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Inhibitory Effects of Chemical Compounds Isolated from the Rhizome of Smilax glabra on Nitric Oxide and Tumor Necrosis Factor- α Production in Lipopolysaccharide-Induced RAW264.7 Cell.

Lu CL, Zhu W, Wang DM, Chen WL, Hu MM, Wang M, Xu XJ, Lu CJ - Evid Based Complement Alternat Med (2015)

Bottom Line: As a result, nine known compounds were isolated from the rhizome of S. glabra with the bioassay-guiding, and were identified as syringaresinol (1), lasiodiplodin (2), de-O-methyllasiodiplodin (3), syringic acid (4), 1,4-bis(4-hydroxy-3,5-dimethoxyphenyl)-2,3-bis(hydroxymethyl)-1,4-butanediol (5), lyoniresinol (6), trans-resveratrol (7), trans-caffeic acid methyl ester (8), and dihydrokaempferol (9).Results indicated that 4 and 7 showed significant inhibitory effects on NO production of RAW264.7 cells, and 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively.These findings strongly suggest that compounds 1, 2, 3, 4, 5, 7, and 9 were the potential anti-inflammatory active compositions of S. glabra.

View Article: PubMed Central - PubMed

Affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China ; Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China ; Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.

ABSTRACT
The rhizome of Smilax glabra has been used for a long time as both food and folk medicine in many countries. The present study focused on the active constituents from the rhizome of S. glabra, which possess potential anti-inflammatory activities. As a result, nine known compounds were isolated from the rhizome of S. glabra with the bioassay-guiding, and were identified as syringaresinol (1), lasiodiplodin (2), de-O-methyllasiodiplodin (3), syringic acid (4), 1,4-bis(4-hydroxy-3,5-dimethoxyphenyl)-2,3-bis(hydroxymethyl)-1,4-butanediol (5), lyoniresinol (6), trans-resveratrol (7), trans-caffeic acid methyl ester (8), and dihydrokaempferol (9). Among these compounds, 2 and 3 were isolated for the first time from S. glabra. In addition, the potential anti-inflammatory activities of the isolated compounds were evaluated in vitro in lipopolysaccharide- (LPS-) induced RAW264.7 cells. Results indicated that 4 and 7 showed significant inhibitory effects on NO production of RAW264.7 cells, and 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively. These findings strongly suggest that compounds 1, 2, 3, 4, 5, 7, and 9 were the potential anti-inflammatory active compositions of S. glabra.

No MeSH data available.


Related in: MedlinePlus

The inhibitory effects of the isolated compounds on NO (a) and TNF-α (b) productions in LPS-induced RAW264.7 cells. Cells were seeded into 24-well plates and incubated for 12 h and then treated with or without different concentrations of compounds for 24 h (for NO) or 14 h (for TNF-α). The culture supernatant was analyzed for nitrite and TNF-α production. Results shown are representative of three separate experiments. All conditions were run in triplicate, and data shows mean ± SD values.
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fig4: The inhibitory effects of the isolated compounds on NO (a) and TNF-α (b) productions in LPS-induced RAW264.7 cells. Cells were seeded into 24-well plates and incubated for 12 h and then treated with or without different concentrations of compounds for 24 h (for NO) or 14 h (for TNF-α). The culture supernatant was analyzed for nitrite and TNF-α production. Results shown are representative of three separate experiments. All conditions were run in triplicate, and data shows mean ± SD values.

Mentions: As shown in Figure 4(a), all isolated compounds, expect Comp. 8, exhibited dose-dependent inhibitory effects on LPS-induced NO production in RAW264.7 cells. Particularly, Comp. 7 at 4.4 and 0.4 μM, respectively, inhibited about 48% and 32% of LPS-induced NO production, while Comp. 4 at 5.1 and 0.5 μM inhibited about 25% and 19% of induced NO production, respectively. Compounds 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. But, Compounds 6 and 9 showed the lowest inhibitory effects, at level of IC10, respectively, 238.1 and 139.4 μM; just 32.7% and 21.3% of NO production were inhibited separately. The inhibitory effects of isolated compounds on TNF-α production presented in Figure 4(b) showed that all isolated compounds, expect Compounds 4 and 9, exhibited dose-dependent inhibitory effects on LPS-induced TNF-α production in RAW264.7 cells. Compounds 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively.


