Bone marrow mesenchymal stem cells can differentiate and assume corneal keratocyte phenotype.
Bottom Line: EGFP(+) BM cells assumed dendritic cell morphology, but failed to synthesize corneal-specific keratan sulfate proteoglycans, that is KS-lumican and KS-keratocan.It is worthy to note that transplanted BM cells were rejected in 4 weeks.These observations suggest that corneal intrastromal transplantation of BM-MSC may be an effective treatment regimen for corneal diseases involving dysfunction of keratocytes.
Affiliation: Department of Ophthalmology, Edith Crawley Vision Research Center, University of Cincinnati, Cincinnati, OH, USA. email@example.comShow MeSH
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Mentions: To elucidate whether intrastromal transplantation of allogeneic BM cells can be used to heal corneal diseases, a crucial requirement is that BM cells can survive and function in an allogeneic cornea. To address this question, excised Kera−/− mouse cornea that had been treated with EGFP+ BM cell transplantation were stained with DAPI and scanned by a ZEISS Observer.Z1 fluorescent microscope with ApoTome Mode and the number of transplanted cells was determined by counting green cells in serial Z-stack images. The results revealed that the number of transplanted green cells rapidly decreased and almost all green cells vanished within 4 weeks after transplantation (Fig. 4).
Affiliation: Department of Ophthalmology, Edith Crawley Vision Research Center, University of Cincinnati, Cincinnati, OH, USA. firstname.lastname@example.org