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Antinociceptive Effect of the Essential Oil Obtained from the Leaves of Croton cordiifolius Baill. (Euphorbiaceae) in Mice.

Nogueira Lde M, da Silva MR, Dos Santos SM, de Albuquerque JF, Ferraz IC, de Albuquerque TT, Mota CR, Araújo RM, Viana GS, Martins RD, Havt A, Ximenes RM - Evid Based Complement Alternat Med (2015)

Bottom Line: Regarding antinociceptive activity, CcEO (50 and 100 mg/kg) reduced the number of writhing responses induced by acetic acid and decreased the licking times in both phases of the formalin test.Naloxone, an opioid antagonist, did not affect the antinociceptive activity of CcEO in writhing test.In conclusion, the antinociceptive effect of CcEO could be explained, at least in part, by inhibition of the glutamatergic system.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Croton cordiifolius Baill. is a shrub known as "quebra-faca" and is used to treat inflammation, pain, wounds, and gastrointestinal disturbances in the semiarid region in the northeast of Brazil. In an ethnobotanical survey in the state of Pernambuco, "quebra-faca" use was cited in 33% of the interviews. Thus, we decided to evaluate the antinociceptive effects of the essential oil from C. cordiifolius (CcEO). Chemical analysis by gas chromatography-mass spectrometry revealed 1,8-cineole (25.09%) and α-phellandrene (15.43%) as major constituents. Antinociceptive activity was evaluated using murine models of chemically induced pain (writhing induced by acetic acid, formalin, capsaicin, and glutamate tests). Opioid and central nervous systems (CNS) involvement were also investigated. Regarding antinociceptive activity, CcEO (50 and 100 mg/kg) reduced the number of writhing responses induced by acetic acid and decreased the licking times in both phases of the formalin test. CcEO also was evaluated in capsaicin- and glutamate-induced nociception. While no effect was observed in the capsaicin test, CcEO (100 mg/kg) was effective in the glutamate test. Naloxone, an opioid antagonist, did not affect the antinociceptive activity of CcEO in writhing test. In conclusion, the antinociceptive effect of CcEO could be explained, at least in part, by inhibition of the glutamatergic system.

No MeSH data available.


Related in: MedlinePlus

Effect of C. cordiifolius essential oil (CcEO; 50 and 100 mg/kg, i.p.) and indomethacin (INDO; 10 mg/kg, i.p.) in the acetic acid induced writhing test. Results are expressed as mean ± SEM (n = 8) with significance level set at P < 0.05. *When compared to the vehicle group; awhen compared to CcEO 50 mg/kg group.
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Related In: Results  -  Collection


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fig1: Effect of C. cordiifolius essential oil (CcEO; 50 and 100 mg/kg, i.p.) and indomethacin (INDO; 10 mg/kg, i.p.) in the acetic acid induced writhing test. Results are expressed as mean ± SEM (n = 8) with significance level set at P < 0.05. *When compared to the vehicle group; awhen compared to CcEO 50 mg/kg group.

Mentions: In the acetic acid induced writhing, both doses of CcEO reduced the number of writhing in a dose-dependent manner compared to the control group, as showed in Figure 1. This test is a useful tool to evaluate both central and peripheral analgesic activity of new compounds. Acetic acid induces peritoneal resident cells (macrophages and mast cells) to liberate TNF-α and prostaglandin E2, which produce the noxious stimuli. Anti-inflammatory drugs as indomethacin, a cyclooxygenase inhibitor, are effective in reducing pain [27]. Despite the test being sensitive to weak analgesics, it is also sensitive to other pharmacological agents, such as neuroleptics, anticholinergics, and antihistamines [28]. Both CcEO major compounds 1,8-cineole and α-phellandrene, when administrated alone, have been shown to be effective in this animal model [25, 26].


Antinociceptive Effect of the Essential Oil Obtained from the Leaves of Croton cordiifolius Baill. (Euphorbiaceae) in Mice.

Nogueira Lde M, da Silva MR, Dos Santos SM, de Albuquerque JF, Ferraz IC, de Albuquerque TT, Mota CR, Araújo RM, Viana GS, Martins RD, Havt A, Ximenes RM - Evid Based Complement Alternat Med (2015)

Effect of C. cordiifolius essential oil (CcEO; 50 and 100 mg/kg, i.p.) and indomethacin (INDO; 10 mg/kg, i.p.) in the acetic acid induced writhing test. Results are expressed as mean ± SEM (n = 8) with significance level set at P < 0.05. *When compared to the vehicle group; awhen compared to CcEO 50 mg/kg group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4363708&req=5

fig1: Effect of C. cordiifolius essential oil (CcEO; 50 and 100 mg/kg, i.p.) and indomethacin (INDO; 10 mg/kg, i.p.) in the acetic acid induced writhing test. Results are expressed as mean ± SEM (n = 8) with significance level set at P < 0.05. *When compared to the vehicle group; awhen compared to CcEO 50 mg/kg group.
Mentions: In the acetic acid induced writhing, both doses of CcEO reduced the number of writhing in a dose-dependent manner compared to the control group, as showed in Figure 1. This test is a useful tool to evaluate both central and peripheral analgesic activity of new compounds. Acetic acid induces peritoneal resident cells (macrophages and mast cells) to liberate TNF-α and prostaglandin E2, which produce the noxious stimuli. Anti-inflammatory drugs as indomethacin, a cyclooxygenase inhibitor, are effective in reducing pain [27]. Despite the test being sensitive to weak analgesics, it is also sensitive to other pharmacological agents, such as neuroleptics, anticholinergics, and antihistamines [28]. Both CcEO major compounds 1,8-cineole and α-phellandrene, when administrated alone, have been shown to be effective in this animal model [25, 26].

Bottom Line: Regarding antinociceptive activity, CcEO (50 and 100 mg/kg) reduced the number of writhing responses induced by acetic acid and decreased the licking times in both phases of the formalin test.Naloxone, an opioid antagonist, did not affect the antinociceptive activity of CcEO in writhing test.In conclusion, the antinociceptive effect of CcEO could be explained, at least in part, by inhibition of the glutamatergic system.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Croton cordiifolius Baill. is a shrub known as "quebra-faca" and is used to treat inflammation, pain, wounds, and gastrointestinal disturbances in the semiarid region in the northeast of Brazil. In an ethnobotanical survey in the state of Pernambuco, "quebra-faca" use was cited in 33% of the interviews. Thus, we decided to evaluate the antinociceptive effects of the essential oil from C. cordiifolius (CcEO). Chemical analysis by gas chromatography-mass spectrometry revealed 1,8-cineole (25.09%) and α-phellandrene (15.43%) as major constituents. Antinociceptive activity was evaluated using murine models of chemically induced pain (writhing induced by acetic acid, formalin, capsaicin, and glutamate tests). Opioid and central nervous systems (CNS) involvement were also investigated. Regarding antinociceptive activity, CcEO (50 and 100 mg/kg) reduced the number of writhing responses induced by acetic acid and decreased the licking times in both phases of the formalin test. CcEO also was evaluated in capsaicin- and glutamate-induced nociception. While no effect was observed in the capsaicin test, CcEO (100 mg/kg) was effective in the glutamate test. Naloxone, an opioid antagonist, did not affect the antinociceptive activity of CcEO in writhing test. In conclusion, the antinociceptive effect of CcEO could be explained, at least in part, by inhibition of the glutamatergic system.

No MeSH data available.


Related in: MedlinePlus