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Effect of dual treatment with SDF-1 and BMP-2 on ectopic and orthotopic bone formation.

Lee CH, Jin MU, Jung HM, Lee JT, Kwon TG - PLoS ONE (2015)

Bottom Line: The purpose of this study was to evaluate the potential effects of simultaneous SDF-1 and BMP-2 treatment on bone formation.SDF-1-only-treated implants did not yield significant in vivo bone formation and SDF-1 treatment did not enhance BMP-2-induced ectopic and orthotopic bone regeneration.In vitro experiments showed that concomitant use of BMP-2 and SDF-1 had no additive effect on osteoblastic differentiation, cell migration or angiogenesis compared to BMP-2 or SDF-1 treatment alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea.

ABSTRACT

Purposes: The potent stem cell homing factor stromal cell-derived factor-1 (SDF-1) actively recruits mesenchymal stem cells from circulation and from local bone marrow. It is well established that bone morphogenetic protein-2 (BMP-2) induces ectopic and orthotopic bone formation. However, the exact synergistic effects of BMP-2 and SDF-1 in ectopic and orthotopic bone regeneration models have not been fully investigated. The purpose of this study was to evaluate the potential effects of simultaneous SDF-1 and BMP-2 treatment on bone formation.

Materials and methods: Various doses of SDF-1 were loaded onto collagen sponges with or without BMP-2.These sponges were implanted into subcutaneous pockets and critical-size calvarial defects in C57BL/6 mice. The specimens were harvested 4 weeks post-surgery and the degree of bone formation in specimens was evaluated by histomorphometric and radiographic density analyses. Osteogenic potential and migration capacity of mesenchymal cells and capillary tube formation of endothelial cells following dual treatment with SDF-1 and BMP-2 were evaluated with in vitro assays.

Results: SDF-1-only-treated implants did not yield significant in vivo bone formation and SDF-1 treatment did not enhance BMP-2-induced ectopic and orthotopic bone regeneration. In vitro experiments showed that concomitant use of BMP-2 and SDF-1 had no additive effect on osteoblastic differentiation, cell migration or angiogenesis compared to BMP-2 or SDF-1 treatment alone.

Conclusions: These findings imply that sequence-controlled application of SDF-1 and BMP-2 must be further investigated for the enhancement of robust osteogenesis in bone defects.

No MeSH data available.


Related in: MedlinePlus

The effect of simultaneous treatment with SDF-1 and BMP-2 on orthotopic bone formation.Soft X-ray examination was carried out after implantation of collagen sponges to critical-size calvarial defects with/without BMP-2 (2.5μg) and SDF-1 (0, 0.1, 0.5, or 1 μg/collagen sponge) in mice. (A) Representative radiographic images, (B) Quantification of bone regeneration by radiographic density at 4 weeks post-implantation (*, p < 0.05).
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pone.0120051.g003: The effect of simultaneous treatment with SDF-1 and BMP-2 on orthotopic bone formation.Soft X-ray examination was carried out after implantation of collagen sponges to critical-size calvarial defects with/without BMP-2 (2.5μg) and SDF-1 (0, 0.1, 0.5, or 1 μg/collagen sponge) in mice. (A) Representative radiographic images, (B) Quantification of bone regeneration by radiographic density at 4 weeks post-implantation (*, p < 0.05).

Mentions: Similar to the ectopic bone formation model, all BMP-2-treated samples exhibited significantly more orthotopic bone formation compared with all samples lacking BMP-2 treatment in radiographic assessment. Treatment with 1μg SDF-1 resulted in slightly increased (31.8%) cranial bone formation at the critical-size calvarial defect compared to 0.1μg SDF-1treatment (p < 0.05); however, SDF-1 did not increase BMP-2-induced bone formation at any of the doses tested (Fig. 3).


Effect of dual treatment with SDF-1 and BMP-2 on ectopic and orthotopic bone formation.

Lee CH, Jin MU, Jung HM, Lee JT, Kwon TG - PLoS ONE (2015)

The effect of simultaneous treatment with SDF-1 and BMP-2 on orthotopic bone formation.Soft X-ray examination was carried out after implantation of collagen sponges to critical-size calvarial defects with/without BMP-2 (2.5μg) and SDF-1 (0, 0.1, 0.5, or 1 μg/collagen sponge) in mice. (A) Representative radiographic images, (B) Quantification of bone regeneration by radiographic density at 4 weeks post-implantation (*, p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363323&req=5

pone.0120051.g003: The effect of simultaneous treatment with SDF-1 and BMP-2 on orthotopic bone formation.Soft X-ray examination was carried out after implantation of collagen sponges to critical-size calvarial defects with/without BMP-2 (2.5μg) and SDF-1 (0, 0.1, 0.5, or 1 μg/collagen sponge) in mice. (A) Representative radiographic images, (B) Quantification of bone regeneration by radiographic density at 4 weeks post-implantation (*, p < 0.05).
Mentions: Similar to the ectopic bone formation model, all BMP-2-treated samples exhibited significantly more orthotopic bone formation compared with all samples lacking BMP-2 treatment in radiographic assessment. Treatment with 1μg SDF-1 resulted in slightly increased (31.8%) cranial bone formation at the critical-size calvarial defect compared to 0.1μg SDF-1treatment (p < 0.05); however, SDF-1 did not increase BMP-2-induced bone formation at any of the doses tested (Fig. 3).

Bottom Line: The purpose of this study was to evaluate the potential effects of simultaneous SDF-1 and BMP-2 treatment on bone formation.SDF-1-only-treated implants did not yield significant in vivo bone formation and SDF-1 treatment did not enhance BMP-2-induced ectopic and orthotopic bone regeneration.In vitro experiments showed that concomitant use of BMP-2 and SDF-1 had no additive effect on osteoblastic differentiation, cell migration or angiogenesis compared to BMP-2 or SDF-1 treatment alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea.

ABSTRACT

Purposes: The potent stem cell homing factor stromal cell-derived factor-1 (SDF-1) actively recruits mesenchymal stem cells from circulation and from local bone marrow. It is well established that bone morphogenetic protein-2 (BMP-2) induces ectopic and orthotopic bone formation. However, the exact synergistic effects of BMP-2 and SDF-1 in ectopic and orthotopic bone regeneration models have not been fully investigated. The purpose of this study was to evaluate the potential effects of simultaneous SDF-1 and BMP-2 treatment on bone formation.

Materials and methods: Various doses of SDF-1 were loaded onto collagen sponges with or without BMP-2.These sponges were implanted into subcutaneous pockets and critical-size calvarial defects in C57BL/6 mice. The specimens were harvested 4 weeks post-surgery and the degree of bone formation in specimens was evaluated by histomorphometric and radiographic density analyses. Osteogenic potential and migration capacity of mesenchymal cells and capillary tube formation of endothelial cells following dual treatment with SDF-1 and BMP-2 were evaluated with in vitro assays.

Results: SDF-1-only-treated implants did not yield significant in vivo bone formation and SDF-1 treatment did not enhance BMP-2-induced ectopic and orthotopic bone regeneration. In vitro experiments showed that concomitant use of BMP-2 and SDF-1 had no additive effect on osteoblastic differentiation, cell migration or angiogenesis compared to BMP-2 or SDF-1 treatment alone.

Conclusions: These findings imply that sequence-controlled application of SDF-1 and BMP-2 must be further investigated for the enhancement of robust osteogenesis in bone defects.

No MeSH data available.


Related in: MedlinePlus