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Samsoeum water extract attenuates allergic airway inflammation via modulation of Th1/Th2 cytokines and decrease of iNOS expression in asthmatic mice.

Jeon WY, Shin IS, Shin HK, Lee MY - BMC Complement Altern Med (2015)

Bottom Line: Female BALB/c mice were sensitized by an intraperitoneal injection of OVA and subsequently challenged with nebulized OVA.Our findings indicated that SSEW decreased the accumulation of inflammatory cells (particularly, eosinophil and neutrophil) and regulated the balance in the production of Th1/Th2 cytokines and chemokine in BALF.Collectively, these results suggest that, because of its anti-inflammatory and antiasthma properties, SSEW may be useful in reducing airway inflammation in the treatment of allergic asthma.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon, 305-811, Republic of Korea. ssamggun85@kiom.re.kr.

ABSTRACT

Background: Samsoeum has long been used in Korea and other Asian countries as a traditional medicine to treat various diseases. In the present study, we investigated the antiasthma effect of the herbal medicine Samsoeum water extract (SSEW) using an in vivo ovalbumin (OVA)-induced asthmatic model.

Methods: Female BALB/c mice were sensitized by an intraperitoneal injection of OVA and subsequently challenged with nebulized OVA. We investigated the number of inflammatory cells, the production of Th1/Th2 cytokines and chemokine in bronchoalveolar lavage fluid (BALF), histological changes in lung tissue, the infiltration of inflammatory cells and hyperplasia of goblet cells in lung tissue, the levels of immunoglobulin E (IgE) in BALF and plasma, and the expression of inducible nitric oxide synthase (iNOS) in lung tissue.

Results: Our findings indicated that SSEW decreased the accumulation of inflammatory cells (particularly, eosinophil and neutrophil) and regulated the balance in the production of Th1/Th2 cytokines and chemokine in BALF. Moreover, SSEW suppressed the level of IgE in BALF and plasma, and inhibited the infiltration of inflammatory cells, hyperplasia of goblet cells, and the expression of iNOS in lung tissue.

Conclusions: Collectively, these results suggest that, because of its anti-inflammatory and antiasthma properties, SSEW may be useful in reducing airway inflammation in the treatment of allergic asthma.

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Related in: MedlinePlus

Effect of SSEW on Th2 cytokines in the BALF of OVA-induced mice. Individual data were obtained using ELISA.(A) IL-4, (B) IL-5, (C) IL-10, (D) IL-13, and (E) IL-33. NC, normal control group (vehicle); OVA, OVA-induced group (control); Mon, montelukast (30 mg/kg) + OVA-induced group (positive control); SSEW-100, SSEW (100 mg/kg) + OVA-induced group; SSEW-200, SSEW (200 mg/kg) + OVA-induced group. The values represent the mean ± S.E.M (n = 6/group). Significant differences at ##P < 0.01 compared with the NC group. Significant differences at *P < 0.05 and **P < 0.01 compared with the OVA-induced group.
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Fig4: Effect of SSEW on Th2 cytokines in the BALF of OVA-induced mice. Individual data were obtained using ELISA.(A) IL-4, (B) IL-5, (C) IL-10, (D) IL-13, and (E) IL-33. NC, normal control group (vehicle); OVA, OVA-induced group (control); Mon, montelukast (30 mg/kg) + OVA-induced group (positive control); SSEW-100, SSEW (100 mg/kg) + OVA-induced group; SSEW-200, SSEW (200 mg/kg) + OVA-induced group. The values represent the mean ± S.E.M (n = 6/group). Significant differences at ##P < 0.01 compared with the NC group. Significant differences at *P < 0.05 and **P < 0.01 compared with the OVA-induced group.

Mentions: To confirm the association between SSEW and Th2 responses in BALF from OVA-induced asthmatic mice, we assessed the levels of Th2-type cytokines in BALF. Figure 4 shows that the OVA group exhibited significantly elevated levels of Th2-type cytokines, including (A) IL-4, (B) IL-5, (C) IL-10, (D) IL-13, and (E) IL-33, compared with the NC group. However, the SSEW groups (100 and 200 mg/kg) showed significantly decreased levels of Th2 cytokines compared with the OVA group, similar to the Mon group (Figure 4A–E).Figure 4


Samsoeum water extract attenuates allergic airway inflammation via modulation of Th1/Th2 cytokines and decrease of iNOS expression in asthmatic mice.

