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Cannabidiol, a non-psychoactive cannabinoid, leads to EGR2-dependent anergy in activated encephalitogenic T cells.

Kozela E, Juknat A, Kaushansky N, Ben-Nun A, Coppola G, Vogel Z - J Neuroinflammation (2015)

Bottom Line: In parallel, we observed decreased levels of major histocompatibility complex class II (MHCII), CD25, and CD69 on CD19(+) B cells following CBD treatment, showing diminished antigen presenting capabilities of B cells and reduction in their pro-inflammatory functions.Our data suggests that CBD exerts its immunoregulatory effects via induction of CD4(+)CD25(-)CD69(+)LAG3(+) cells in MOG35-55-activated APC/TMOG co-cultures.This is accompanied by EGR2-dependent anergy of stimulated TMOG cells as well as a switch in their intracellular STAT3/STAT5 activation balance leading to the previously observed decrease in Th17 activity.

View Article: PubMed Central - PubMed

Affiliation: The Dr Miriam and Sheldon G. Adelson Center for the Biology of Addictive Diseases, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. ewa.kozela@weizmann.ac.il.

ABSTRACT

Background: Cannabidiol (CBD), the main non-psychoactive cannabinoid, has been previously shown by us to ameliorate clinical symptoms and to decrease inflammation in myelin oligodendrocyte glycoprotein (MOG)35-55-induced mouse experimental autoimmune encephalomyelitis model of multiple sclerosis as well as to decrease MOG35-55-induced T cell proliferation and IL-17 secretion. However, the mechanisms of CBD anti-inflammatory activities are unclear.

Methods: Here we analyzed the effects of CBD on splenocytes (source of accessory T cells and antigen presenting cells (APC)) co-cultured with MOG35-55-specific T cells (TMOG) and stimulated with MOG35-55. Using flow cytometry, we evaluated the expression of surface activation markers and inhibitory molecules on T cells and B cells. TMOG cells were purified using CD4 positive microbead selection and submitted for quantitative PCR and microarray of mRNA transcript analyzes. Cell signaling studies in purified TMOG were carried out using immunoblotting.

Results: We found that CBD leads to upregulation of CD69 and lymphocyte-activation gene 3 (LAG3) regulatory molecules on CD4(+)CD25(-) accessory T cells. This subtype of CD4(+)CD25(-)CD69(+)LAG3(+) T cells has been recognized as induced regulatory phenotype promoting anergy in activated T cells. Indeed, we observed that CBD treatment results in upregulation of EGR2 (a key T cell anergy inducer) mRNA transcription in stimulated TMOG cells. This was accompanied by elevated levels of anergy promoting genes such as IL-10 (anti-inflammatory cytokine), STAT5 (regulatory factor), and LAG3 mRNAs, as well as of several enhancers of cell cycle arrest (such as Nfatc1, Casp4, Cdkn1a, and Icos). Moreover, CBD exposure leads to a decrease in STAT3 and to an increase in STAT5 phosphorylation in TMOG cells, positive and negative regulators of Th17 activity, respectively. In parallel, we observed decreased levels of major histocompatibility complex class II (MHCII), CD25, and CD69 on CD19(+) B cells following CBD treatment, showing diminished antigen presenting capabilities of B cells and reduction in their pro-inflammatory functions.

Conclusions: Our data suggests that CBD exerts its immunoregulatory effects via induction of CD4(+)CD25(-)CD69(+)LAG3(+) cells in MOG35-55-activated APC/TMOG co-cultures. This is accompanied by EGR2-dependent anergy of stimulated TMOG cells as well as a switch in their intracellular STAT3/STAT5 activation balance leading to the previously observed decrease in Th17 activity.

No MeSH data available.


