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Effects of resveratrol treatment on bone and cartilage in obese diabetic mice.

Cooley J, Broderick TL, Al-Nakkash L, Plochocki JH - J Diabetes Metab Disord (2015)

Bottom Line: Resveratrol treatment significantly increased cortical area in both ob/ob and control mice, but did not improve cross-sectional indicators of resistance to bending.Resveratrol treatment of ob/ob mice had mixed effects on bone histomorphometry at the femoral midshaft.Treatment increased cortical area but decreased bone length.

View Article: PubMed Central - PubMed

Affiliation: Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ USA.

ABSTRACT

Background: Resveratrol is a polyphenolic phytoalexin that has been shown to exhibit osteoprotective and chondroprotective properties. We examine the effects of resveratrol treatment on bone and cartilage tissue of obese, diabetic ob/ob mice.

Methods: Eight-week-old ob/ob and lean control mice were given trans-resveratrol at an oral dose of 25 mg/kg for 3 weeks. Histomorphometric and cross-sectional-geometric variables were analyzed.

Results: Ob/ob mice in our study exhibit significantly reduced femoral length, resistance to loading, and tibial growth plate total area and calcified area than lean controls (P < 0.05). Resveratrol treatment significantly increased cortical area in both ob/ob and control mice, but did not improve cross-sectional indicators of resistance to bending. Resveratrol treatment also reduced tibial length and calcified growth plate cartilage area in comparison to untreated mice (P < 0.05).

Conclusion: Resveratrol treatment of ob/ob mice had mixed effects on bone histomorphometry at the femoral midshaft. Treatment increased cortical area but decreased bone length.

No MeSH data available.


Related in: MedlinePlus

Photo micrographs of the proximal tibial growth plate of lean control (A), lean resveratrol-treated (B), obese control (C), and obese resveratrol-treated (D) mice. Obese mice had growth plate chondrocytes that appeared to be more disorganized than those of lean mice. Mice treated with resveratrol had significantly greater calcified cartilage areas than controls (P < 0.05). Toluidine blue and aqueous fast green, 20x magnification. Scale bar = 100 μm. * calcified cartilage; ** bone.
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Fig2: Photo micrographs of the proximal tibial growth plate of lean control (A), lean resveratrol-treated (B), obese control (C), and obese resveratrol-treated (D) mice. Obese mice had growth plate chondrocytes that appeared to be more disorganized than those of lean mice. Mice treated with resveratrol had significantly greater calcified cartilage areas than controls (P < 0.05). Toluidine blue and aqueous fast green, 20x magnification. Scale bar = 100 μm. * calcified cartilage; ** bone.

Mentions: Figure 2 shows growth plate cartilage of the proximal tibia in lean and obese mice. Articular chondrocytes were much more disorganized in ob/ob mice than lean mice in both the control and resveratrol-treated groups. Table 2 depicts results of the two-way ANOVA for tibial histomorphometric comparisons. Obese mice had significantly reduced Md.GPl.Ar and Tt.GPl.Ar in comparison to lean mice (F = 12.7; P < 0.01 and F = 3.9; P = 0.05 respectively). Mice treated with resveratrol exhibited a smaller Md.GPl.Ar than controls (F = 4.0; P = 0.05), as well as a reduced Md.GPl.Ar/Tt.GPl.Ar ratio (F = 5.0; P = 0.04).Figure 2


Effects of resveratrol treatment on bone and cartilage in obese diabetic mice.

Cooley J, Broderick TL, Al-Nakkash L, Plochocki JH - J Diabetes Metab Disord (2015)

Photo micrographs of the proximal tibial growth plate of lean control (A), lean resveratrol-treated (B), obese control (C), and obese resveratrol-treated (D) mice. Obese mice had growth plate chondrocytes that appeared to be more disorganized than those of lean mice. Mice treated with resveratrol had significantly greater calcified cartilage areas than controls (P < 0.05). Toluidine blue and aqueous fast green, 20x magnification. Scale bar = 100 μm. * calcified cartilage; ** bone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4363196&req=5

Fig2: Photo micrographs of the proximal tibial growth plate of lean control (A), lean resveratrol-treated (B), obese control (C), and obese resveratrol-treated (D) mice. Obese mice had growth plate chondrocytes that appeared to be more disorganized than those of lean mice. Mice treated with resveratrol had significantly greater calcified cartilage areas than controls (P < 0.05). Toluidine blue and aqueous fast green, 20x magnification. Scale bar = 100 μm. * calcified cartilage; ** bone.
Mentions: Figure 2 shows growth plate cartilage of the proximal tibia in lean and obese mice. Articular chondrocytes were much more disorganized in ob/ob mice than lean mice in both the control and resveratrol-treated groups. Table 2 depicts results of the two-way ANOVA for tibial histomorphometric comparisons. Obese mice had significantly reduced Md.GPl.Ar and Tt.GPl.Ar in comparison to lean mice (F = 12.7; P < 0.01 and F = 3.9; P = 0.05 respectively). Mice treated with resveratrol exhibited a smaller Md.GPl.Ar than controls (F = 4.0; P = 0.05), as well as a reduced Md.GPl.Ar/Tt.GPl.Ar ratio (F = 5.0; P = 0.04).Figure 2

Bottom Line: Resveratrol treatment significantly increased cortical area in both ob/ob and control mice, but did not improve cross-sectional indicators of resistance to bending.Resveratrol treatment of ob/ob mice had mixed effects on bone histomorphometry at the femoral midshaft.Treatment increased cortical area but decreased bone length.

View Article: PubMed Central - PubMed

Affiliation: Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ USA.

ABSTRACT

Background: Resveratrol is a polyphenolic phytoalexin that has been shown to exhibit osteoprotective and chondroprotective properties. We examine the effects of resveratrol treatment on bone and cartilage tissue of obese, diabetic ob/ob mice.

Methods: Eight-week-old ob/ob and lean control mice were given trans-resveratrol at an oral dose of 25 mg/kg for 3 weeks. Histomorphometric and cross-sectional-geometric variables were analyzed.

Results: Ob/ob mice in our study exhibit significantly reduced femoral length, resistance to loading, and tibial growth plate total area and calcified area than lean controls (P < 0.05). Resveratrol treatment significantly increased cortical area in both ob/ob and control mice, but did not improve cross-sectional indicators of resistance to bending. Resveratrol treatment also reduced tibial length and calcified growth plate cartilage area in comparison to untreated mice (P < 0.05).

Conclusion: Resveratrol treatment of ob/ob mice had mixed effects on bone histomorphometry at the femoral midshaft. Treatment increased cortical area but decreased bone length.

No MeSH data available.


Related in: MedlinePlus