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Evaluation of the live vaccine efficacy of virulence plasmid-cured, and phoP- or aroA-deficient Salmonella enterica serovar Typhimurium in mice.

Matsui H, Isshiki Y, Eguchi M, Ogawa Y, Shimoji Y - J. Vet. Med. Sci. (2014)

Bottom Line: Typhimurium.Typhimurium strains are promising candidates for safe and effective live S.Typhimurium vaccines.

View Article: PubMed Central - PubMed

Affiliation: Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.

ABSTRACT
We evaluated the protective efficacy of 94-kb virulence plasmid-cured, and phoP- or aroA-deficient strains of Salmonella enterica serovar Typhimurium (ΔphoP or ΔaroA S. Typhimurium) as oral vaccine candidates in BALB/c mice. Two weeks after the completion of 3 oral immunizations with 1 × 10(8) colony-forming units (CFU) of virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium at 10-day intervals, S. Typhimurium lipopolysaccharide (LPS)-specific mucosal secretory immunoglobulin A (s-IgA) antibody titers were detected in the cecal homogenate, bile and lung lavage fluid, but not in the intestinal lavage fluid. In addition, the increases in S. Typhimurium LPS-specific immunoglobulin G (IgG) and IgA antibody titers in the serum were also observed 2 weeks after completing 3 oral immunizations with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. The series of 3 oral immunizations protected the mice against an oral challenge with 5 × 10(8) CFU of the virulent strain of S. Typhimurium, suggesting that both the virulence plasmid-cured, and ΔphoP and ΔaroA S. Typhimurium strains are promising candidates for safe and effective live S. Typhimurium vaccines.

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Efficacy of a single oral immunization with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. (A) The immunization schedule. (B) Anti-S. Typhimurium s-IgA antibody in the intestinal lavage fluid, cecal homogenate, bile and lung lavage fluid in addition to anti-S. Typhimurium IgA and IgG antibodies in serum at weeks 2, 4 and 6 after oral immunization with χ3337phoP (black columns) or UF21 (white columns). The data are combined from two independent experiments (n=10/group).
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fig_001: Efficacy of a single oral immunization with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. (A) The immunization schedule. (B) Anti-S. Typhimurium s-IgA antibody in the intestinal lavage fluid, cecal homogenate, bile and lung lavage fluid in addition to anti-S. Typhimurium IgA and IgG antibodies in serum at weeks 2, 4 and 6 after oral immunization with χ3337phoP (black columns) or UF21 (white columns). The data are combined from two independent experiments (n=10/group).

Mentions: Efficacy of a single oral immunization with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium: Neither χ3337phoP nor UF21 was recovered from the liver, spleen, MLNs or PP at day 5 after oral immunization with 1 × 108 CFU. Unfortunately, we did not detect S. Typhimurium LPS-specific s-IgA antibody in the intestinal lavage fluid, cecal homogenate, bile or lung lavage fluid by ELISA at weeks 2, 4 and 6 after a single oral immunization. However, low levels of S. Typhimurium LPS-specific IgA antibody were detected in the serum at weeks 4 and 6 after a single oral immunization with χ3337phoP (Fig. 1Fig. 1.


Evaluation of the live vaccine efficacy of virulence plasmid-cured, and phoP- or aroA-deficient Salmonella enterica serovar Typhimurium in mice.

Matsui H, Isshiki Y, Eguchi M, Ogawa Y, Shimoji Y - J. Vet. Med. Sci. (2014)

Efficacy of a single oral immunization with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. (A) The immunization schedule. (B) Anti-S. Typhimurium s-IgA antibody in the intestinal lavage fluid, cecal homogenate, bile and lung lavage fluid in addition to anti-S. Typhimurium IgA and IgG antibodies in serum at weeks 2, 4 and 6 after oral immunization with χ3337phoP (black columns) or UF21 (white columns). The data are combined from two independent experiments (n=10/group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363020&req=5

fig_001: Efficacy of a single oral immunization with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. (A) The immunization schedule. (B) Anti-S. Typhimurium s-IgA antibody in the intestinal lavage fluid, cecal homogenate, bile and lung lavage fluid in addition to anti-S. Typhimurium IgA and IgG antibodies in serum at weeks 2, 4 and 6 after oral immunization with χ3337phoP (black columns) or UF21 (white columns). The data are combined from two independent experiments (n=10/group).
Mentions: Efficacy of a single oral immunization with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium: Neither χ3337phoP nor UF21 was recovered from the liver, spleen, MLNs or PP at day 5 after oral immunization with 1 × 108 CFU. Unfortunately, we did not detect S. Typhimurium LPS-specific s-IgA antibody in the intestinal lavage fluid, cecal homogenate, bile or lung lavage fluid by ELISA at weeks 2, 4 and 6 after a single oral immunization. However, low levels of S. Typhimurium LPS-specific IgA antibody were detected in the serum at weeks 4 and 6 after a single oral immunization with χ3337phoP (Fig. 1Fig. 1.

Bottom Line: Typhimurium.Typhimurium strains are promising candidates for safe and effective live S.Typhimurium vaccines.

View Article: PubMed Central - PubMed

Affiliation: Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.

ABSTRACT
We evaluated the protective efficacy of 94-kb virulence plasmid-cured, and phoP- or aroA-deficient strains of Salmonella enterica serovar Typhimurium (ΔphoP or ΔaroA S. Typhimurium) as oral vaccine candidates in BALB/c mice. Two weeks after the completion of 3 oral immunizations with 1 × 10(8) colony-forming units (CFU) of virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium at 10-day intervals, S. Typhimurium lipopolysaccharide (LPS)-specific mucosal secretory immunoglobulin A (s-IgA) antibody titers were detected in the cecal homogenate, bile and lung lavage fluid, but not in the intestinal lavage fluid. In addition, the increases in S. Typhimurium LPS-specific immunoglobulin G (IgG) and IgA antibody titers in the serum were also observed 2 weeks after completing 3 oral immunizations with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. The series of 3 oral immunizations protected the mice against an oral challenge with 5 × 10(8) CFU of the virulent strain of S. Typhimurium, suggesting that both the virulence plasmid-cured, and ΔphoP and ΔaroA S. Typhimurium strains are promising candidates for safe and effective live S. Typhimurium vaccines.

Show MeSH
Related in: MedlinePlus