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Bone marrow mesenchymal stem cells can differentiate and assume corneal keratocyte phenotype.

Liu H, Zhang J, Liu CY, Hayashi Y, Kao WW - J. Cell. Mol. Med. (2012)

Bottom Line: EGFP(+) BM cells assumed dendritic cell morphology, but failed to synthesize corneal-specific keratan sulfate proteoglycans, that is KS-lumican and KS-keratocan.It is worthy to note that transplanted BM cells were rejected in 4 weeks.These observations suggest that corneal intrastromal transplantation of BM-MSC may be an effective treatment regimen for corneal diseases involving dysfunction of keratocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Edith Crawley Vision Research Center, University of Cincinnati, Cincinnati, OH, USA. liuhs@uc.edu

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The numbers of BM-derived cells decreased with time extension after the transplantation. (A) One week after the injection, a large number of BM-derived cells were distributed in corneal stroma and displayed a variety of cell morphology. (B) Two weeks after the transplantation, green cells displayed dendritic cell shape in corneal stroma; however, the numbers of BM-derived cells obviously decreased. (C) Four weeks after intrastromal injection, only several green cells continuously stayed in the cornea. (D) The number of BM-derived cells was determined by counting green cells in serial Z-stack images and the results revealed that the number of transplanted green cells rapidly decreased and almost all green cells were exhausted 4 weeks after the transplantation. P < 0.001; scale bar: 50 μm; Green: EGFP; Blue: DAPI.
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fig04: The numbers of BM-derived cells decreased with time extension after the transplantation. (A) One week after the injection, a large number of BM-derived cells were distributed in corneal stroma and displayed a variety of cell morphology. (B) Two weeks after the transplantation, green cells displayed dendritic cell shape in corneal stroma; however, the numbers of BM-derived cells obviously decreased. (C) Four weeks after intrastromal injection, only several green cells continuously stayed in the cornea. (D) The number of BM-derived cells was determined by counting green cells in serial Z-stack images and the results revealed that the number of transplanted green cells rapidly decreased and almost all green cells were exhausted 4 weeks after the transplantation. P < 0.001; scale bar: 50 μm; Green: EGFP; Blue: DAPI.

Mentions: To elucidate whether intrastromal transplantation of allogeneic BM cells can be used to heal corneal diseases, a crucial requirement is that BM cells can survive and function in an allogeneic cornea. To address this question, excised Kera−/− mouse cornea that had been treated with EGFP+ BM cell transplantation were stained with DAPI and scanned by a ZEISS Observer.Z1 fluorescent microscope with ApoTome Mode and the number of transplanted cells was determined by counting green cells in serial Z-stack images. The results revealed that the number of transplanted green cells rapidly decreased and almost all green cells vanished within 4 weeks after transplantation (Fig. 4).


Bone marrow mesenchymal stem cells can differentiate and assume corneal keratocyte phenotype.

Liu H, Zhang J, Liu CY, Hayashi Y, Kao WW - J. Cell. Mol. Med. (2012)

The numbers of BM-derived cells decreased with time extension after the transplantation. (A) One week after the injection, a large number of BM-derived cells were distributed in corneal stroma and displayed a variety of cell morphology. (B) Two weeks after the transplantation, green cells displayed dendritic cell shape in corneal stroma; however, the numbers of BM-derived cells obviously decreased. (C) Four weeks after intrastromal injection, only several green cells continuously stayed in the cornea. (D) The number of BM-derived cells was determined by counting green cells in serial Z-stack images and the results revealed that the number of transplanted green cells rapidly decreased and almost all green cells were exhausted 4 weeks after the transplantation. P < 0.001; scale bar: 50 μm; Green: EGFP; Blue: DAPI.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4365890&req=5

fig04: The numbers of BM-derived cells decreased with time extension after the transplantation. (A) One week after the injection, a large number of BM-derived cells were distributed in corneal stroma and displayed a variety of cell morphology. (B) Two weeks after the transplantation, green cells displayed dendritic cell shape in corneal stroma; however, the numbers of BM-derived cells obviously decreased. (C) Four weeks after intrastromal injection, only several green cells continuously stayed in the cornea. (D) The number of BM-derived cells was determined by counting green cells in serial Z-stack images and the results revealed that the number of transplanted green cells rapidly decreased and almost all green cells were exhausted 4 weeks after the transplantation. P < 0.001; scale bar: 50 μm; Green: EGFP; Blue: DAPI.
Mentions: To elucidate whether intrastromal transplantation of allogeneic BM cells can be used to heal corneal diseases, a crucial requirement is that BM cells can survive and function in an allogeneic cornea. To address this question, excised Kera−/− mouse cornea that had been treated with EGFP+ BM cell transplantation were stained with DAPI and scanned by a ZEISS Observer.Z1 fluorescent microscope with ApoTome Mode and the number of transplanted cells was determined by counting green cells in serial Z-stack images. The results revealed that the number of transplanted green cells rapidly decreased and almost all green cells vanished within 4 weeks after transplantation (Fig. 4).

Bottom Line: EGFP(+) BM cells assumed dendritic cell morphology, but failed to synthesize corneal-specific keratan sulfate proteoglycans, that is KS-lumican and KS-keratocan.It is worthy to note that transplanted BM cells were rejected in 4 weeks.These observations suggest that corneal intrastromal transplantation of BM-MSC may be an effective treatment regimen for corneal diseases involving dysfunction of keratocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Edith Crawley Vision Research Center, University of Cincinnati, Cincinnati, OH, USA. liuhs@uc.edu

Show MeSH
Related in: MedlinePlus