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Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta.

Cheng D, Lu Y, Zeng L, Liang G, He X - Sci Rep (2015)

Bottom Line: Pathway enrichment analysis revealed that 4 pathways associated with larval development were significantly enriched.Significantly higher expression of these genes was found in 4th-instar larvae, and Pearson correlation analysis of the expression patterns revealed significant relationships among Si-CSP9, PKA, FAAH, and FAT1-4.Moreover, we confirmed that expression levels of Si-CSP9, FAAH, and FAT1-4 were significantly reduced and that the development of 3rd-instar larvae was halted with PKA silencing.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, South China Agricultural University, Guangzhou, Guangdong, People's Republic China.

ABSTRACT
Chemosensory proteins (CSPs) have been predicted to be involved in development; however, direct evidence for their involvement is lacking, and genetic basis is largely unknown. To determine the function of the chemosensory protein 9 (Si-CSP9) gene in Solenopsis invicta, we used RNA interference to silence Si-CSP9 in 3rd-instar larvae. The 3rd-instar larvae failed to shed their cuticle after being fed Si-CSP9-directed siRNA, and expression profiling of RNAi-treated and untreated control larvae showed that 375 genes were differentially expressed. Pathway enrichment analysis revealed that 4 pathways associated with larval development were significantly enriched. Blast analysis revealed that one fatty acid amide hydrolase (FAAH) gene was up-regulated and 4 fatty acid synthase (FAT) genes and one protein kinase DC2 gene (PKA) were down-regulated in the enriched pathways. Significantly higher expression of these genes was found in 4th-instar larvae, and Pearson correlation analysis of the expression patterns revealed significant relationships among Si-CSP9, PKA, FAAH, and FAT1-4. Moreover, we confirmed that expression levels of Si-CSP9, FAAH, and FAT1-4 were significantly reduced and that the development of 3rd-instar larvae was halted with PKA silencing. These results suggest that Si-CSP9 and PKA may be involved in the network that contributes to development of 3rd-instar larvae.

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Development-specific expressions of FAT1-4.Means ± SE that are labelled with the same letter within eachtreatment are not significantly different.
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f9: Development-specific expressions of FAT1-4.Means ± SE that are labelled with the same letter within eachtreatment are not significantly different.

Mentions: A one-way analysis of variance showed that PKA, FAAH, andFAT1-4 had significantly higher expression in 4th-instar larvae thanother developmental stages, identical to Si-CSP9 (PKA: F = 461.68, P < 0.001, Figure 7; FAAH:F = 719.24, P < 0.001, Figure 8;FAT1: F = 1001, P < 0.001; FAT2: F = 2127, P < 0.001; FAT3: F = 2701, P < 0.001;FAT4: F = 1222, P < 0.001, Figure9). The expression pattern correlation analysis between PKA,FAAH, FAT1-4, and Si-CSP9 revealed the same expressionpatterns in the developmental stages, with each gene exhibiting a significantlyrelated Pearson correlation with another gene at the 0.01 level (Table 3).


Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta.

Cheng D, Lu Y, Zeng L, Liang G, He X - Sci Rep (2015)

Development-specific expressions of FAT1-4.Means ± SE that are labelled with the same letter within eachtreatment are not significantly different.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363891&req=5

f9: Development-specific expressions of FAT1-4.Means ± SE that are labelled with the same letter within eachtreatment are not significantly different.
Mentions: A one-way analysis of variance showed that PKA, FAAH, andFAT1-4 had significantly higher expression in 4th-instar larvae thanother developmental stages, identical to Si-CSP9 (PKA: F = 461.68, P < 0.001, Figure 7; FAAH:F = 719.24, P < 0.001, Figure 8;FAT1: F = 1001, P < 0.001; FAT2: F = 2127, P < 0.001; FAT3: F = 2701, P < 0.001;FAT4: F = 1222, P < 0.001, Figure9). The expression pattern correlation analysis between PKA,FAAH, FAT1-4, and Si-CSP9 revealed the same expressionpatterns in the developmental stages, with each gene exhibiting a significantlyrelated Pearson correlation with another gene at the 0.01 level (Table 3).

Bottom Line: Pathway enrichment analysis revealed that 4 pathways associated with larval development were significantly enriched.Significantly higher expression of these genes was found in 4th-instar larvae, and Pearson correlation analysis of the expression patterns revealed significant relationships among Si-CSP9, PKA, FAAH, and FAT1-4.Moreover, we confirmed that expression levels of Si-CSP9, FAAH, and FAT1-4 were significantly reduced and that the development of 3rd-instar larvae was halted with PKA silencing.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, South China Agricultural University, Guangzhou, Guangdong, People's Republic China.

ABSTRACT
Chemosensory proteins (CSPs) have been predicted to be involved in development; however, direct evidence for their involvement is lacking, and genetic basis is largely unknown. To determine the function of the chemosensory protein 9 (Si-CSP9) gene in Solenopsis invicta, we used RNA interference to silence Si-CSP9 in 3rd-instar larvae. The 3rd-instar larvae failed to shed their cuticle after being fed Si-CSP9-directed siRNA, and expression profiling of RNAi-treated and untreated control larvae showed that 375 genes were differentially expressed. Pathway enrichment analysis revealed that 4 pathways associated with larval development were significantly enriched. Blast analysis revealed that one fatty acid amide hydrolase (FAAH) gene was up-regulated and 4 fatty acid synthase (FAT) genes and one protein kinase DC2 gene (PKA) were down-regulated in the enriched pathways. Significantly higher expression of these genes was found in 4th-instar larvae, and Pearson correlation analysis of the expression patterns revealed significant relationships among Si-CSP9, PKA, FAAH, and FAT1-4. Moreover, we confirmed that expression levels of Si-CSP9, FAAH, and FAT1-4 were significantly reduced and that the development of 3rd-instar larvae was halted with PKA silencing. These results suggest that Si-CSP9 and PKA may be involved in the network that contributes to development of 3rd-instar larvae.

Show MeSH
Related in: MedlinePlus