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Altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir.

Zinicola M, Lima F, Lima S, Machado V, Gomez M, Döpfer D, Guard C, Bicalho R - PLoS ONE (2015)

Bottom Line: In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi.Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis.Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, United States of America.

ABSTRACT
Bovine digital dermatitis (DD) is the most important infectious disease associated with lameness in cattle worldwide. Since the disease was first described in 1974, a series of Treponema species concurrent with other microbes have been identified in DD lesions, suggesting a polymicrobial etiology. However, the pathogenesis of DD and the source of the causative microbes remain unclear. Here we characterized the microbiomes of healthy skin and skin lesions in dairy cows affected with different stages of DD and investigated the gut microbiome as a potential reservoir for microbes associated with this disease. Discriminant analysis revealed that the microbiomes of healthy skin, active DD lesions (ulcerative and chronic ulcerative) and inactive DD lesions (healing and chronic proliferative) are completely distinct. Treponema denticola, Treponema maltophilum, Treponema medium, Treponema putidum, Treponema phagedenis and Treponema paraluiscuniculi were all found to be present in greater relative abundance in active DD lesions when compared with healthy skin and inactive DD lesions, and these same Treponema species were nearly ubiquitously present in rumen and fecal microbiomes. The relative abundance of Candidatus Amoebophilus asiaticus, a bacterium not previously reported in DD lesions, was increased in both active and inactive lesions when compared with healthy skin. In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi. Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis. Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

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Relative abundance of the major bacterial species associated with digital dermatitis (DD) in healthy skin, inactive DD lesions and active DD lesions.Bacterial types were selected based on the top ranked robust LogWorth of the false discovery rate and average relative abundance of bacterial types in healthy skin, inactive DD lesions, and active DD lesions. Asterisks mean significance. *P < 0.05.
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pone.0120504.g009: Relative abundance of the major bacterial species associated with digital dermatitis (DD) in healthy skin, inactive DD lesions and active DD lesions.Bacterial types were selected based on the top ranked robust LogWorth of the false discovery rate and average relative abundance of bacterial types in healthy skin, inactive DD lesions, and active DD lesions. Asterisks mean significance. *P < 0.05.

Mentions: Microbial types highly associated with active DD lesions were selected based on robust LogWorth of the false discovery rate and relative abundance. The relative abundance of T. denticola, T. maltophilum, T. medium, T. paraluiscuniculi, T. phagedenis and T. putidum were markedly increased in active DD lesions when compared with healthy skin and inactive DD lesions (Fig. 9). Moreover, the proportion of Candidatus Amoebophilus asiaticus was increased in active and inactive DD lesions when compared with healthy skin samples (Fig. 9). Likewise, the consortium of Treponema spp. was increased in lesion stages M2 and M4.1 (active lesions) when compared with either lesion stages M3, M4 (inactive lesions) or healthy skin (S8 Fig.), and Candidatus Amoebophilus asiaticus was increased in lesion stages M2, M4 and M4.1 when compared with lesion stages M1, M3 or healthy skin samples (S8 Fig.).


Altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir.

Zinicola M, Lima F, Lima S, Machado V, Gomez M, Döpfer D, Guard C, Bicalho R - PLoS ONE (2015)

Relative abundance of the major bacterial species associated with digital dermatitis (DD) in healthy skin, inactive DD lesions and active DD lesions.Bacterial types were selected based on the top ranked robust LogWorth of the false discovery rate and average relative abundance of bacterial types in healthy skin, inactive DD lesions, and active DD lesions. Asterisks mean significance. *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362943&req=5

pone.0120504.g009: Relative abundance of the major bacterial species associated with digital dermatitis (DD) in healthy skin, inactive DD lesions and active DD lesions.Bacterial types were selected based on the top ranked robust LogWorth of the false discovery rate and average relative abundance of bacterial types in healthy skin, inactive DD lesions, and active DD lesions. Asterisks mean significance. *P < 0.05.
Mentions: Microbial types highly associated with active DD lesions were selected based on robust LogWorth of the false discovery rate and relative abundance. The relative abundance of T. denticola, T. maltophilum, T. medium, T. paraluiscuniculi, T. phagedenis and T. putidum were markedly increased in active DD lesions when compared with healthy skin and inactive DD lesions (Fig. 9). Moreover, the proportion of Candidatus Amoebophilus asiaticus was increased in active and inactive DD lesions when compared with healthy skin samples (Fig. 9). Likewise, the consortium of Treponema spp. was increased in lesion stages M2 and M4.1 (active lesions) when compared with either lesion stages M3, M4 (inactive lesions) or healthy skin (S8 Fig.), and Candidatus Amoebophilus asiaticus was increased in lesion stages M2, M4 and M4.1 when compared with lesion stages M1, M3 or healthy skin samples (S8 Fig.).

Bottom Line: In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi.Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis.Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, United States of America.

ABSTRACT
Bovine digital dermatitis (DD) is the most important infectious disease associated with lameness in cattle worldwide. Since the disease was first described in 1974, a series of Treponema species concurrent with other microbes have been identified in DD lesions, suggesting a polymicrobial etiology. However, the pathogenesis of DD and the source of the causative microbes remain unclear. Here we characterized the microbiomes of healthy skin and skin lesions in dairy cows affected with different stages of DD and investigated the gut microbiome as a potential reservoir for microbes associated with this disease. Discriminant analysis revealed that the microbiomes of healthy skin, active DD lesions (ulcerative and chronic ulcerative) and inactive DD lesions (healing and chronic proliferative) are completely distinct. Treponema denticola, Treponema maltophilum, Treponema medium, Treponema putidum, Treponema phagedenis and Treponema paraluiscuniculi were all found to be present in greater relative abundance in active DD lesions when compared with healthy skin and inactive DD lesions, and these same Treponema species were nearly ubiquitously present in rumen and fecal microbiomes. The relative abundance of Candidatus Amoebophilus asiaticus, a bacterium not previously reported in DD lesions, was increased in both active and inactive lesions when compared with healthy skin. In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi. Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis. Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

Show MeSH
Related in: MedlinePlus