Jeon WY, Shin IS, Shin HK, Lee MY - BMC Complement Altern Med (2015)

Effect of SSEW on Th2 cytokines in the BALF of OVA-induced mice. Individual data were obtained using ELISA.(A) IL-4, (B) IL-5, (C) IL-10, (D) IL-13, and (E) IL-33. NC, normal control group (vehicle); OVA, OVA-induced group (control); Mon, montelukast (30 mg/kg) + OVA-induced group (positive control); SSEW-100, SSEW (100 mg/kg) + OVA-induced group; SSEW-200, SSEW (200 mg/kg) + OVA-induced group. The values represent the mean ± S.E.M (n = 6/group). Significant differences at ##P < 0.01 compared with the NC group. Significant differences at *P < 0.05 and **P < 0.01 compared with the OVA-induced group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4363060&req=5

Fig4: Effect of SSEW on Th2 cytokines in the BALF of OVA-induced mice. Individual data were obtained using ELISA.(A) IL-4, (B) IL-5, (C) IL-10, (D) IL-13, and (E) IL-33. NC, normal control group (vehicle); OVA, OVA-induced group (control); Mon, montelukast (30 mg/kg) + OVA-induced group (positive control); SSEW-100, SSEW (100 mg/kg) + OVA-induced group; SSEW-200, SSEW (200 mg/kg) + OVA-induced group. The values represent the mean ± S.E.M (n = 6/group). Significant differences at ##P < 0.01 compared with the NC group. Significant differences at *P < 0.05 and **P < 0.01 compared with the OVA-induced group.
Mentions: To confirm the association between SSEW and Th2 responses in BALF from OVA-induced asthmatic mice, we assessed the levels of Th2-type cytokines in BALF. Figure 4 shows that the OVA group exhibited significantly elevated levels of Th2-type cytokines, including (A) IL-4, (B) IL-5, (C) IL-10, (D) IL-13, and (E) IL-33, compared with the NC group. However, the SSEW groups (100 and 200 mg/kg) showed significantly decreased levels of Th2 cytokines compared with the OVA group, similar to the Mon group (Figure 4A–E).Figure 4

Bottom Line: Female BALB/c mice were sensitized by an intraperitoneal injection of OVA and subsequently challenged with nebulized OVA.Our findings indicated that SSEW decreased the accumulation of inflammatory cells (particularly, eosinophil and neutrophil) and regulated the balance in the production of Th1/Th2 cytokines and chemokine in BALF.Collectively, these results suggest that, because of its anti-inflammatory and antiasthma properties, SSEW may be useful in reducing airway inflammation in the treatment of allergic asthma.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon, 305-811, Republic of Korea. ssamggun85@kiom.re.kr.

ABSTRACT

Background: Samsoeum has long been used in Korea and other Asian countries as a traditional medicine to treat various diseases. In the present study, we investigated the antiasthma effect of the herbal medicine Samsoeum water extract (SSEW) using an in vivo ovalbumin (OVA)-induced asthmatic model.

Methods: Female BALB/c mice were sensitized by an intraperitoneal injection of OVA and subsequently challenged with nebulized OVA. We investigated the number of inflammatory cells, the production of Th1/Th2 cytokines and chemokine in bronchoalveolar lavage fluid (BALF), histological changes in lung tissue, the infiltration of inflammatory cells and hyperplasia of goblet cells in lung tissue, the levels of immunoglobulin E (IgE) in BALF and plasma, and the expression of inducible nitric oxide synthase (iNOS) in lung tissue.

Results: Our findings indicated that SSEW decreased the accumulation of inflammatory cells (particularly, eosinophil and neutrophil) and regulated the balance in the production of Th1/Th2 cytokines and chemokine in BALF. Moreover, SSEW suppressed the level of IgE in BALF and plasma, and inhibited the infiltration of inflammatory cells, hyperplasia of goblet cells, and the expression of iNOS in lung tissue.

Conclusions: Collectively, these results suggest that, because of its anti-inflammatory and antiasthma properties, SSEW may be useful in reducing airway inflammation in the treatment of allergic asthma.

Show MeSH
Related in: MedlinePlus