Related in: MedlinePlus

MOG35-55 increases and CBD decreases the number of CD19+CD69+B cells in APC/TMOGco-cultures. APC/TMOG co-cultures were examined for CD19+CD69+ cells using flow cytometry. (A) Dot plot graph showing gating for CD19+CD69+ cells; (B) representative dot plots showing flow cytometry analysis of CD69 expression on CD19+ B cells with a quadrant marking CD19highCD69high cells; (C) bar graph showing the mean percentages ± SEM of CD19highCD69high within CD19+CD69+ parent population (equivalent to 100%). ANOVA F(3,8) = 19.3, P < 0.001. **P < 0.01 vs control, ##P < 0.01 vs MOG-stimulated cells. n = 3.
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Fig7: MOG35-55 increases and CBD decreases the number of CD19+CD69+B cells in APC/TMOGco-cultures. APC/TMOG co-cultures were examined for CD19+CD69+ cells using flow cytometry. (A) Dot plot graph showing gating for CD19+CD69+ cells; (B) representative dot plots showing flow cytometry analysis of CD69 expression on CD19+ B cells with a quadrant marking CD19highCD69high cells; (C) bar graph showing the mean percentages ± SEM of CD19highCD69high within CD19+CD69+ parent population (equivalent to 100%). ANOVA F(3,8) = 19.3, P < 0.001. **P < 0.01 vs control, ##P < 0.01 vs MOG-stimulated cells. n = 3.

Mentions: CD69 level on CD19+ B cells serves as an indication of B cells pro-inflammatory activity. In spleen-derived APC cultured without TMOG cells (resting splenocytes), 13.6% ± 3.4% of the CD19+ B cells are CD69 positive and this expression is not significantly affected by CBD treatment (17.4% ± 3.4%; Table 2). However, co-culturing of spleen-derived APC cells with TMOG cells resulted in a remarkable increase in CD69 antigen, particularly on CD19high B cells as this population reached 44.2% ± 2.6% of all CD19+CD69+ cells (Figure 7B,C). CBD co-incubation decreased the number of CD19highCD69high in non-stimulated APC/TMOG co-cultures down to 21.6% ± 5.9% (P < 0.01). MOG35-55 stimulation of APC/T co-cultures resulted in a further significant increase in CD19highCD69high frequency reaching 70.0% ± 1.0% of the total number of CD19+ cells (P < 0.01). This increase was reduced in the presence of CBD to 45.2% ± 6.9% - a reduction similar to that observed in non-stimulated APC/TMOG co-cultures (Figure 7B,C).Figure 7


Cannabidiol, a non-psychoactive cannabinoid, leads to EGR2-dependent anergy in activated encephalitogenic T cells.

Kozela E, Juknat A, Kaushansky N, Ben-Nun A, Coppola G, Vogel Z - J Neuroinflammation (2015)

MOG35-55 increases and CBD decreases the number of CD19+CD69+B cells in APC/TMOGco-cultures. APC/TMOG co-cultures were examined for CD19+CD69+ cells using flow cytometry. (A) Dot plot graph showing gating for CD19+CD69+ cells; (B) representative dot plots showing flow cytometry analysis of CD69 expression on CD19+ B cells with a quadrant marking CD19highCD69high cells; (C) bar graph showing the mean percentages ± SEM of CD19highCD69high within CD19+CD69+ parent population (equivalent to 100%). ANOVA F(3,8) = 19.3, P < 0.001. **P < 0.01 vs control, ##P < 0.01 vs MOG-stimulated cells. n = 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4363052&req=5

Fig7: MOG35-55 increases and CBD decreases the number of CD19+CD69+B cells in APC/TMOGco-cultures. APC/TMOG co-cultures were examined for CD19+CD69+ cells using flow cytometry. (A) Dot plot graph showing gating for CD19+CD69+ cells; (B) representative dot plots showing flow cytometry analysis of CD69 expression on CD19+ B cells with a quadrant marking CD19highCD69high cells; (C) bar graph showing the mean percentages ± SEM of CD19highCD69high within CD19+CD69+ parent population (equivalent to 100%). ANOVA F(3,8) = 19.3, P < 0.001. **P < 0.01 vs control, ##P < 0.01 vs MOG-stimulated cells. n = 3.
Mentions: CD69 level on CD19+ B cells serves as an indication of B cells pro-inflammatory activity. In spleen-derived APC cultured without TMOG cells (resting splenocytes), 13.6% ± 3.4% of the CD19+ B cells are CD69 positive and this expression is not significantly affected by CBD treatment (17.4% ± 3.4%; Table 2). However, co-culturing of spleen-derived APC cells with TMOG cells resulted in a remarkable increase in CD69 antigen, particularly on CD19high B cells as this population reached 44.2% ± 2.6% of all CD19+CD69+ cells (Figure 7B,C). CBD co-incubation decreased the number of CD19highCD69high in non-stimulated APC/TMOG co-cultures down to 21.6% ± 5.9% (P < 0.01). MOG35-55 stimulation of APC/T co-cultures resulted in a further significant increase in CD19highCD69high frequency reaching 70.0% ± 1.0% of the total number of CD19+ cells (P < 0.01). This increase was reduced in the presence of CBD to 45.2% ± 6.9% - a reduction similar to that observed in non-stimulated APC/TMOG co-cultures (Figure 7B,C).Figure 7

