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VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus

VEGF121 concentrations increase in the vitreous humor with advancing gestationBox-plots show VEGF121 concentrations in vitreous humor from fetuses of 10-14 weeks, 15-19 weeks, and 20-24 weeks. * indicates P<0.05.
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Figure 7: VEGF121 concentrations increase in the vitreous humor with advancing gestationBox-plots show VEGF121 concentrations in vitreous humor from fetuses of 10-14 weeks, 15-19 weeks, and 20-24 weeks. * indicates P<0.05.

Mentions: Existing information indicates that VEGF121 and VEGF165 are diffusible proteins, in contrast to the larger, tissue-bound VEGF189 protein (12). Therefore, to confirm the biological relevance of our findings at the protein level, we next measured VEGF121concentrations in vitreous humor from these fetuses. As shown in figure 7, VEGF121 concentrations increased in vitreous humor with advancing gestation. Compared to fetuses of 10-14 weeks' gestation, there was a trend towards higher vitreous VEGF121 concentrations at 15-19 weeks, and significantly higher VEGF121 concentrations at 20-24 weeks.


VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

VEGF121 concentrations increase in the vitreous humor with advancing gestationBox-plots show VEGF121 concentrations in vitreous humor from fetuses of 10-14 weeks, 15-19 weeks, and 20-24 weeks. * indicates P<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363168&req=5

Figure 7: VEGF121 concentrations increase in the vitreous humor with advancing gestationBox-plots show VEGF121 concentrations in vitreous humor from fetuses of 10-14 weeks, 15-19 weeks, and 20-24 weeks. * indicates P<0.05.
Mentions: Existing information indicates that VEGF121 and VEGF165 are diffusible proteins, in contrast to the larger, tissue-bound VEGF189 protein (12). Therefore, to confirm the biological relevance of our findings at the protein level, we next measured VEGF121concentrations in vitreous humor from these fetuses. As shown in figure 7, VEGF121 concentrations increased in vitreous humor with advancing gestation. Compared to fetuses of 10-14 weeks' gestation, there was a trend towards higher vitreous VEGF121 concentrations at 15-19 weeks, and significantly higher VEGF121 concentrations at 20-24 weeks.

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus