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Quantitative analysis of retinal layers' optical intensities on 3D optical coherence tomography for central retinal artery occlusion.

Chen H, Chen X, Qiu Z, Xiang D, Chen W, Shi F, Zheng J, Zhu W, Sonka M - Sci Rep (2015)

Bottom Line: Layer-specific mean intensities were determined and compared between the patient and control groups using multiple regression analysis while adjusting for age and optical intensity of the entire region.Optical intensities were lower at the photoreceptor, retinal pigment epithelium (RPE), and choroid layers (standardized beta = -0.412 to -0.611, all p < 0.01), possibly due to shadowing effects.Among the intraretinal layers, the inner nuclear layer was identified as the best indicator of CRAO.

View Article: PubMed Central - PubMed

Affiliation: Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.

ABSTRACT
Optical coherence tomography (OCT) provides not only morphological information but also information about layer-specific optical intensities, which may represent the underlying tissue properties. The purpose of this study is to quantitatively investigate the optical intensity of each retinal layers in central retinal artery occlusion (CRAO). Twenty-nine CRAO cases at acute phase and 33 normal controls were included. Macula-centered 3D OCT images were segmented with a fully-automated Iowa Reference Algorithm into 10 layers. Layer-specific mean intensities were determined and compared between the patient and control groups using multiple regression analysis while adjusting for age and optical intensity of the entire region. The optical intensities were higher in CRAO than in controls in layers spanning from the retinal ganglion cell layer to outer plexiform layer (standardized beta = 0.657 to 0.777, all p < 0.001), possibly due to ischemia. Optical intensities were lower at the photoreceptor, retinal pigment epithelium (RPE), and choroid layers (standardized beta = -0.412 to -0.611, all p < 0.01), possibly due to shadowing effects. Among the intraretinal layers, the inner nuclear layer was identified as the best indicator of CRAO. Our study provides in vivo information of the optical intensity changes in each retinal layer in CRAO patients.

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Related in: MedlinePlus

Regression of optical intensities in each layer with intensities of the entire retinal region in retinal artery occlusion patients and controls.Circles represent patients with central retinal artery occlusion, black dots represent control subjects.
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f2: Regression of optical intensities in each layer with intensities of the entire retinal region in retinal artery occlusion patients and controls.Circles represent patients with central retinal artery occlusion, black dots represent control subjects.

Mentions: The mean and standard deviation of optical intensities and optical intensity ratios in each layer are shown in Table 2. Results of multi-linear regression after adjusting for optical intensity of the entire region and age are given in Table 2 and Figure 2. There was no statistically significant difference of optical intensities between the CRAO and control subjects in the vitreous and RNFL (standardized beta = 0.160 and 0.050 respectively, both p > 0.5, Table 2 and Figure 2A.B.). Optical intensities in GCL, IPL, INL and OPL were higher in CRAO compared to controls (standardized beta = 0.657, 0.702, 0.777 and 0.694, respectively, all p < 0.001, Table 2 and Figure 2 C.D.E.F). Optical intensity at ONL + HFL was not different between the CRAO and control groups (standardized beta = 0.047, p > 0.5, Table 2 and Figure 2 G.). Optical intensities at the photoreceptor, RPE, and choroidal layers were lower in the CRAO cases compared to controls (standardized beta = −0.412, −0.611 and −0.559, all p < 0.001, Table 2 and Figure 2 H.I.J.). Discriminant analysis found that the optical intensity of INL was most strongly associated with the CRAO disease status (Wilks' Lambda = 0.641).


Quantitative analysis of retinal layers' optical intensities on 3D optical coherence tomography for central retinal artery occlusion.

Chen H, Chen X, Qiu Z, Xiang D, Chen W, Shi F, Zheng J, Zhu W, Sonka M - Sci Rep (2015)

Regression of optical intensities in each layer with intensities of the entire retinal region in retinal artery occlusion patients and controls.Circles represent patients with central retinal artery occlusion, black dots represent control subjects.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363859&req=5

f2: Regression of optical intensities in each layer with intensities of the entire retinal region in retinal artery occlusion patients and controls.Circles represent patients with central retinal artery occlusion, black dots represent control subjects.
Mentions: The mean and standard deviation of optical intensities and optical intensity ratios in each layer are shown in Table 2. Results of multi-linear regression after adjusting for optical intensity of the entire region and age are given in Table 2 and Figure 2. There was no statistically significant difference of optical intensities between the CRAO and control subjects in the vitreous and RNFL (standardized beta = 0.160 and 0.050 respectively, both p > 0.5, Table 2 and Figure 2A.B.). Optical intensities in GCL, IPL, INL and OPL were higher in CRAO compared to controls (standardized beta = 0.657, 0.702, 0.777 and 0.694, respectively, all p < 0.001, Table 2 and Figure 2 C.D.E.F). Optical intensity at ONL + HFL was not different between the CRAO and control groups (standardized beta = 0.047, p > 0.5, Table 2 and Figure 2 G.). Optical intensities at the photoreceptor, RPE, and choroidal layers were lower in the CRAO cases compared to controls (standardized beta = −0.412, −0.611 and −0.559, all p < 0.001, Table 2 and Figure 2 H.I.J.). Discriminant analysis found that the optical intensity of INL was most strongly associated with the CRAO disease status (Wilks' Lambda = 0.641).

Bottom Line: Layer-specific mean intensities were determined and compared between the patient and control groups using multiple regression analysis while adjusting for age and optical intensity of the entire region.Optical intensities were lower at the photoreceptor, retinal pigment epithelium (RPE), and choroid layers (standardized beta = -0.412 to -0.611, all p < 0.01), possibly due to shadowing effects.Among the intraretinal layers, the inner nuclear layer was identified as the best indicator of CRAO.

View Article: PubMed Central - PubMed

Affiliation: Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.

ABSTRACT
Optical coherence tomography (OCT) provides not only morphological information but also information about layer-specific optical intensities, which may represent the underlying tissue properties. The purpose of this study is to quantitatively investigate the optical intensity of each retinal layers in central retinal artery occlusion (CRAO). Twenty-nine CRAO cases at acute phase and 33 normal controls were included. Macula-centered 3D OCT images were segmented with a fully-automated Iowa Reference Algorithm into 10 layers. Layer-specific mean intensities were determined and compared between the patient and control groups using multiple regression analysis while adjusting for age and optical intensity of the entire region. The optical intensities were higher in CRAO than in controls in layers spanning from the retinal ganglion cell layer to outer plexiform layer (standardized beta = 0.657 to 0.777, all p < 0.001), possibly due to ischemia. Optical intensities were lower at the photoreceptor, retinal pigment epithelium (RPE), and choroid layers (standardized beta = -0.412 to -0.611, all p < 0.01), possibly due to shadowing effects. Among the intraretinal layers, the inner nuclear layer was identified as the best indicator of CRAO. Our study provides in vivo information of the optical intensity changes in each retinal layer in CRAO patients.

Show MeSH
Related in: MedlinePlus