Limits...
VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus

VEGF-A splice variants in the midgestation fetal retinaTop: Schematic shows the complete pre-mRNA and the major splice variants of VEGF-A. The site of the primers is indicated by arrows. Bottom: Representative agarose gels showing PCR products corresponding to the major splice variants in fetal retina, brain, and colon.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4363168&req=5

Figure 5: VEGF-A splice variants in the midgestation fetal retinaTop: Schematic shows the complete pre-mRNA and the major splice variants of VEGF-A. The site of the primers is indicated by arrows. Bottom: Representative agarose gels showing PCR products corresponding to the major splice variants in fetal retina, brain, and colon.

Mentions: We next PCR-amplified major splice variants of VEGF-A mRNA in retinal tissue from fetuses of 20-24 weeks' gestation. As shown in figure 5, VEGF121was the predominant splice variant of VEGF-A, but we also detected faint bands of VEGF165 and VEGF189. These findings were specific to the retina; we detected only VEGF121and VEGF206 in the fetal brain, but all the 5 splice variants in the fetal colon at the same gestational age.


VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

VEGF-A splice variants in the midgestation fetal retinaTop: Schematic shows the complete pre-mRNA and the major splice variants of VEGF-A. The site of the primers is indicated by arrows. Bottom: Representative agarose gels showing PCR products corresponding to the major splice variants in fetal retina, brain, and colon.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4363168&req=5

Figure 5: VEGF-A splice variants in the midgestation fetal retinaTop: Schematic shows the complete pre-mRNA and the major splice variants of VEGF-A. The site of the primers is indicated by arrows. Bottom: Representative agarose gels showing PCR products corresponding to the major splice variants in fetal retina, brain, and colon.
Mentions: We next PCR-amplified major splice variants of VEGF-A mRNA in retinal tissue from fetuses of 20-24 weeks' gestation. As shown in figure 5, VEGF121was the predominant splice variant of VEGF-A, but we also detected faint bands of VEGF165 and VEGF189. These findings were specific to the retina; we detected only VEGF121and VEGF206 in the fetal brain, but all the 5 splice variants in the fetal colon at the same gestational age.

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus