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VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus

Tissue-bound VEGF-A protein expression in retinal tissue from fetuses at 10-14 weeks and 20-24 weeks gestationRepresentative Western blots show tissue-bound VEGF-A and β-actin. Bar-diagrams (means ± SE) summarize densitometric data.
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Figure 4: Tissue-bound VEGF-A protein expression in retinal tissue from fetuses at 10-14 weeks and 20-24 weeks gestationRepresentative Western blots show tissue-bound VEGF-A and β-actin. Bar-diagrams (means ± SE) summarize densitometric data.

Mentions: We measured mRNA expression of VEGF-A, -B, -C, and -D isoforms in fetal retinal tissue by RT-qPCR. As shown in figure 3, the expression of VEGF-A, but not VEGF-B, -C, or D, increased in the midgestation retina as a function of gestation age. VEGF mRNA expression showed a strong positive correlation with gestational age (inset; Spearman's r = 0.77, p<0.05). Consistent with these findings, we measured VEGF in retinal tissue and showed higher VEGF-A protein expression in tissue from 20-24 week fetuses than 11-14 week fetuses (figure 4).


VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

Tissue-bound VEGF-A protein expression in retinal tissue from fetuses at 10-14 weeks and 20-24 weeks gestationRepresentative Western blots show tissue-bound VEGF-A and β-actin. Bar-diagrams (means ± SE) summarize densitometric data.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4363168&req=5

Figure 4: Tissue-bound VEGF-A protein expression in retinal tissue from fetuses at 10-14 weeks and 20-24 weeks gestationRepresentative Western blots show tissue-bound VEGF-A and β-actin. Bar-diagrams (means ± SE) summarize densitometric data.
Mentions: We measured mRNA expression of VEGF-A, -B, -C, and -D isoforms in fetal retinal tissue by RT-qPCR. As shown in figure 3, the expression of VEGF-A, but not VEGF-B, -C, or D, increased in the midgestation retina as a function of gestation age. VEGF mRNA expression showed a strong positive correlation with gestational age (inset; Spearman's r = 0.77, p<0.05). Consistent with these findings, we measured VEGF in retinal tissue and showed higher VEGF-A protein expression in tissue from 20-24 week fetuses than 11-14 week fetuses (figure 4).

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus