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A single pair of neurons modulates egg-laying decisions in Drosophila.

Wu CL, Fu TF, Chou YY, Yeh SR - PLoS ONE (2015)

Bottom Line: In the present study, we found that amnesiac (amn) mutant flies show significant defects in egg-laying decisions, and such defects can be reversed by expressing the wild-type amn transgene in two dorsal paired medial (DPM) neurons in the brain.Finally, the activity in mushroom body αβ neurons is required for the egg-laying behavior, suggesting a possible "DPM-αβ neurons" brain circuit modulating egg-laying decisions.Our results highlight the brain circuits and molecular mechanisms of egg-laying decisions in Drosophila.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Medical Research, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

ABSTRACT
Animals have to judge environmental cues and choose the most suitable option for them from many different options. Female fruit flies selecting an optimum site to deposit their eggs is a biologically important reproductive behavior. When given the direct choice between ovipositing their eggs in a sucrose-containing medium or a caffeine-containing medium, female flies prefer the latter. However, the neural circuits and molecules that regulate this decision-making processes during egg-laying site selection remain poorly understood. In the present study, we found that amnesiac (amn) mutant flies show significant defects in egg-laying decisions, and such defects can be reversed by expressing the wild-type amn transgene in two dorsal paired medial (DPM) neurons in the brain. Silencing neuronal activity with an inward rectifier potassium channel (Kir2.1) in DPM neurons also impairs egg-laying decisions. Finally, the activity in mushroom body αβ neurons is required for the egg-laying behavior, suggesting a possible "DPM-αβ neurons" brain circuit modulating egg-laying decisions. Our results highlight the brain circuits and molecular mechanisms of egg-laying decisions in Drosophila.

No MeSH data available.


Related in: MedlinePlus

Aging does not alter Drosophila egg-laying decisions.(A) Schematic representation of the chamber for egg-laying decisions assays. Two 1% agarose media, containing either 100 mM sucrose (orange) or 100 mM caffeine (blue), were loaded above the 3% hard agarose medium (grey). A gap (0.1 cm) was made on the 3% agarose medium in the middle of the chamber. (B) Egg-laying decisions of wild-type flies at different ages (5-day and 21-day). Each value represents mean ± SEM (N = 36–42, n.s., not statistically significant).
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pone.0121335.g001: Aging does not alter Drosophila egg-laying decisions.(A) Schematic representation of the chamber for egg-laying decisions assays. Two 1% agarose media, containing either 100 mM sucrose (orange) or 100 mM caffeine (blue), were loaded above the 3% hard agarose medium (grey). A gap (0.1 cm) was made on the 3% agarose medium in the middle of the chamber. (B) Egg-laying decisions of wild-type flies at different ages (5-day and 21-day). Each value represents mean ± SEM (N = 36–42, n.s., not statistically significant).

Mentions: It has been reported that female flies avoid laying eggs on a medium containing sucrose, and that this egg-laying site selection relies on a simple decision-based behavioral process [1]. We took advantage of this behavioral assay to explore the molecules and brain circuits underlying egg-laying decisions. A plastic egg-laying chamber was placed in a sweet (sucrose-containing) and a bitter (caffeine-containing) 1% soft agarose medium, and the two media were separated by a region of 3% hard agarose, which contained a small gap in the middle to prevent diffusion of sucrose or caffeine to the opposite site [2](Fig. 1A). Consistent with the previous findings, female flies prefer to deposit their eggs on a bitter substrate (Fig. 1B)[1]. Decision-making processes involve neuronal function in the brain, and aging significantly alters the functioning of the nervous system [19,20]. We first tested whether egg-laying decisions changed with aging. The 21-day old female flies were used for the behavioral assays [21], and we found that aged flies still executed normal egg-laying decisions compared to young flies (5-day old) (Fig. 1B).


A single pair of neurons modulates egg-laying decisions in Drosophila.

Wu CL, Fu TF, Chou YY, Yeh SR - PLoS ONE (2015)

Aging does not alter Drosophila egg-laying decisions.(A) Schematic representation of the chamber for egg-laying decisions assays. Two 1% agarose media, containing either 100 mM sucrose (orange) or 100 mM caffeine (blue), were loaded above the 3% hard agarose medium (grey). A gap (0.1 cm) was made on the 3% agarose medium in the middle of the chamber. (B) Egg-laying decisions of wild-type flies at different ages (5-day and 21-day). Each value represents mean ± SEM (N = 36–42, n.s., not statistically significant).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363143&req=5

pone.0121335.g001: Aging does not alter Drosophila egg-laying decisions.(A) Schematic representation of the chamber for egg-laying decisions assays. Two 1% agarose media, containing either 100 mM sucrose (orange) or 100 mM caffeine (blue), were loaded above the 3% hard agarose medium (grey). A gap (0.1 cm) was made on the 3% agarose medium in the middle of the chamber. (B) Egg-laying decisions of wild-type flies at different ages (5-day and 21-day). Each value represents mean ± SEM (N = 36–42, n.s., not statistically significant).
Mentions: It has been reported that female flies avoid laying eggs on a medium containing sucrose, and that this egg-laying site selection relies on a simple decision-based behavioral process [1]. We took advantage of this behavioral assay to explore the molecules and brain circuits underlying egg-laying decisions. A plastic egg-laying chamber was placed in a sweet (sucrose-containing) and a bitter (caffeine-containing) 1% soft agarose medium, and the two media were separated by a region of 3% hard agarose, which contained a small gap in the middle to prevent diffusion of sucrose or caffeine to the opposite site [2](Fig. 1A). Consistent with the previous findings, female flies prefer to deposit their eggs on a bitter substrate (Fig. 1B)[1]. Decision-making processes involve neuronal function in the brain, and aging significantly alters the functioning of the nervous system [19,20]. We first tested whether egg-laying decisions changed with aging. The 21-day old female flies were used for the behavioral assays [21], and we found that aged flies still executed normal egg-laying decisions compared to young flies (5-day old) (Fig. 1B).

Bottom Line: In the present study, we found that amnesiac (amn) mutant flies show significant defects in egg-laying decisions, and such defects can be reversed by expressing the wild-type amn transgene in two dorsal paired medial (DPM) neurons in the brain.Finally, the activity in mushroom body αβ neurons is required for the egg-laying behavior, suggesting a possible "DPM-αβ neurons" brain circuit modulating egg-laying decisions.Our results highlight the brain circuits and molecular mechanisms of egg-laying decisions in Drosophila.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Medical Research, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

ABSTRACT
Animals have to judge environmental cues and choose the most suitable option for them from many different options. Female fruit flies selecting an optimum site to deposit their eggs is a biologically important reproductive behavior. When given the direct choice between ovipositing their eggs in a sucrose-containing medium or a caffeine-containing medium, female flies prefer the latter. However, the neural circuits and molecules that regulate this decision-making processes during egg-laying site selection remain poorly understood. In the present study, we found that amnesiac (amn) mutant flies show significant defects in egg-laying decisions, and such defects can be reversed by expressing the wild-type amn transgene in two dorsal paired medial (DPM) neurons in the brain. Silencing neuronal activity with an inward rectifier potassium channel (Kir2.1) in DPM neurons also impairs egg-laying decisions. Finally, the activity in mushroom body αβ neurons is required for the egg-laying behavior, suggesting a possible "DPM-αβ neurons" brain circuit modulating egg-laying decisions. Our results highlight the brain circuits and molecular mechanisms of egg-laying decisions in Drosophila.

No MeSH data available.


Related in: MedlinePlus