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Electroacupuncture at the Zusanli and Baihui acupoints ameliorates type-2 diabetes-induced reductions in proliferating cells and differentiated neuroblasts in the hippocampal dentate gyrus with increasing brain-derived neurotrophic factor levels.

Chung JY, Yoo DY, Im W, Choi JH, Yi SS, Youn HY, Hwang IK, Seong JK, Yoon YS - J. Vet. Med. Sci. (2014)

Bottom Line: In contrast, proliferating cells and differentiated neuroblasts were significantly decreased in the ZDF-Sham group compared to the ZLC group.Although EA treatment decreased blood glucose levels, this was not statistically significant when compared to blood glucose levels changes in the ZDF-Sham group.Brain-derived neurotrophic factor (BDNF) levels were significantly decreased in hippocampal homogenates of ZDF-Sham group compared to those in the ZLC group.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
In the current study, we investigated whether electroacupuncture (EA) can inhibit pathological reductions in neurogenesis. Zucker diabetic fatty (ZDF) rats at 7 weeks of age were anesthetized with zoletil, and sham-acupuncture or EA at the Zusanli (ST36) and Baihui (GV20) acupoints was administered once a day for 5 weeks. In the ZDF group that received sham-EA (ZDF-Sham group), the blood glucose level was significantly increased together with age as compared to the control littermates [Zucker lean control (ZLC) rat]. In contrast, proliferating cells and differentiated neuroblasts were significantly decreased in the ZDF-Sham group compared to the ZLC group. Although EA treatment decreased blood glucose levels, this was not statistically significant when compared to blood glucose levels changes in the ZDF-Sham group. However, proliferating cells and differentiated neuroblasts were significantly increased with EA in ZDF rats as compared to those in the ZDF-Sham group. Brain-derived neurotrophic factor (BDNF) levels were significantly decreased in hippocampal homogenates of ZDF-Sham group compared to those in the ZLC group. The EA treatment significantly increased the BDNF levels compared to those in the ZDF-Sham group, and BDNF levels in this group were similar to those in the ZLC group. These results suggest that EA at ST36 and GV20 can ameliorate the reductions in proliferating cells and differentiated neuroblasts in the dentate gyrus induced by type-2 diabetes without significantly reducing blood glucose levels with increasing BDNF levels.

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Immunohistochemistry for Ki67 in the dentate gyrus in ZLC (A), ZDF-Sham (B) and ZDF-EA (C) rats. In the ZLC group, Ki67-positive nuclei (arrows) are detected in the dentate gyrus. In the ZDF-Sham group, fewer Ki67-positive nuclei are observed in the dentate gyrus as compared to those in the ZLC group. In the ZDF-EA group, abundant Ki67-positive nuclei are detected in the dentate gyrus. GCL, granule cell layer; ML, molecular layer; PoL, polymorphic layer. Scale bar=100 µm. D: Quantitative analysis of Ki67-positive nuclei per section in the ZLC, ZDF-Sham and ZDF-EA rats using image analyzer (n=5 per group; *P<0.05, significantly different from the ZLC group, #P<0.05, significantly different from the ZDF-Sham group). Bars indicate means ± SEM.
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fig_002: Immunohistochemistry for Ki67 in the dentate gyrus in ZLC (A), ZDF-Sham (B) and ZDF-EA (C) rats. In the ZLC group, Ki67-positive nuclei (arrows) are detected in the dentate gyrus. In the ZDF-Sham group, fewer Ki67-positive nuclei are observed in the dentate gyrus as compared to those in the ZLC group. In the ZDF-EA group, abundant Ki67-positive nuclei are detected in the dentate gyrus. GCL, granule cell layer; ML, molecular layer; PoL, polymorphic layer. Scale bar=100 µm. D: Quantitative analysis of Ki67-positive nuclei per section in the ZLC, ZDF-Sham and ZDF-EA rats using image analyzer (n=5 per group; *P<0.05, significantly different from the ZLC group, #P<0.05, significantly different from the ZDF-Sham group). Bars indicate means ± SEM.

Mentions: Effects of EA on proliferating cells at the onset of type-2 diabetes: In all groups, Ki67-positive nuclei were mainly detected in the subgranular zone of dentate gyrus. However, the number of Ki67-positive nuclei was significantly different among groups. In the ZDF-Sham group, fewer Ki67-positive nuclei were detected in the subgranular zone of the dentate gyrus than in the ZLC group (Fig. 2A, 2B and 2DFig. 2.


