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Retinal Neurodegeneration in db/db Mice at the Early Period of Diabetes.

Yang Q, Xu Y, Xie P, Cheng H, Song Q, Su T, Yuan S, Liu Q - J Ophthalmol (2015)

Bottom Line: In addition, levels of retinal ganglion cell death were measured by terminal dUTP nick-end labeling (TUNEL).Results.IBA-1 and F4/80 expression in microglia/macrophages became evidently for 24-week period, thus supporting the PERG findings.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.

ABSTRACT
Purpose. To describe both the functional and pathological alternations in neurosensory retina in a murine model of spontaneous type 2 diabetes (db/db mouse). Methods. db/db (BKS/DB-/-) mice and heterozygous littermates (as control group) at various ages (12, 16, 20, 24, and 28 weeks) were inspected with pattern electroretinogram (PERG), fundus fluorescein angiography (FFA), and optical coherence tomography (OCT). Histological markers of neuroinflammation (IBA-1 and F4/80) were evaluated by immunohistochemistry. In addition, levels of retinal ganglion cell death were measured by terminal dUTP nick-end labeling (TUNEL). Results. Significant alternations of PERG responses and increased retinal ganglion cells (RGCs) apoptosis were observed in diabetic db/db mice for 20-week period when compared with control group. IBA-1 and F4/80 expression in microglia/macrophages became evidently for 24-week period, thus supporting the PERG findings. Furthermore, obvious thinning of nasal and dorsal retina in 28-week-old db/db mice was also revealed by OCT. No visible retinal microvascular changes were detected by FFA throughout the experiments on db/db mice. Conclusions. Diabetic retina underwent neurodegenerative changes in db/db mice, which happened at retinal ganglion cell layer and inner nuclear layer. But there was no obvious abnormality in retinal vasculature on db/db mice.

No MeSH data available.


Related in: MedlinePlus

No evident retinal vascular changes were observed in db/db mice by FFA. FFA image in db/db mice (a) and db/+ mice (b) at 28 weeks. Neither of them presented abnormal alterations on retinal vascular structures.
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fig4: No evident retinal vascular changes were observed in db/db mice by FFA. FFA image in db/db mice (a) and db/+ mice (b) at 28 weeks. Neither of them presented abnormal alterations on retinal vascular structures.

Mentions: After examinations of PERG, FFA were conducted which monitor the flow of a fluorescent dye through the retinal vasculature. All db/db as well as control mice did not show any alternations corresponding to relevant vascular abnormalities at each endpoint. Figure 4 shows the images of FFA from db/db and db/+ mice at 28 weeks and there was no visible neovascularization and cellular capillaries in the retina.


Retinal Neurodegeneration in db/db Mice at the Early Period of Diabetes.

Yang Q, Xu Y, Xie P, Cheng H, Song Q, Su T, Yuan S, Liu Q - J Ophthalmol (2015)

No evident retinal vascular changes were observed in db/db mice by FFA. FFA image in db/db mice (a) and db/+ mice (b) at 28 weeks. Neither of them presented abnormal alterations on retinal vascular structures.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4363796&req=5

fig4: No evident retinal vascular changes were observed in db/db mice by FFA. FFA image in db/db mice (a) and db/+ mice (b) at 28 weeks. Neither of them presented abnormal alterations on retinal vascular structures.
Mentions: After examinations of PERG, FFA were conducted which monitor the flow of a fluorescent dye through the retinal vasculature. All db/db as well as control mice did not show any alternations corresponding to relevant vascular abnormalities at each endpoint. Figure 4 shows the images of FFA from db/db and db/+ mice at 28 weeks and there was no visible neovascularization and cellular capillaries in the retina.

Bottom Line: In addition, levels of retinal ganglion cell death were measured by terminal dUTP nick-end labeling (TUNEL).Results.IBA-1 and F4/80 expression in microglia/macrophages became evidently for 24-week period, thus supporting the PERG findings.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.

ABSTRACT
Purpose. To describe both the functional and pathological alternations in neurosensory retina in a murine model of spontaneous type 2 diabetes (db/db mouse). Methods. db/db (BKS/DB-/-) mice and heterozygous littermates (as control group) at various ages (12, 16, 20, 24, and 28 weeks) were inspected with pattern electroretinogram (PERG), fundus fluorescein angiography (FFA), and optical coherence tomography (OCT). Histological markers of neuroinflammation (IBA-1 and F4/80) were evaluated by immunohistochemistry. In addition, levels of retinal ganglion cell death were measured by terminal dUTP nick-end labeling (TUNEL). Results. Significant alternations of PERG responses and increased retinal ganglion cells (RGCs) apoptosis were observed in diabetic db/db mice for 20-week period when compared with control group. IBA-1 and F4/80 expression in microglia/macrophages became evidently for 24-week period, thus supporting the PERG findings. Furthermore, obvious thinning of nasal and dorsal retina in 28-week-old db/db mice was also revealed by OCT. No visible retinal microvascular changes were detected by FFA throughout the experiments on db/db mice. Conclusions. Diabetic retina underwent neurodegenerative changes in db/db mice, which happened at retinal ganglion cell layer and inner nuclear layer. But there was no obvious abnormality in retinal vasculature on db/db mice.

No MeSH data available.


Related in: MedlinePlus