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Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice.

Yan Y, Niu L, Deng J, Wang Q, Yu J, Zhang Y, Wang J, Chen J, Wei C, Tan X - J. Vet. Med. Sci. (2014)

Bottom Line: Female BALB/c mice were muscularly inoculated with the plasmids pcAmIL-18, pcDNA3.1 and PBS, respectively.The results showed that pcAmIL-18 remarkably improved the level of specific antibody, IFN-γ and IL-2 in mice sera, the T lymphocyte proliferation index and the percentage of CD4(+) and CD8(+) cells.These data indicated that pcAmIL-18 is a potential adjuvant that promotes specific immunity.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of the Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China.

ABSTRACT
Canine distemper virus (CDV) is a morbillivirus known to cause morbidity and mortality in a broad range of animals. Giant pandas (Ailuropoda melanoleuca), especially captive ones, are susceptible to natural infection with CDV. Interleukin-18 (IL-18) is a powerful adjuvant molecule that can enhance the development of antigen-specific immunity and vaccine efficacy. In this study, a giant panda IL-18 gene eukaryotic expression plasmid (pcAmIL-18) was constructed. Female BALB/c mice were muscularly inoculated with the plasmids pcAmIL-18, pcDNA3.1 and PBS, respectively. They were subsequently injected with an attenuated CDV vaccine for dogs, and the induced humoral and cellular responses were evaluated. The results showed that pcAmIL-18 remarkably improved the level of specific antibody, IFN-γ and IL-2 in mice sera, the T lymphocyte proliferation index and the percentage of CD4(+) and CD8(+) cells. These data indicated that pcAmIL-18 is a potential adjuvant that promotes specific immunity.

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Related in: MedlinePlus

The proliferation of T lymphocytes in the spleens of four mice was analyzed with a CCK-8 kit using ConA as a stimulating agent. The stimulation indices (S.I.) are shown on the vertical axis.
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fig_002: The proliferation of T lymphocytes in the spleens of four mice was analyzed with a CCK-8 kit using ConA as a stimulating agent. The stimulation indices (S.I.) are shown on the vertical axis.

Mentions: Splenocyte proliferation: Splenocyte proliferation was measured for six consecutive weeks from one week after the booster immunization. As shown in Fig. 2Fig. 2.


Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice.

Yan Y, Niu L, Deng J, Wang Q, Yu J, Zhang Y, Wang J, Chen J, Wei C, Tan X - J. Vet. Med. Sci. (2014)

The proliferation of T lymphocytes in the spleens of four mice was analyzed with a CCK-8 kit using ConA as a stimulating agent. The stimulation indices (S.I.) are shown on the vertical axis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363021&req=5

fig_002: The proliferation of T lymphocytes in the spleens of four mice was analyzed with a CCK-8 kit using ConA as a stimulating agent. The stimulation indices (S.I.) are shown on the vertical axis.
Mentions: Splenocyte proliferation: Splenocyte proliferation was measured for six consecutive weeks from one week after the booster immunization. As shown in Fig. 2Fig. 2.

Bottom Line: Female BALB/c mice were muscularly inoculated with the plasmids pcAmIL-18, pcDNA3.1 and PBS, respectively.The results showed that pcAmIL-18 remarkably improved the level of specific antibody, IFN-γ and IL-2 in mice sera, the T lymphocyte proliferation index and the percentage of CD4(+) and CD8(+) cells.These data indicated that pcAmIL-18 is a potential adjuvant that promotes specific immunity.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of the Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China.

ABSTRACT
Canine distemper virus (CDV) is a morbillivirus known to cause morbidity and mortality in a broad range of animals. Giant pandas (Ailuropoda melanoleuca), especially captive ones, are susceptible to natural infection with CDV. Interleukin-18 (IL-18) is a powerful adjuvant molecule that can enhance the development of antigen-specific immunity and vaccine efficacy. In this study, a giant panda IL-18 gene eukaryotic expression plasmid (pcAmIL-18) was constructed. Female BALB/c mice were muscularly inoculated with the plasmids pcAmIL-18, pcDNA3.1 and PBS, respectively. They were subsequently injected with an attenuated CDV vaccine for dogs, and the induced humoral and cellular responses were evaluated. The results showed that pcAmIL-18 remarkably improved the level of specific antibody, IFN-γ and IL-2 in mice sera, the T lymphocyte proliferation index and the percentage of CD4(+) and CD8(+) cells. These data indicated that pcAmIL-18 is a potential adjuvant that promotes specific immunity.

Show MeSH
Related in: MedlinePlus