Inhibitory Effects of Chemical Compounds Isolated from the Rhizome of Smilax glabra on Nitric Oxide and Tumor Necrosis Factor- α Production in Lipopolysaccharide-Induced RAW264.7 Cell.

Lu CL, Zhu W, Wang DM, Chen WL, Hu MM, Wang M, Xu XJ, Lu CJ - Evid Based Complement Alternat Med (2015)

The inhibitory effects of the isolated compounds on NO (a) and TNF-α (b) productions in LPS-induced RAW264.7 cells. Cells were seeded into 24-well plates and incubated for 12 h and then treated with or without different concentrations of compounds for 24 h (for NO) or 14 h (for TNF-α). The culture supernatant was analyzed for nitrite and TNF-α production. Results shown are representative of three separate experiments. All conditions were run in triplicate, and data shows mean ± SD values.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4363603&req=5

fig4: The inhibitory effects of the isolated compounds on NO (a) and TNF-α (b) productions in LPS-induced RAW264.7 cells. Cells were seeded into 24-well plates and incubated for 12 h and then treated with or without different concentrations of compounds for 24 h (for NO) or 14 h (for TNF-α). The culture supernatant was analyzed for nitrite and TNF-α production. Results shown are representative of three separate experiments. All conditions were run in triplicate, and data shows mean ± SD values.
Mentions: As shown in Figure 4(a), all isolated compounds, expect Comp. 8, exhibited dose-dependent inhibitory effects on LPS-induced NO production in RAW264.7 cells. Particularly, Comp. 7 at 4.4 and 0.4 μM, respectively, inhibited about 48% and 32% of LPS-induced NO production, while Comp. 4 at 5.1 and 0.5 μM inhibited about 25% and 19% of induced NO production, respectively. Compounds 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. But, Compounds 6 and 9 showed the lowest inhibitory effects, at level of IC10, respectively, 238.1 and 139.4 μM; just 32.7% and 21.3% of NO production were inhibited separately. The inhibitory effects of isolated compounds on TNF-α production presented in Figure 4(b) showed that all isolated compounds, expect Compounds 4 and 9, exhibited dose-dependent inhibitory effects on LPS-induced TNF-α production in RAW264.7 cells. Compounds 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively.

Bottom Line: As a result, nine known compounds were isolated from the rhizome of S. glabra with the bioassay-guiding, and were identified as syringaresinol (1), lasiodiplodin (2), de-O-methyllasiodiplodin (3), syringic acid (4), 1,4-bis(4-hydroxy-3,5-dimethoxyphenyl)-2,3-bis(hydroxymethyl)-1,4-butanediol (5), lyoniresinol (6), trans-resveratrol (7), trans-caffeic acid methyl ester (8), and dihydrokaempferol (9).Results indicated that 4 and 7 showed significant inhibitory effects on NO production of RAW264.7 cells, and 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively.These findings strongly suggest that compounds 1, 2, 3, 4, 5, 7, and 9 were the potential anti-inflammatory active compositions of S. glabra.

View Article: PubMed Central - PubMed

Affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China ; Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China ; Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.

ABSTRACT
The rhizome of Smilax glabra has been used for a long time as both food and folk medicine in many countries. The present study focused on the active constituents from the rhizome of S. glabra, which possess potential anti-inflammatory activities. As a result, nine known compounds were isolated from the rhizome of S. glabra with the bioassay-guiding, and were identified as syringaresinol (1), lasiodiplodin (2), de-O-methyllasiodiplodin (3), syringic acid (4), 1,4-bis(4-hydroxy-3,5-dimethoxyphenyl)-2,3-bis(hydroxymethyl)-1,4-butanediol (5), lyoniresinol (6), trans-resveratrol (7), trans-caffeic acid methyl ester (8), and dihydrokaempferol (9). Among these compounds, 2 and 3 were isolated for the first time from S. glabra. In addition, the potential anti-inflammatory activities of the isolated compounds were evaluated in vitro in lipopolysaccharide- (LPS-) induced RAW264.7 cells. Results indicated that 4 and 7 showed significant inhibitory effects on NO production of RAW264.7 cells, and 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively. These findings strongly suggest that compounds 1, 2, 3, 4, 5, 7, and 9 were the potential anti-inflammatory active compositions of S. glabra.

No MeSH data available.


Related in: MedlinePlus