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Butter naturally enriched in cis-9, trans-11 CLA prevents hyperinsulinemia and increases both serum HDL cholesterol and triacylglycerol levels in rats.

de Almeida MM, Luquetti SC, Sabarense CM, do Amaral Corrêa JO, dos Reis LG, Santos da Conceição EP, Lisboa PC, de Moura EG, Gameiro J, da Gama MA, Lopes FC, Garcia RM - Lipids Health Dis (2014)

Bottom Line: Furthermore, R-QUICK was lower in HF-Cb than in NF-So group, while no differences in R-QUICK were observed among NF-So, HF-CLAb and HF-So groups.Feeding rats on a high-fat diet containing butter naturally enriched in cis-9, trans-11 CLA prevented hyperinsulinemia and increased HDL cholesterol, which could be associated with higher levels of cis-9, trans-11 CLA, vaccenic acid, oleic acid and lower levels of short and medium-chain saturated fatty acids from butter naturally modified compared to control butter.On the other hand CLA-enriched butter also increased serum triacylglycerol levels, which could be associated with concomitant increases in the content of trans-9 and trans-10 C18:1 isomers in the CLA-enriched butter.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil. raul.garcia@ufjf.edu.br.

ABSTRACT

Background: Evidence from in vitro and animal studies indicates that conjugated linoleic acid (CLA) possesses anti-diabetic properties, which appear to be attributed to cis-9, trans-11 CLA, the major CLA isomer in ruminant fat. However, there is a shortage of studies addressing CLA from natural source. The present study aimed to evaluate the effects of butter naturally enriched in cis-9, trans-11 CLA on parameters related to glucose tolerance, insulin sensitivity and dyslipidemia in rats.

Methods: Forty male Wistar rats were randomly assigned to the following dietary treatments (n=10/group), for 60 days: 1) Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); 2) High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; 3) High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; and 4) High fat-Soybean oil (HF-So): diet containing 24.0% SO. HF-Cb and HF-CLAb diets contained 0.075% and 0.235% of cis-9, trans-11 CLA, respectively.

Results: HF-CLAb-fed rats had lower serum insulin levels at fasting than those fed with the HF-Cb diet, while the PPARγ protein levels in adipose tissue was increased in HF-CLAb-fed rats compared to HF-Cb-fed rats. Furthermore, R-QUICK was lower in HF-Cb than in NF-So group, while no differences in R-QUICK were observed among NF-So, HF-CLAb and HF-So groups. Serum HDL cholesterol levels were higher in HF-CLAb-fed rats than in those fed NF-So, HF-Cb and HF-So diets, as well as higher in NF-So-fed rats than in HF-Cb and HF-So-fed rats. HF-CLAb, HF-Cb and HF-So diets reduced serum LDL cholesterol levels when compared to NF-So, whereas serum triacylglycerol levels were increased in HF-CLAb.

Conclusion: Feeding rats on a high-fat diet containing butter naturally enriched in cis-9, trans-11 CLA prevented hyperinsulinemia and increased HDL cholesterol, which could be associated with higher levels of cis-9, trans-11 CLA, vaccenic acid, oleic acid and lower levels of short and medium-chain saturated fatty acids from butter naturally modified compared to control butter. On the other hand CLA-enriched butter also increased serum triacylglycerol levels, which could be associated with concomitant increases in the content of trans-9 and trans-10 C18:1 isomers in the CLA-enriched butter.

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Related in: MedlinePlus

Effect of control or naturally enriched incis-9,trans-11 CLA butters on body weight. Male Wistar rats fed the following dietary treatments for 60 days: Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; High fat-Soybean oil (HF-So): diet containing 24.0% SO. All data are presented as mean values ± S.E.M (n = 10 rats/group). Statistically significant differences were determined by Anova followed by Newman-Keuls. *p < 0.05, **p <0.01.
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Fig1: Effect of control or naturally enriched incis-9,trans-11 CLA butters on body weight. Male Wistar rats fed the following dietary treatments for 60 days: Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; High fat-Soybean oil (HF-So): diet containing 24.0% SO. All data are presented as mean values ± S.E.M (n = 10 rats/group). Statistically significant differences were determined by Anova followed by Newman-Keuls. *p < 0.05, **p <0.01.

