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VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus

Gene expression of GAPDH, β-actin, 18s and HIF-1αGene expression in retina for GAPDH (panel A), 18s (panel B) β-actin (panel C), and HIF-1α (panel D) from 10-24 week samples is shown. Gene expression of GAPDH, β-actin and HIF-1a was not significantly different across the range of gestational age tested, while gene expression of 18s increased with increasing gestational age (p=0.006).
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Figure 1: Gene expression of GAPDH, β-actin, 18s and HIF-1αGene expression in retina for GAPDH (panel A), 18s (panel B) β-actin (panel C), and HIF-1α (panel D) from 10-24 week samples is shown. Gene expression of GAPDH, β-actin and HIF-1a was not significantly different across the range of gestational age tested, while gene expression of 18s increased with increasing gestational age (p=0.006).

Mentions: We evaluated beta-actin (β-actin), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxia inducible factor 1 alpha (HIF-1α) and ribosomal 18s as possible genes to serve as normalizing controls in quantitative PCR (qPCR) reactions (figure 1). We found no statistical difference in gene expression across all gestational ages tested (10-24 weeks) with GAPDH (p=0.108), β-actin (p=0.522) or HIF-1α (p=0.077), while 18s gene expression increased significantly from early to late mid-gestation with 18s (p=0.006). We chose GAPDH to serve as our endogenous control because of its relative abundance in relation to VEGF.


VEGF mRNA and protein concentrations in the developing human eye.

Ma IT, McConaghy S, Namachivayam K, Halloran BA, Kurundkar AR, MohanKumar K, Maheshwari A, Ohls RK - Pediatr. Res. (2015)

Gene expression of GAPDH, β-actin, 18s and HIF-1αGene expression in retina for GAPDH (panel A), 18s (panel B) β-actin (panel C), and HIF-1α (panel D) from 10-24 week samples is shown. Gene expression of GAPDH, β-actin and HIF-1a was not significantly different across the range of gestational age tested, while gene expression of 18s increased with increasing gestational age (p=0.006).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363168&req=5

Figure 1: Gene expression of GAPDH, β-actin, 18s and HIF-1αGene expression in retina for GAPDH (panel A), 18s (panel B) β-actin (panel C), and HIF-1α (panel D) from 10-24 week samples is shown. Gene expression of GAPDH, β-actin and HIF-1a was not significantly different across the range of gestational age tested, while gene expression of 18s increased with increasing gestational age (p=0.006).
Mentions: We evaluated beta-actin (β-actin), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxia inducible factor 1 alpha (HIF-1α) and ribosomal 18s as possible genes to serve as normalizing controls in quantitative PCR (qPCR) reactions (figure 1). We found no statistical difference in gene expression across all gestational ages tested (10-24 weeks) with GAPDH (p=0.108), β-actin (p=0.522) or HIF-1α (p=0.077), while 18s gene expression increased significantly from early to late mid-gestation with 18s (p=0.006). We chose GAPDH to serve as our endogenous control because of its relative abundance in relation to VEGF.

Bottom Line: VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation.Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Mayo Clinic Arizona, Phoenix, Arizona.

ABSTRACT

Background: Vascular endothelial growth factor (VEGF), a well-characterized regulator of angiogenesis, has been mechanistically implicated in retinal neovascularization and in the pathogenesis of retinopathy of prematurity. However, the ontogeny of VEGF expression in the human fetal retina is not well known. Because retinal vasculature grows with gestational maturation, we hypothesized that VEGF expression also increases in the midgestation human fetal eye as a function of gestational age.

Methods: To identify changes in VEGF gene expression during normal human development, we measured VEGF mRNA by quantitative PCR and measured VEGF protein by enzyme-linked immunosorbent assay and western blots in 10-24 wk gestation fetal vitreous, retina, and serum.

Results: VEGF mRNA expression in the retina increased with gestational age. VEGF isoform A, particularly its VEGF121 splice variant, contributed to this positive correlation. Consistent with these findings, we detected increasing VEGF121 protein concentrations in vitreous humor from fetuses of 10-24 wk gestation, while VEGF concentrations decreased in fetal serum.

Conclusion: VEGF121 mRNA and protein concentrations increase with increasing gestational age in the developing human retina. We speculate that VEGF plays an important role in normal retinal vascular development, and that preterm delivery affects production of this vascular growth factor.

No MeSH data available.


Related in: MedlinePlus