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Long-term Treatment with Oriental Medicinal Herb Artemisia princeps Alters Neuroplasticity in a Rat Model of Ovarian Hormone Deficiency.

Kim HB, Kwon BJ, Cho HJ, Kim JW, Chon JW, Do MH, Park SY, Kim SY, Maeng SH, Park YK, Park JH - Exp Neurobiol (2015)

Bottom Line: Artemisia princeps (AP) is a flowering perennial used as a traditional medicine and dietary supplement across East Asia.No study has yet assessed its effects on synaptic plasticity in hippocampus and much less in a model of ovarian hormone deficiency.Ovariectomized rats demonstrated lower trabecular mean bone mineral densities than sham, validating the establishment of pathology.

View Article: PubMed Central - PubMed

Affiliation: Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Korea.

ABSTRACT
Artemisia princeps (AP) is a flowering perennial used as a traditional medicine and dietary supplement across East Asia. No study has yet assessed its effects on synaptic plasticity in hippocampus and much less in a model of ovarian hormone deficiency. We examined the influence of chronic oral AP ethanol extract treatment in ovariectomized rats on the induction of long-term depression in a representative synapse (CA3-CA1) of the hippocampus. Ovariectomized rats demonstrated lower trabecular mean bone mineral densities than sham, validating the establishment of pathology. Against this background of pathology, AP-treated ovariectomized rats exhibited attenuated long-term depression (LTD) in CA1 relative to water-treated controls as measured by increased field excitatory post-synaptic potentials (fEPSP) activation averages over the post-stimulation period. While pathological significance of long-term depression (LTD) in ovariectomized rats is conflicting, that AP treatment significantly affected its induction offers justification for further study of its influences on plasticity and its related disorders.

No MeSH data available.


Related in: MedlinePlus

Integrated activation-period microelectrode array field potential sums following low-frequency stimulation of hippocampal slice cultures. Sum of all integrated activation-period field potentials from three of 59 single microelectrode recordings of hippocampal slice cultures from 33-week-old ovariectomized rats treated with distilled water (OVX, n=3 slices), 10 mg/kg/d sodium alendronate (ALEN, n=3 slices), or 300 mg/kg/d Artemisia princeps ethanol extract (AP, n=3 slices) daily by oral gavage (0.1 mL/kg) for 15 weeks following a 13-week pretreatment period. Recordings followed 15 minutes of low-frequency stimulation (100 mA, 1Hz) to the CA2 stratum radiatum to stimulate LTD-inducing Schaffer collateral signaling. (A) Time course of activity totals. Starting at 10 minutes following stimulation, OVX rats exhibited significantly lower activation than that of ALEN or AP rats, a difference maintained until the end of the experiment. (B) Group averages of normalized total activity for 50 minutes following stimulation. ALEN and AP activation were both significantly higher than that of the OVX group (F2,7=26.797; p=0.001; Scheffe's post hoc S mean difference 11.88%, p<0.01; mean difference 10.00%, p<0.05, respectively) but did not differ significantly from each other. Values represent means±SEM. Stars represent significant between-group differences as calculated by Scheffe's S post hoc analysis with alpha set at p<0.05.
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Figure 4: Integrated activation-period microelectrode array field potential sums following low-frequency stimulation of hippocampal slice cultures. Sum of all integrated activation-period field potentials from three of 59 single microelectrode recordings of hippocampal slice cultures from 33-week-old ovariectomized rats treated with distilled water (OVX, n=3 slices), 10 mg/kg/d sodium alendronate (ALEN, n=3 slices), or 300 mg/kg/d Artemisia princeps ethanol extract (AP, n=3 slices) daily by oral gavage (0.1 mL/kg) for 15 weeks following a 13-week pretreatment period. Recordings followed 15 minutes of low-frequency stimulation (100 mA, 1Hz) to the CA2 stratum radiatum to stimulate LTD-inducing Schaffer collateral signaling. (A) Time course of activity totals. Starting at 10 minutes following stimulation, OVX rats exhibited significantly lower activation than that of ALEN or AP rats, a difference maintained until the end of the experiment. (B) Group averages of normalized total activity for 50 minutes following stimulation. ALEN and AP activation were both significantly higher than that of the OVX group (F2,7=26.797; p=0.001; Scheffe's post hoc S mean difference 11.88%, p<0.01; mean difference 10.00%, p<0.05, respectively) but did not differ significantly from each other. Values represent means±SEM. Stars represent significant between-group differences as calculated by Scheffe's S post hoc analysis with alpha set at p<0.05.

Mentions: Total activation values from each of the 75 minutes of fEPSP recording from CA1 were averaged across CA1 channels (n=2-4 per slice) and normalized to percent of baseline, which was defined as the average of the first ten minutes of pre-stimulation recording. A repeated measures ANOVA with fixed factor treatment and within-subjects repeated measure time (before, during, and following stimulation) on individual subject averages showed a significant within-subject effect of time (F2,7=90.521, p<0.001), a significant between-subject effect of treatment (F2,7=24.929, p<0.01), and a significant within-subject interaction between time and treatment (F2,7=13.632, p<0.001) on total activation averages. Post-hoc analysis for treatment with Scheffe's S procedure revealed significantly higher values for both ALEN and AP treatment groups relative to water-treated ovariectomized controls (mean difference 10.07%, p<0.01; 8.78%, p<0.01, respectively) but no significant difference of AP from ALEN (mean difference -1.29%, p=0.704). Univariate ANOVA showed no effect of treatment on time-averaged fEPSP total activation percent before stimulation as they were, by definition, normalized to a mean of 100% for each group, but showed a significant effect both during (F2,7=11.241; p<0.01) and after (F2,7=26.797; p=0.001). Scheffe's S post-hoc analysis revealed significantly higher values for ALEN and AP treatment groups relative to water-treated ovariectomized controls during stimulation (94.98±2.19%, mean difference 11.88%, p<0.01; 93.10±0.31%, mean difference 10.00%, p<0.05, respectively; control 83.10±2.00%) and after stimulation (92.00±2.35%, mean difference 18.00%, p<0.01; 90.00±0.58%, mean difference 16.68%, p<0.01, respectively; control 73.67±1.20%) but no significant difference of AP relative to ALEN during either time frame (mean difference during stimulation -1.87%, p=0.780; mean difference after stimulation -2.00%, p=0.761). See Fig. 4 for activation trends across time for each group.


Long-term Treatment with Oriental Medicinal Herb Artemisia princeps Alters Neuroplasticity in a Rat Model of Ovarian Hormone Deficiency.

Kim HB, Kwon BJ, Cho HJ, Kim JW, Chon JW, Do MH, Park SY, Kim SY, Maeng SH, Park YK, Park JH - Exp Neurobiol (2015)

Integrated activation-period microelectrode array field potential sums following low-frequency stimulation of hippocampal slice cultures. Sum of all integrated activation-period field potentials from three of 59 single microelectrode recordings of hippocampal slice cultures from 33-week-old ovariectomized rats treated with distilled water (OVX, n=3 slices), 10 mg/kg/d sodium alendronate (ALEN, n=3 slices), or 300 mg/kg/d Artemisia princeps ethanol extract (AP, n=3 slices) daily by oral gavage (0.1 mL/kg) for 15 weeks following a 13-week pretreatment period. Recordings followed 15 minutes of low-frequency stimulation (100 mA, 1Hz) to the CA2 stratum radiatum to stimulate LTD-inducing Schaffer collateral signaling. (A) Time course of activity totals. Starting at 10 minutes following stimulation, OVX rats exhibited significantly lower activation than that of ALEN or AP rats, a difference maintained until the end of the experiment. (B) Group averages of normalized total activity for 50 minutes following stimulation. ALEN and AP activation were both significantly higher than that of the OVX group (F2,7=26.797; p=0.001; Scheffe's post hoc S mean difference 11.88%, p<0.01; mean difference 10.00%, p<0.05, respectively) but did not differ significantly from each other. Values represent means±SEM. Stars represent significant between-group differences as calculated by Scheffe's S post hoc analysis with alpha set at p<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4363335&req=5