Electroacupuncture at the Zusanli and Baihui acupoints ameliorates type-2 diabetes-induced reductions in proliferating cells and differentiated neuroblasts in the hippocampal dentate gyrus with increasing brain-derived neurotrophic factor levels.

Chung JY, Yoo DY, Im W, Choi JH, Yi SS, Youn HY, Hwang IK, Seong JK, Yoon YS - J. Vet. Med. Sci. (2014)

Immunohistochemistry for Ki67 in the dentate gyrus in ZLC (A), ZDF-Sham (B) and ZDF-EA (C) rats. In the ZLC group, Ki67-positive nuclei (arrows) are detected in the dentate gyrus. In the ZDF-Sham group, fewer Ki67-positive nuclei are observed in the dentate gyrus as compared to those in the ZLC group. In the ZDF-EA group, abundant Ki67-positive nuclei are detected in the dentate gyrus. GCL, granule cell layer; ML, molecular layer; PoL, polymorphic layer. Scale bar=100 µm. D: Quantitative analysis of Ki67-positive nuclei per section in the ZLC, ZDF-Sham and ZDF-EA rats using image analyzer (n=5 per group; *P<0.05, significantly different from the ZLC group, #P<0.05, significantly different from the ZDF-Sham group). Bars indicate means ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4363018&req=5

fig_002: Immunohistochemistry for Ki67 in the dentate gyrus in ZLC (A), ZDF-Sham (B) and ZDF-EA (C) rats. In the ZLC group, Ki67-positive nuclei (arrows) are detected in the dentate gyrus. In the ZDF-Sham group, fewer Ki67-positive nuclei are observed in the dentate gyrus as compared to those in the ZLC group. In the ZDF-EA group, abundant Ki67-positive nuclei are detected in the dentate gyrus. GCL, granule cell layer; ML, molecular layer; PoL, polymorphic layer. Scale bar=100 µm. D: Quantitative analysis of Ki67-positive nuclei per section in the ZLC, ZDF-Sham and ZDF-EA rats using image analyzer (n=5 per group; *P<0.05, significantly different from the ZLC group, #P<0.05, significantly different from the ZDF-Sham group). Bars indicate means ± SEM.
Mentions: Effects of EA on proliferating cells at the onset of type-2 diabetes: In all groups, Ki67-positive nuclei were mainly detected in the subgranular zone of dentate gyrus. However, the number of Ki67-positive nuclei was significantly different among groups. In the ZDF-Sham group, fewer Ki67-positive nuclei were detected in the subgranular zone of the dentate gyrus than in the ZLC group (Fig. 2A, 2B and 2DFig. 2.

Bottom Line: In contrast, proliferating cells and differentiated neuroblasts were significantly decreased in the ZDF-Sham group compared to the ZLC group.Although EA treatment decreased blood glucose levels, this was not statistically significant when compared to blood glucose levels changes in the ZDF-Sham group.Brain-derived neurotrophic factor (BDNF) levels were significantly decreased in hippocampal homogenates of ZDF-Sham group compared to those in the ZLC group.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
In the current study, we investigated whether electroacupuncture (EA) can inhibit pathological reductions in neurogenesis. Zucker diabetic fatty (ZDF) rats at 7 weeks of age were anesthetized with zoletil, and sham-acupuncture or EA at the Zusanli (ST36) and Baihui (GV20) acupoints was administered once a day for 5 weeks. In the ZDF group that received sham-EA (ZDF-Sham group), the blood glucose level was significantly increased together with age as compared to the control littermates [Zucker lean control (ZLC) rat]. In contrast, proliferating cells and differentiated neuroblasts were significantly decreased in the ZDF-Sham group compared to the ZLC group. Although EA treatment decreased blood glucose levels, this was not statistically significant when compared to blood glucose levels changes in the ZDF-Sham group. However, proliferating cells and differentiated neuroblasts were significantly increased with EA in ZDF rats as compared to those in the ZDF-Sham group. Brain-derived neurotrophic factor (BDNF) levels were significantly decreased in hippocampal homogenates of ZDF-Sham group compared to those in the ZLC group. The EA treatment significantly increased the BDNF levels compared to those in the ZDF-Sham group, and BDNF levels in this group were similar to those in the ZLC group. These results suggest that EA at ST36 and GV20 can ameliorate the reductions in proliferating cells and differentiated neuroblasts in the dentate gyrus induced by type-2 diabetes without significantly reducing blood glucose levels with increasing BDNF levels.

Show MeSH
Related in: MedlinePlus