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Etoposide incorporated into camel milk phospholipids liposomes shows increased activity against fibrosarcoma in a mouse model.

Maswadeh HM, Aljarbou AN, Alorainy MS, Alsharidah MS, Khan MA - Biomed Res Int (2015)

Bottom Line: Anticancer drug etoposide (ETP) was entrapped in liposomes, prepared from camel milk phospholipids, to determine its activity against fibrosarcoma in a murine model.The tumor-bearing mice treated with ETP-Cam-liposomes showed slow progression of tumors and increased survival compared to free ETP or ETP-DPPC-liposomes.These results suggest that ETP-Cam-liposomes may prove to be a better drug delivery system for anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraidah 51412, Saudi Arabia.

ABSTRACT
Phospholipids were isolated from camel milk and identified by using high performance liquid chromatography and gas chromatography-mass spectrometry (GC/MS). Anticancer drug etoposide (ETP) was entrapped in liposomes, prepared from camel milk phospholipids, to determine its activity against fibrosarcoma in a murine model. Fibrosarcoma was induced in mice by injecting benzopyrene (BAP) and tumor-bearing mice were treated with various formulations of etoposide, including etoposide entrapped camel milk phospholipids liposomes (ETP-Cam-liposomes) and etoposide-loaded DPPC-liposomes (ETP-DPPC-liposomes). The tumor-bearing mice treated with ETP-Cam-liposomes showed slow progression of tumors and increased survival compared to free ETP or ETP-DPPC-liposomes. These results suggest that ETP-Cam-liposomes may prove to be a better drug delivery system for anticancer drugs.

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Related in: MedlinePlus

Size distribution for liposomes prepared from DPPC. (a) MLVs liposomes before extrusion, (b) liposomes after extrusion 10 times through 200 nm polycarbonate membrane, and (c) SUVs liposomes after extrusion 10 times through 100 nm polycarbonate membrane.
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fig4: Size distribution for liposomes prepared from DPPC. (a) MLVs liposomes before extrusion, (b) liposomes after extrusion 10 times through 200 nm polycarbonate membrane, and (c) SUVs liposomes after extrusion 10 times through 100 nm polycarbonate membrane.

Mentions: Microphotographs obtained from the optical microscopy show that MLVs liposomes were successfully prepared from a mixture of phospholipid isolated from camel milk by using the thin film hydration method (Figure 3). The vesicle size distributions for DPPC-liposomes and Cam-liposomes before and after the extrusion through 200 nm followed by 100 nm polycarbonate membrane (10 times for each) were determined using a Malvern Mastersizer. Figures 4 and 5 show that the repetitive extrusion of MLVs through the stacked polycarbonate membrane with 200 nm followed by 100 nm pore size after 10 freeze-thaw cycles resulted in small unilamellar vesicles (SUVs) exhibiting a relatively homogeneous size distribution. The size distribution for DPPC-liposomes before extrusion was 2312 nm for the first peak with 80.65% intensity and 5557 nm for the second peak with 19.4% intensity (Figure 4(a)). The size distribution after 10-time extrusions through 200 nm polycarbonate membrane was 181.3 nm with 91.5% intensity for the first peak and 5000 nm with 8.5% intensity for the second peak (Figure 4(b)). While after 10-time extrusions through 100 nm polycarbonate membrane, only one peak was obtained at 113 nm with 100% intensity (Figure 4(c)).


Etoposide incorporated into camel milk phospholipids liposomes shows increased activity against fibrosarcoma in a mouse model.

Maswadeh HM, Aljarbou AN, Alorainy MS, Alsharidah MS, Khan MA - Biomed Res Int (2015)

Size distribution for liposomes prepared from DPPC. (a) MLVs liposomes before extrusion, (b) liposomes after extrusion 10 times through 200 nm polycarbonate membrane, and (c) SUVs liposomes after extrusion 10 times through 100 nm polycarbonate membrane.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4363510&req=5

fig4: Size distribution for liposomes prepared from DPPC. (a) MLVs liposomes before extrusion, (b) liposomes after extrusion 10 times through 200 nm polycarbonate membrane, and (c) SUVs liposomes after extrusion 10 times through 100 nm polycarbonate membrane.
Mentions: Microphotographs obtained from the optical microscopy show that MLVs liposomes were successfully prepared from a mixture of phospholipid isolated from camel milk by using the thin film hydration method (Figure 3). The vesicle size distributions for DPPC-liposomes and Cam-liposomes before and after the extrusion through 200 nm followed by 100 nm polycarbonate membrane (10 times for each) were determined using a Malvern Mastersizer. Figures 4 and 5 show that the repetitive extrusion of MLVs through the stacked polycarbonate membrane with 200 nm followed by 100 nm pore size after 10 freeze-thaw cycles resulted in small unilamellar vesicles (SUVs) exhibiting a relatively homogeneous size distribution. The size distribution for DPPC-liposomes before extrusion was 2312 nm for the first peak with 80.65% intensity and 5557 nm for the second peak with 19.4% intensity (Figure 4(a)). The size distribution after 10-time extrusions through 200 nm polycarbonate membrane was 181.3 nm with 91.5% intensity for the first peak and 5000 nm with 8.5% intensity for the second peak (Figure 4(b)). While after 10-time extrusions through 100 nm polycarbonate membrane, only one peak was obtained at 113 nm with 100% intensity (Figure 4(c)).

Bottom Line: Anticancer drug etoposide (ETP) was entrapped in liposomes, prepared from camel milk phospholipids, to determine its activity against fibrosarcoma in a murine model.The tumor-bearing mice treated with ETP-Cam-liposomes showed slow progression of tumors and increased survival compared to free ETP or ETP-DPPC-liposomes.These results suggest that ETP-Cam-liposomes may prove to be a better drug delivery system for anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraidah 51412, Saudi Arabia.

ABSTRACT
Phospholipids were isolated from camel milk and identified by using high performance liquid chromatography and gas chromatography-mass spectrometry (GC/MS). Anticancer drug etoposide (ETP) was entrapped in liposomes, prepared from camel milk phospholipids, to determine its activity against fibrosarcoma in a murine model. Fibrosarcoma was induced in mice by injecting benzopyrene (BAP) and tumor-bearing mice were treated with various formulations of etoposide, including etoposide entrapped camel milk phospholipids liposomes (ETP-Cam-liposomes) and etoposide-loaded DPPC-liposomes (ETP-DPPC-liposomes). The tumor-bearing mice treated with ETP-Cam-liposomes showed slow progression of tumors and increased survival compared to free ETP or ETP-DPPC-liposomes. These results suggest that ETP-Cam-liposomes may prove to be a better drug delivery system for anticancer drugs.

Show MeSH
Related in: MedlinePlus