Figure 4: Integrated activation-period microelectrode array field potential sums following low-frequency stimulation of hippocampal slice cultures. Sum of all integrated activation-period field potentials from three of 59 single microelectrode recordings of hippocampal slice cultures from 33-week-old ovariectomized rats treated with distilled water (OVX, n=3 slices), 10 mg/kg/d sodium alendronate (ALEN, n=3 slices), or 300 mg/kg/d Artemisia princeps ethanol extract (AP, n=3 slices) daily by oral gavage (0.1 mL/kg) for 15 weeks following a 13-week pretreatment period. Recordings followed 15 minutes of low-frequency stimulation (100 mA, 1Hz) to the CA2 stratum radiatum to stimulate LTD-inducing Schaffer collateral signaling. (A) Time course of activity totals. Starting at 10 minutes following stimulation, OVX rats exhibited significantly lower activation than that of ALEN or AP rats, a difference maintained until the end of the experiment. (B) Group averages of normalized total activity for 50 minutes following stimulation. ALEN and AP activation were both significantly higher than that of the OVX group (F2,7=26.797; p=0.001; Scheffe's post hoc S mean difference 11.88%, p<0.01; mean difference 10.00%, p<0.05, respectively) but did not differ significantly from each other. Values represent means±SEM. Stars represent significant between-group differences as calculated by Scheffe's S post hoc analysis with alpha set at p<0.05.
Mentions: Total activation values from each of the 75 minutes of fEPSP recording from CA1 were averaged across CA1 channels (n=2-4 per slice) and normalized to percent of baseline, which was defined as the average of the first ten minutes of pre-stimulation recording. A repeated measures ANOVA with fixed factor treatment and within-subjects repeated measure time (before, during, and following stimulation) on individual subject averages showed a significant within-subject effect of time (F2,7=90.521, p<0.001), a significant between-subject effect of treatment (F2,7=24.929, p<0.01), and a significant within-subject interaction between time and treatment (F2,7=13.632, p<0.001) on total activation averages. Post-hoc analysis for treatment with Scheffe's S procedure revealed significantly higher values for both ALEN and AP treatment groups relative to water-treated ovariectomized controls (mean difference 10.07%, p<0.01; 8.78%, p<0.01, respectively) but no significant difference of AP from ALEN (mean difference -1.29%, p=0.704). Univariate ANOVA showed no effect of treatment on time-averaged fEPSP total activation percent before stimulation as they were, by definition, normalized to a mean of 100% for each group, but showed a significant effect both during (F2,7=11.241; p<0.01) and after (F2,7=26.797; p=0.001). Scheffe's S post-hoc analysis revealed significantly higher values for ALEN and AP treatment groups relative to water-treated ovariectomized controls during stimulation (94.98±2.19%, mean difference 11.88%, p<0.01; 93.10±0.31%, mean difference 10.00%, p<0.05, respectively; control 83.10±2.00%) and after stimulation (92.00±2.35%, mean difference 18.00%, p<0.01; 90.00±0.58%, mean difference 16.68%, p<0.01, respectively; control 73.67±1.20%) but no significant difference of AP relative to ALEN during either time frame (mean difference during stimulation -1.87%, p=0.780; mean difference after stimulation -2.00%, p=0.761). See Fig. 4 for activation trends across time for each group.

Bottom Line: Artemisia princeps (AP) is a flowering perennial used as a traditional medicine and dietary supplement across East Asia.No study has yet assessed its effects on synaptic plasticity in hippocampus and much less in a model of ovarian hormone deficiency.Ovariectomized rats demonstrated lower trabecular mean bone mineral densities than sham, validating the establishment of pathology.

View Article: PubMed Central - PubMed

Affiliation: Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Korea.

ABSTRACT
Artemisia princeps (AP) is a flowering perennial used as a traditional medicine and dietary supplement across East Asia. No study has yet assessed its effects on synaptic plasticity in hippocampus and much less in a model of ovarian hormone deficiency. We examined the influence of chronic oral AP ethanol extract treatment in ovariectomized rats on the induction of long-term depression in a representative synapse (CA3-CA1) of the hippocampus. Ovariectomized rats demonstrated lower trabecular mean bone mineral densities than sham, validating the establishment of pathology. Against this background of pathology, AP-treated ovariectomized rats exhibited attenuated long-term depression (LTD) in CA1 relative to water-treated controls as measured by increased field excitatory post-synaptic potentials (fEPSP) activation averages over the post-stimulation period. While pathological significance of long-term depression (LTD) in ovariectomized rats is conflicting, that AP treatment significantly affected its induction offers justification for further study of its influences on plasticity and its related disorders.

No MeSH data available.


Related in: MedlinePlus