Bottom Line: In parallel, we observed decreased levels of major histocompatibility complex class II (MHCII), CD25, and CD69 on CD19(+) B cells following CBD treatment, showing diminished antigen presenting capabilities of B cells and reduction in their pro-inflammatory functions.Our data suggests that CBD exerts its immunoregulatory effects via induction of CD4(+)CD25(-)CD69(+)LAG3(+) cells in MOG35-55-activated APC/TMOG co-cultures.This is accompanied by EGR2-dependent anergy of stimulated TMOG cells as well as a switch in their intracellular STAT3/STAT5 activation balance leading to the previously observed decrease in Th17 activity.

View Article: PubMed Central - PubMed

Affiliation: The Dr Miriam and Sheldon G. Adelson Center for the Biology of Addictive Diseases, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. ewa.kozela@weizmann.ac.il.

ABSTRACT

Background: Cannabidiol (CBD), the main non-psychoactive cannabinoid, has been previously shown by us to ameliorate clinical symptoms and to decrease inflammation in myelin oligodendrocyte glycoprotein (MOG)35-55-induced mouse experimental autoimmune encephalomyelitis model of multiple sclerosis as well as to decrease MOG35-55-induced T cell proliferation and IL-17 secretion. However, the mechanisms of CBD anti-inflammatory activities are unclear.

Methods: Here we analyzed the effects of CBD on splenocytes (source of accessory T cells and antigen presenting cells (APC)) co-cultured with MOG35-55-specific T cells (TMOG) and stimulated with MOG35-55. Using flow cytometry, we evaluated the expression of surface activation markers and inhibitory molecules on T cells and B cells. TMOG cells were purified using CD4 positive microbead selection and submitted for quantitative PCR and microarray of mRNA transcript analyzes. Cell signaling studies in purified TMOG were carried out using immunoblotting.

Results: We found that CBD leads to upregulation of CD69 and lymphocyte-activation gene 3 (LAG3) regulatory molecules on CD4(+)CD25(-) accessory T cells. This subtype of CD4(+)CD25(-)CD69(+)LAG3(+) T cells has been recognized as induced regulatory phenotype promoting anergy in activated T cells. Indeed, we observed that CBD treatment results in upregulation of EGR2 (a key T cell anergy inducer) mRNA transcription in stimulated TMOG cells. This was accompanied by elevated levels of anergy promoting genes such as IL-10 (anti-inflammatory cytokine), STAT5 (regulatory factor), and LAG3 mRNAs, as well as of several enhancers of cell cycle arrest (such as Nfatc1, Casp4, Cdkn1a, and Icos). Moreover, CBD exposure leads to a decrease in STAT3 and to an increase in STAT5 phosphorylation in TMOG cells, positive and negative regulators of Th17 activity, respectively. In parallel, we observed decreased levels of major histocompatibility complex class II (MHCII), CD25, and CD69 on CD19(+) B cells following CBD treatment, showing diminished antigen presenting capabilities of B cells and reduction in their pro-inflammatory functions.

Conclusions: Our data suggests that CBD exerts its immunoregulatory effects via induction of CD4(+)CD25(-)CD69(+)LAG3(+) cells in MOG35-55-activated APC/TMOG co-cultures. This is accompanied by EGR2-dependent anergy of stimulated TMOG cells as well as a switch in their intracellular STAT3/STAT5 activation balance leading to the previously observed decrease in Th17 activity.

No MeSH data available.


Related in: MedlinePlus