Mentions: The effect of NF-So, HF-Cb, HF-CLAb and HF-So diets on body weight during all experimental period is shown in Figure 1. There were no differences among dietary treatments.Table 1


Butter naturally enriched in cis-9, trans-11 CLA prevents hyperinsulinemia and increases both serum HDL cholesterol and triacylglycerol levels in rats.

de Almeida MM, Luquetti SC, Sabarense CM, do Amaral Corrêa JO, dos Reis LG, Santos da Conceição EP, Lisboa PC, de Moura EG, Gameiro J, da Gama MA, Lopes FC, Garcia RM - Lipids Health Dis (2014)

Effect of control or naturally enriched incis-9,trans-11 CLA butters on body weight. Male Wistar rats fed the following dietary treatments for 60 days: Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; High fat-Soybean oil (HF-So): diet containing 24.0% SO. All data are presented as mean values ± S.E.M (n = 10 rats/group). Statistically significant differences were determined by Anova followed by Newman-Keuls. *p < 0.05, **p <0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4364335&req=5

Fig1: Effect of control or naturally enriched incis-9,trans-11 CLA butters on body weight. Male Wistar rats fed the following dietary treatments for 60 days: Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; High fat-Soybean oil (HF-So): diet containing 24.0% SO. All data are presented as mean values ± S.E.M (n = 10 rats/group). Statistically significant differences were determined by Anova followed by Newman-Keuls. *p < 0.05, **p <0.01.
Mentions: The effect of NF-So, HF-Cb, HF-CLAb and HF-So diets on body weight during all experimental period is shown in Figure 1. There were no differences among dietary treatments.Table 1

Bottom Line: Furthermore, R-QUICK was lower in HF-Cb than in NF-So group, while no differences in R-QUICK were observed among NF-So, HF-CLAb and HF-So groups.Feeding rats on a high-fat diet containing butter naturally enriched in cis-9, trans-11 CLA prevented hyperinsulinemia and increased HDL cholesterol, which could be associated with higher levels of cis-9, trans-11 CLA, vaccenic acid, oleic acid and lower levels of short and medium-chain saturated fatty acids from butter naturally modified compared to control butter.On the other hand CLA-enriched butter also increased serum triacylglycerol levels, which could be associated with concomitant increases in the content of trans-9 and trans-10 C18:1 isomers in the CLA-enriched butter.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil. raul.garcia@ufjf.edu.br.

ABSTRACT

Background: Evidence from in vitro and animal studies indicates that conjugated linoleic acid (CLA) possesses anti-diabetic properties, which appear to be attributed to cis-9, trans-11 CLA, the major CLA isomer in ruminant fat. However, there is a shortage of studies addressing CLA from natural source. The present study aimed to evaluate the effects of butter naturally enriched in cis-9, trans-11 CLA on parameters related to glucose tolerance, insulin sensitivity and dyslipidemia in rats.

Methods: Forty male Wistar rats were randomly assigned to the following dietary treatments (n=10/group), for 60 days: 1) Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); 2) High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; 3) High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; and 4) High fat-Soybean oil (HF-So): diet containing 24.0% SO. HF-Cb and HF-CLAb diets contained 0.075% and 0.235% of cis-9, trans-11 CLA, respectively.

Results: HF-CLAb-fed rats had lower serum insulin levels at fasting than those fed with the HF-Cb diet, while the PPARγ protein levels in adipose tissue was increased in HF-CLAb-fed rats compared to HF-Cb-fed rats. Furthermore, R-QUICK was lower in HF-Cb than in NF-So group, while no differences in R-QUICK were observed among NF-So, HF-CLAb and HF-So groups. Serum HDL cholesterol levels were higher in HF-CLAb-fed rats than in those fed NF-So, HF-Cb and HF-So diets, as well as higher in NF-So-fed rats than in HF-Cb and HF-So-fed rats. HF-CLAb, HF-Cb and HF-So diets reduced serum LDL cholesterol levels when compared to NF-So, whereas serum triacylglycerol levels were increased in HF-CLAb.

Conclusion: Feeding rats on a high-fat diet containing butter naturally enriched in cis-9, trans-11 CLA prevented hyperinsulinemia and increased HDL cholesterol, which could be associated with higher levels of cis-9, trans-11 CLA, vaccenic acid, oleic acid and lower levels of short and medium-chain saturated fatty acids from butter naturally modified compared to control butter. On the other hand CLA-enriched butter also increased serum triacylglycerol levels, which could be associated with concomitant increases in the content of trans-9 and trans-10 C18:1 isomers in the CLA-enriched butter.

Show MeSH
Related in: MedlinePlus