Limits...
Royal Jelly and its dual role in TNBS colitis in mice.

Manzo LP, de-Faria FM, Dunder RJ, Rabelo-Socca EA, Consonni SR, de Almeida AC, Souza-Brito AR, Luiz-Ferreira A - ScientificWorldJournal (2015)

Bottom Line: Royal Jelly (RJ) is widely consumed in diets throughout the world due to its beneficial effects: antioxidant, antitumor and anti-inflammatory.In conclusion, RJ showed anti-inflammatory and antioxidant properties in experimental colitis, resulting in the amelioration of the macroscopic and histological analyses.These results corroborate with the RJ supplementation in diets.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medical Sciences, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil ; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil.

ABSTRACT
Royal Jelly (RJ) is widely consumed in diets throughout the world due to its beneficial effects: antioxidant, antitumor and anti-inflammatory. We have investigated the role of RJ in the development of TNBS colitis in mice. Colitis was induced by a rectal instillation of TNBS at 0.1 mL per mouse. Intestine samples of the animals orally treated with RJ (100, 150, and 200 mg/kg) were collected for antioxidant assays (GSH and GSH-Px), proinflammatory protein quantification (COX-2 and NF-κB), and histological analyses. RJ 100 mg/kg maintained GSH levels and increased the activity of GSH-Px, downregulated key inflammatory mediators (COX-2 and NF-κB), and decreased the lesions caused by TNBS as shown by the histological analyses. In conclusion, RJ showed anti-inflammatory and antioxidant properties in experimental colitis, resulting in the amelioration of the macroscopic and histological analyses. These results corroborate with the RJ supplementation in diets.

No MeSH data available.


Related in: MedlinePlus

Representative Western blot analysis of COX-2 proteins. Densitometric data were studied following normalization to the control (Ponceau). The results are representative of three experiments performed on different samples and data are expressed as mean ± SEM. ANOVA followed by Dunnett's t-test. aP < 0.05, bP < 0.01, and cP < 0.001 are significantly different from TNBS.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4363584&req=5

fig4: Representative Western blot analysis of COX-2 proteins. Densitometric data were studied following normalization to the control (Ponceau). The results are representative of three experiments performed on different samples and data are expressed as mean ± SEM. ANOVA followed by Dunnett's t-test. aP < 0.05, bP < 0.01, and cP < 0.001 are significantly different from TNBS.

Mentions: NF-κB and COX-2 from colonic mucosa were measured by western blotting (Figures 3 and 4). The levels of expression of NF-κB p65 were detected in low quantity in nuclei of normal mucosa whereas a high expression of nuclear factor appeared in colon mucosa from control TNBS colitic group. Nonetheless, upon pretreatment with RJ 100 mg/kg, the expression of NF-κB p65 was kept at lower levels than TNBS group (Figure 3). As shown in Figure 4, exposure of colon to TNBS caused strong expression of COX-2; on the other hand, RJ at 100 mg/kg induced downregulation of COX-2 when compared with TNBS group (P < 0.001).


Royal Jelly and its dual role in TNBS colitis in mice.

Manzo LP, de-Faria FM, Dunder RJ, Rabelo-Socca EA, Consonni SR, de Almeida AC, Souza-Brito AR, Luiz-Ferreira A - ScientificWorldJournal (2015)

Representative Western blot analysis of COX-2 proteins. Densitometric data were studied following normalization to the control (Ponceau). The results are representative of three experiments performed on different samples and data are expressed as mean ± SEM. ANOVA followed by Dunnett's t-test. aP < 0.05, bP < 0.01, and cP < 0.001 are significantly different from TNBS.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4363584&req=5

fig4: Representative Western blot analysis of COX-2 proteins. Densitometric data were studied following normalization to the control (Ponceau). The results are representative of three experiments performed on different samples and data are expressed as mean ± SEM. ANOVA followed by Dunnett's t-test. aP < 0.05, bP < 0.01, and cP < 0.001 are significantly different from TNBS.
Mentions: NF-κB and COX-2 from colonic mucosa were measured by western blotting (Figures 3 and 4). The levels of expression of NF-κB p65 were detected in low quantity in nuclei of normal mucosa whereas a high expression of nuclear factor appeared in colon mucosa from control TNBS colitic group. Nonetheless, upon pretreatment with RJ 100 mg/kg, the expression of NF-κB p65 was kept at lower levels than TNBS group (Figure 3). As shown in Figure 4, exposure of colon to TNBS caused strong expression of COX-2; on the other hand, RJ at 100 mg/kg induced downregulation of COX-2 when compared with TNBS group (P < 0.001).

Bottom Line: Royal Jelly (RJ) is widely consumed in diets throughout the world due to its beneficial effects: antioxidant, antitumor and anti-inflammatory.In conclusion, RJ showed anti-inflammatory and antioxidant properties in experimental colitis, resulting in the amelioration of the macroscopic and histological analyses.These results corroborate with the RJ supplementation in diets.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medical Sciences, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil ; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil.

ABSTRACT
Royal Jelly (RJ) is widely consumed in diets throughout the world due to its beneficial effects: antioxidant, antitumor and anti-inflammatory. We have investigated the role of RJ in the development of TNBS colitis in mice. Colitis was induced by a rectal instillation of TNBS at 0.1 mL per mouse. Intestine samples of the animals orally treated with RJ (100, 150, and 200 mg/kg) were collected for antioxidant assays (GSH and GSH-Px), proinflammatory protein quantification (COX-2 and NF-κB), and histological analyses. RJ 100 mg/kg maintained GSH levels and increased the activity of GSH-Px, downregulated key inflammatory mediators (COX-2 and NF-κB), and decreased the lesions caused by TNBS as shown by the histological analyses. In conclusion, RJ showed anti-inflammatory and antioxidant properties in experimental colitis, resulting in the amelioration of the macroscopic and histological analyses. These results corroborate with the RJ supplementation in diets.

No MeSH data available.


Related in: MedlinePlus