miR-145 is differentially regulated by TGF-β1 and ischaemia and targets Disabled-2 expression and wnt/β-catenin activity.
Bottom Line: The effect of wnt/β-catenin signalling in the response to acute myocardial infarction (AMI) remains controversial.There was no significant Dab2 expression in sham-operated myocardium.Our data demonstrate a novel and critical role for miR-145 expression as a regulator of Dab2 expression and β-catenin activity in response to TGF-β(1) and hypoxia.
Affiliation: Skirball Laboratory for Cardiovascular Cellular Therapeutics, Cleveland, OH, USA.Show MeSH
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Mentions: To determine if miR-145 was responsible for the regulation of Dab2 in the heart in response to ischaemia, we quantified pri-miR-145 at 1 hr after LAD ligation. As shown in Figure 6, there is a significant level of pri-miR-145 in the heart at baseline that is rapidly and significantly decreased as early as 1 hr after AMI. These data are consistent with our findings in vitro with MSC and suggest that down-regulation of miR-145 in response to ischaemia results in an up-regulation of Dab2 protein in the heart.
Affiliation: Skirball Laboratory for Cardiovascular Cellular Therapeutics, Cleveland, OH, USA.