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Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa.

Nguyen TT, Motsiri MS, Taioe MO, Mtshali MS, Goto Y, Kawazu S, Thekisoe OM, Inoue N - J. Vet. Med. Sci. (2014)

Bottom Line: Out of 9 sheep serum samples, 2-4 sera (22.2-44.4%) were positive.The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6-0.7); both are recommended for reference diagnosis and large scale epidemiological surveys.There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.

View Article: PubMed Central - PubMed

Affiliation: OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

ABSTRACT
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8-46.3% and 0-9.1%, respectively. Out of 9 sheep serum samples, 2-4 sera (22.2-44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6-0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.

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Related in: MedlinePlus

Maps showing the sampled area. (A) South Africa with its nine provinces. (B) KwaZulu-Natal Province. (C) Umkhanyakude district with its five local municipalities. Samples were collected in Hlabisa, Mtubatuba and The Big 5 False Bay local municipalities.
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fig_001: Maps showing the sampled area. (A) South Africa with its nine provinces. (B) KwaZulu-Natal Province. (C) Umkhanyakude district with its five local municipalities. Samples were collected in Hlabisa, Mtubatuba and The Big 5 False Bay local municipalities.

Mentions: Blood sample collection was conducted out in 3 local municipalities of Umkhanyakude district in northeastern KwaZulu-Natal Province, namely Hlabisa (28.1333° S, 31.8667° E), Mtubatuba (28.4167° S, 32.1833° E) and The Big 5 False Bay (28.0189° S, 32.2675° E) between March and May 2013 (Fig. 1Fig. 1.


Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa.

Nguyen TT, Motsiri MS, Taioe MO, Mtshali MS, Goto Y, Kawazu S, Thekisoe OM, Inoue N - J. Vet. Med. Sci. (2014)

Maps showing the sampled area. (A) South Africa with its nine provinces. (B) KwaZulu-Natal Province. (C) Umkhanyakude district with its five local municipalities. Samples were collected in Hlabisa, Mtubatuba and The Big 5 False Bay local municipalities.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363025&req=5

fig_001: Maps showing the sampled area. (A) South Africa with its nine provinces. (B) KwaZulu-Natal Province. (C) Umkhanyakude district with its five local municipalities. Samples were collected in Hlabisa, Mtubatuba and The Big 5 False Bay local municipalities.
Mentions: Blood sample collection was conducted out in 3 local municipalities of Umkhanyakude district in northeastern KwaZulu-Natal Province, namely Hlabisa (28.1333° S, 31.8667° E), Mtubatuba (28.4167° S, 32.1833° E) and The Big 5 False Bay (28.0189° S, 32.2675° E) between March and May 2013 (Fig. 1Fig. 1.

Bottom Line: Out of 9 sheep serum samples, 2-4 sera (22.2-44.4%) were positive.The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6-0.7); both are recommended for reference diagnosis and large scale epidemiological surveys.There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.

View Article: PubMed Central - PubMed

Affiliation: OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

ABSTRACT
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8-46.3% and 0-9.1%, respectively. Out of 9 sheep serum samples, 2-4 sera (22.2-44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6-0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.

Show MeSH
Related in: MedlinePlus