A genome-scale vector resource enables high-throughput reverse genetic screening in a malaria parasite.
Bottom Line: We present a large-scale resource of barcoded vectors with long homology arms for effective modification of the Plasmodium berghei genome.To validate the utility of this resource, we rescreen the P. berghei kinome, using published kinome screens for comparison.We find that several protein kinases function redundantly in asexual blood stages and confirm the targetability of kinases cdpk1, gsk3, tkl3, and PBANKA_082960 by genotyping cloned mutants.
Affiliation: Wellcome Trust Sanger Institute, Hinxton Cambridge CB10 1SA, UK.Show MeSH
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Affiliation: Wellcome Trust Sanger Institute, Hinxton Cambridge CB10 1SA, UK.