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HMGB-1 induces c-kit+ cell microvascular rolling and adhesion via both toll-like receptor-2 and toll-like receptor-4 of endothelial cells.

Furlani D, Donndorf P, Westien I, Ugurlucan M, Pittermann E, Wang W, Li W, Vollmar B, Steinhoff G, Kaminski A, Ma N - J. Cell. Mol. Med. (2012)

Bottom Line: In knockout animals, the fraction of rolling cells did not differ significantly from control levels.TLR-2 (-/-) and Tlr4 (LPS-del) stem cells in WT mice did not show significant reduction in rolling and adhesion compared to WT cells.HMGB-1 mediates c-kit(+) cell recruitment via endothelial TLR-2 and TLR-4.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, Department of Cardiac Surgery, University Rostock, Rostock, Germany.

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Endogenous leukocyte adhesion on cremaster muscle 15 min. after direct HMGB-1 superfusion. (A) Number (n/mm2) of adherent leukocytes in ‘Control’ and ‘HMGB-1’ groups. (B) Quantitative real-time PCR analysis of the pro-inflammatory signals β1-integrin and CD45 of ‘Control’, ‘HMGB-1’, ‘TLR-2ko’ and ‘TLR-4ko’ groups. The average mRNA expression level of β1-intergrin and CD45 and c-kit in ‘Control’ cremasters was arbitrarily given a value of 1 (line) (*P < 0.05 versus ‘Control’).
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fig05: Endogenous leukocyte adhesion on cremaster muscle 15 min. after direct HMGB-1 superfusion. (A) Number (n/mm2) of adherent leukocytes in ‘Control’ and ‘HMGB-1’ groups. (B) Quantitative real-time PCR analysis of the pro-inflammatory signals β1-integrin and CD45 of ‘Control’, ‘HMGB-1’, ‘TLR-2ko’ and ‘TLR-4ko’ groups. The average mRNA expression level of β1-intergrin and CD45 and c-kit in ‘Control’ cremasters was arbitrarily given a value of 1 (line) (*P < 0.05 versus ‘Control’).

Mentions: Given the potential role of HMGB-1 as mediator of inflammatory response, we carefully evaluated the feasible dosage of the chemokine (dose/response test) by intravital fluorescence microscopy (data not shown). After choosing the favourable amount of protein (400 ng/ml), the expression of crucial markers of inflammation such as β1-integrin and leukocyte common marker CD45 was assessed by real-time PCR. HMGB-1 superfusion induced a moderate increase in EL adhesion (Fig. 5A). However, the levels of β1-integrin and CD45 mRNAs did not change significantly in response to HMGB-1 signal (Fig. 5B). In ‘TLR-2ko’ group, expression levels of the two analysed mRNAs were significantly reduced (Fig. 5B).


HMGB-1 induces c-kit+ cell microvascular rolling and adhesion via both toll-like receptor-2 and toll-like receptor-4 of endothelial cells.

Furlani D, Donndorf P, Westien I, Ugurlucan M, Pittermann E, Wang W, Li W, Vollmar B, Steinhoff G, Kaminski A, Ma N - J. Cell. Mol. Med. (2012)

Endogenous leukocyte adhesion on cremaster muscle 15 min. after direct HMGB-1 superfusion. (A) Number (n/mm2) of adherent leukocytes in ‘Control’ and ‘HMGB-1’ groups. (B) Quantitative real-time PCR analysis of the pro-inflammatory signals β1-integrin and CD45 of ‘Control’, ‘HMGB-1’, ‘TLR-2ko’ and ‘TLR-4ko’ groups. The average mRNA expression level of β1-intergrin and CD45 and c-kit in ‘Control’ cremasters was arbitrarily given a value of 1 (line) (*P < 0.05 versus ‘Control’).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4365888&req=5

fig05: Endogenous leukocyte adhesion on cremaster muscle 15 min. after direct HMGB-1 superfusion. (A) Number (n/mm2) of adherent leukocytes in ‘Control’ and ‘HMGB-1’ groups. (B) Quantitative real-time PCR analysis of the pro-inflammatory signals β1-integrin and CD45 of ‘Control’, ‘HMGB-1’, ‘TLR-2ko’ and ‘TLR-4ko’ groups. The average mRNA expression level of β1-intergrin and CD45 and c-kit in ‘Control’ cremasters was arbitrarily given a value of 1 (line) (*P < 0.05 versus ‘Control’).
Mentions: Given the potential role of HMGB-1 as mediator of inflammatory response, we carefully evaluated the feasible dosage of the chemokine (dose/response test) by intravital fluorescence microscopy (data not shown). After choosing the favourable amount of protein (400 ng/ml), the expression of crucial markers of inflammation such as β1-integrin and leukocyte common marker CD45 was assessed by real-time PCR. HMGB-1 superfusion induced a moderate increase in EL adhesion (Fig. 5A). However, the levels of β1-integrin and CD45 mRNAs did not change significantly in response to HMGB-1 signal (Fig. 5B). In ‘TLR-2ko’ group, expression levels of the two analysed mRNAs were significantly reduced (Fig. 5B).

Bottom Line: In knockout animals, the fraction of rolling cells did not differ significantly from control levels.TLR-2 (-/-) and Tlr4 (LPS-del) stem cells in WT mice did not show significant reduction in rolling and adhesion compared to WT cells.HMGB-1 mediates c-kit(+) cell recruitment via endothelial TLR-2 and TLR-4.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, Department of Cardiac Surgery, University Rostock, Rostock, Germany.

Show MeSH
Related in: MedlinePlus