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Root of Polygonum cuspidatum extract reduces progression of diabetes-induced mesangial cell dysfunction via inhibition of platelet-derived growth factor-BB (PDGF-BB) and interaction with its receptor in streptozotocin-induced diabetic rats.

Sohn E, Kim J, Kim CS, Jo K, Lee YM, Kim JS - BMC Complement Altern Med (2014)

Bottom Line: In addition, the binding of PDGF-BB/PDGFR-ß was inhibited by PCE as shown by an in vitro assay.These results suggest that PCE has an inhibitory effect on mesangial proliferation in diabetic renal tissues via the inhibition of the interaction of PDGF-BB with its receptor.PCE may have beneficial effects in preventing the progression of diabetic nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine Based Herbal Drug Development Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), 1672 Yusengdaero, Yuseong-gu, Daejeon 305-811, South Korea. jskim@kiom.re.kr.

ABSTRACT

Background: Platelet-derived growth factor-BB (PDGF-BB) is highly expressed in the renal tissues of patients with diabetic nephropathy, and it plays an important role in the initiation and progression of diabetic nephropathy. The aim of this study was to evaluate the protective effects of root of Polygonum cuspidatum extract (PCE) on early renal glomerular proliferation in streptozotocin (STZ)-induced diabetic rats.

Methods: PCE (100, 350 mg/kg/day) was administered to diabetic rats for 16 weeks. Blood glucose and albuminuria were measured. Renal histology, α-smooth muscle actin (α-SMA), and proliferating cell nuclear antigen (PCNA) expression levels were also examined.

Results: After 16 weeks of treatment with PCE, severe hyperglycemia and albuminuria were observed in the diabetic rats. The expressions levels of α-SMA and PCNA proteins were significantly increased in the glomeruli of the diabetic rats. The expression levels of PDGF-BB and its receptor expressions were greatly increased in the glomeruli of the diabetic rats. However, PCE markedly reduced albuminuria in the diabetic rats. PCE inhibited α-SMA and PCNA up-regulation and ameliorated PDGF-BB and PEGFR-ß protein expression in the diabetic rats. In addition, the binding of PDGF-BB/PDGFR-ß was inhibited by PCE as shown by an in vitro assay.

Conclusions: These results suggest that PCE has an inhibitory effect on mesangial proliferation in diabetic renal tissues via the inhibition of the interaction of PDGF-BB with its receptor. PCE may have beneficial effects in preventing the progression of diabetic nephropathy.

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Related in: MedlinePlus

Inhibitory effects of PCE on the binding of the PDGF-BB ligand with its receptorin vitro. The experiments were performed in triplicate. All data are expressed as the mean ± SEM.
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Fig3: Inhibitory effects of PCE on the binding of the PDGF-BB ligand with its receptorin vitro. The experiments were performed in triplicate. All data are expressed as the mean ± SEM.

Mentions: To investigate whether PCE could inhibit the binding of the PDGF ligand to its receptor, a sandwich ELISA assay was performed. PCE showed inhibition effect on binding of the PDGF ligand to its receptor in a concentration-dependent manner. PCE exhibited an inhibitory effect on the binding of the PDGF-BB ligand to its receptor (IC50 = 0.185 ± 0.14 μg/ml) (Figure 3).Figure 3


Root of Polygonum cuspidatum extract reduces progression of diabetes-induced mesangial cell dysfunction via inhibition of platelet-derived growth factor-BB (PDGF-BB) and interaction with its receptor in streptozotocin-induced diabetic rats.

Sohn E, Kim J, Kim CS, Jo K, Lee YM, Kim JS - BMC Complement Altern Med (2014)

Inhibitory effects of PCE on the binding of the PDGF-BB ligand with its receptorin vitro. The experiments were performed in triplicate. All data are expressed as the mean ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4364577&req=5

Fig3: Inhibitory effects of PCE on the binding of the PDGF-BB ligand with its receptorin vitro. The experiments were performed in triplicate. All data are expressed as the mean ± SEM.
Mentions: To investigate whether PCE could inhibit the binding of the PDGF ligand to its receptor, a sandwich ELISA assay was performed. PCE showed inhibition effect on binding of the PDGF ligand to its receptor in a concentration-dependent manner. PCE exhibited an inhibitory effect on the binding of the PDGF-BB ligand to its receptor (IC50 = 0.185 ± 0.14 μg/ml) (Figure 3).Figure 3

Bottom Line: In addition, the binding of PDGF-BB/PDGFR-ß was inhibited by PCE as shown by an in vitro assay.These results suggest that PCE has an inhibitory effect on mesangial proliferation in diabetic renal tissues via the inhibition of the interaction of PDGF-BB with its receptor.PCE may have beneficial effects in preventing the progression of diabetic nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine Based Herbal Drug Development Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), 1672 Yusengdaero, Yuseong-gu, Daejeon 305-811, South Korea. jskim@kiom.re.kr.

ABSTRACT

Background: Platelet-derived growth factor-BB (PDGF-BB) is highly expressed in the renal tissues of patients with diabetic nephropathy, and it plays an important role in the initiation and progression of diabetic nephropathy. The aim of this study was to evaluate the protective effects of root of Polygonum cuspidatum extract (PCE) on early renal glomerular proliferation in streptozotocin (STZ)-induced diabetic rats.

Methods: PCE (100, 350 mg/kg/day) was administered to diabetic rats for 16 weeks. Blood glucose and albuminuria were measured. Renal histology, α-smooth muscle actin (α-SMA), and proliferating cell nuclear antigen (PCNA) expression levels were also examined.

Results: After 16 weeks of treatment with PCE, severe hyperglycemia and albuminuria were observed in the diabetic rats. The expressions levels of α-SMA and PCNA proteins were significantly increased in the glomeruli of the diabetic rats. The expression levels of PDGF-BB and its receptor expressions were greatly increased in the glomeruli of the diabetic rats. However, PCE markedly reduced albuminuria in the diabetic rats. PCE inhibited α-SMA and PCNA up-regulation and ameliorated PDGF-BB and PEGFR-ß protein expression in the diabetic rats. In addition, the binding of PDGF-BB/PDGFR-ß was inhibited by PCE as shown by an in vitro assay.

Conclusions: These results suggest that PCE has an inhibitory effect on mesangial proliferation in diabetic renal tissues via the inhibition of the interaction of PDGF-BB with its receptor. PCE may have beneficial effects in preventing the progression of diabetic nephropathy.

Show MeSH
Related in: MedlinePlus