Limits...
Evaluation of Antiradical and Anti-Inflammatory Activities of Ethyl Acetate and Butanolic Subfractions of Agelanthus dodoneifolius (DC.) Polhill & Wiens (Loranthaceae) Using Equine Myeloperoxidase and Both PMA-Activated Neutrophils and HL-60 Cells.

Boly R, Franck T, Kohnen S, Lompo M, Guissou IP, Dubois J, Serteyn D, Mouithys-Mickalad A - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC50 = 0.3 ± 0.1 µg/mL) and the ELISA and SIEFED assays (IC50 = 2.8 ± 1.2 µg/mL and 1.3 ± 1.0 µg/mL), respectively.However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%.Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds.

View Article: PubMed Central - PubMed

Affiliation: Institute for Research in Health Sciences (IRSS/CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso.

ABSTRACT
The ethyl acetate and n-butanolic subfractions of Agelanthus dodoneifolius were investigated for their antioxidant and antimyeloperoxidase (MPO) activities. The reactive oxygen species (ROS) generation was assessed by lucigenin-enhanced chemiluminescence (CL) and dichlorofluorescein- (DCF-) induced fluorescence techniques from phorbol myristate acetate- (PMA-) stimulated equine neutrophils and human myeloid cell line HL-60, respectively. In parallel, the effects of the tested subfractions were evaluated on the total MPO release by stimulated neutrophils and on the specific MPO activity by means of immunological assays. The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC50 = 0.3 ± 0.1 µg/mL) and the ELISA and SIEFED assays (IC50 = 2.8 ± 1.2 µg/mL and 1.3 ± 1.0 µg/mL), respectively. However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%. Moreover, we demonstrated that the ethyl acetate subfraction was rich in catechin (16.51%) while it was not easy to identify the main compounds in the butanolic subfraction using the UPLC-MS/MS technique. Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds.

No MeSH data available.


Related in: MedlinePlus

Dichlorofluorescin (DCF) assay. Effect of Agelanthus dodoneifolius subfractions on DCF production in PMA-treated HL-60 cells. Data are expressed as mean ± SD of two parallel experiments. ***P < 0.0001 or *P < 0.01 indicate significance compared to control solutions (one-way ANOVA followed by Dunnett's posttest). NA: not activated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4363892&req=5

fig4: Dichlorofluorescin (DCF) assay. Effect of Agelanthus dodoneifolius subfractions on DCF production in PMA-treated HL-60 cells. Data are expressed as mean ± SD of two parallel experiments. ***P < 0.0001 or *P < 0.01 indicate significance compared to control solutions (one-way ANOVA followed by Dunnett's posttest). NA: not activated cells.

Mentions: Interestingly, the ethyl acetate subfraction was more potent at inhibiting the ROS-induced fluorescence from PMA-treated HL-60 cells while the butanolic one failed as shown in Figure 4.


Evaluation of Antiradical and Anti-Inflammatory Activities of Ethyl Acetate and Butanolic Subfractions of Agelanthus dodoneifolius (DC.) Polhill & Wiens (Loranthaceae) Using Equine Myeloperoxidase and Both PMA-Activated Neutrophils and HL-60 Cells.

Boly R, Franck T, Kohnen S, Lompo M, Guissou IP, Dubois J, Serteyn D, Mouithys-Mickalad A - Evid Based Complement Alternat Med (2015)

Dichlorofluorescin (DCF) assay. Effect of Agelanthus dodoneifolius subfractions on DCF production in PMA-treated HL-60 cells. Data are expressed as mean ± SD of two parallel experiments. ***P < 0.0001 or *P < 0.01 indicate significance compared to control solutions (one-way ANOVA followed by Dunnett's posttest). NA: not activated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4363892&req=5

fig4: Dichlorofluorescin (DCF) assay. Effect of Agelanthus dodoneifolius subfractions on DCF production in PMA-treated HL-60 cells. Data are expressed as mean ± SD of two parallel experiments. ***P < 0.0001 or *P < 0.01 indicate significance compared to control solutions (one-way ANOVA followed by Dunnett's posttest). NA: not activated cells.
Mentions: Interestingly, the ethyl acetate subfraction was more potent at inhibiting the ROS-induced fluorescence from PMA-treated HL-60 cells while the butanolic one failed as shown in Figure 4.

Bottom Line: The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC50 = 0.3 ± 0.1 µg/mL) and the ELISA and SIEFED assays (IC50 = 2.8 ± 1.2 µg/mL and 1.3 ± 1.0 µg/mL), respectively.However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%.Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds.

View Article: PubMed Central - PubMed

Affiliation: Institute for Research in Health Sciences (IRSS/CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso.

ABSTRACT
The ethyl acetate and n-butanolic subfractions of Agelanthus dodoneifolius were investigated for their antioxidant and antimyeloperoxidase (MPO) activities. The reactive oxygen species (ROS) generation was assessed by lucigenin-enhanced chemiluminescence (CL) and dichlorofluorescein- (DCF-) induced fluorescence techniques from phorbol myristate acetate- (PMA-) stimulated equine neutrophils and human myeloid cell line HL-60, respectively. In parallel, the effects of the tested subfractions were evaluated on the total MPO release by stimulated neutrophils and on the specific MPO activity by means of immunological assays. The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC50 = 0.3 ± 0.1 µg/mL) and the ELISA and SIEFED assays (IC50 = 2.8 ± 1.2 µg/mL and 1.3 ± 1.0 µg/mL), respectively. However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%. Moreover, we demonstrated that the ethyl acetate subfraction was rich in catechin (16.51%) while it was not easy to identify the main compounds in the butanolic subfraction using the UPLC-MS/MS technique. Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds.

No MeSH data available.


Related in: MedlinePlus