working electrode. The immobilized enzyme showed relatively high retention activity. The activity of the immobilized enzyme was influenced by its loading, being suppressed when more than 0.6 mg enzyme was used in the immobilization. The biosensor showing the highest response to glucose utilized 0.21 ml/cm2 thick chitosan membrane. The optimum experimental conditions for the biosensors in analysing glucose dissolved in 0.1 M phosphate buffer (pH 6.0) were found to be 35°C and 0.6 V applied potential. The introduced biosensor reached a steady-state current at 60 s. The apparent Michaelis-Menten constant ([Formula: see text]) of the biosensor was 14.2350 mM, and its detection limit was 0.05 mM at s/n > 3, determined experimentally. The RSD of repeatability and reproducibility of the biosensor were 2.30% and 3.70%, respectively. The biosensor was showed good stability; it retained ~36% of initial activity after two months of investigation. The performance of the biosensors was evaluated by determining the glucose content in fruit homogenates. Their accuracy was compared to that of a commercial glucose assay kit. There was no significance different between two methods, indicating the introduced biosensor is reliable.

No MeSH data available.


Related in: MedlinePlus