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Altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir.

Zinicola M, Lima F, Lima S, Machado V, Gomez M, Döpfer D, Guard C, Bicalho R - PLoS ONE (2015)

Bottom Line: In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi.Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis.Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, United States of America.

ABSTRACT
Bovine digital dermatitis (DD) is the most important infectious disease associated with lameness in cattle worldwide. Since the disease was first described in 1974, a series of Treponema species concurrent with other microbes have been identified in DD lesions, suggesting a polymicrobial etiology. However, the pathogenesis of DD and the source of the causative microbes remain unclear. Here we characterized the microbiomes of healthy skin and skin lesions in dairy cows affected with different stages of DD and investigated the gut microbiome as a potential reservoir for microbes associated with this disease. Discriminant analysis revealed that the microbiomes of healthy skin, active DD lesions (ulcerative and chronic ulcerative) and inactive DD lesions (healing and chronic proliferative) are completely distinct. Treponema denticola, Treponema maltophilum, Treponema medium, Treponema putidum, Treponema phagedenis and Treponema paraluiscuniculi were all found to be present in greater relative abundance in active DD lesions when compared with healthy skin and inactive DD lesions, and these same Treponema species were nearly ubiquitously present in rumen and fecal microbiomes. The relative abundance of Candidatus Amoebophilus asiaticus, a bacterium not previously reported in DD lesions, was increased in both active and inactive lesions when compared with healthy skin. In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi. Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis. Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

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Discriminant analysis of superficial and deep strata samples from healthy skin, inactive digital dermatitis lesions and active digital dermatitis lesions.
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pone.0120504.g004: Discriminant analysis of superficial and deep strata samples from healthy skin, inactive digital dermatitis lesions and active digital dermatitis lesions.

Mentions: Healthy skin samples were strongly discriminated from active and inactive DD lesions, and considerable discrimination between active and inactive lesions was also indicated by stepwise discriminant analysis (Fig. 4). Differences in the microbial diversity of superficial and deep strata samples between healthy skin and DD lesions (active and inactive) are illustrated by Canonical 1 (Fig. 4). Differences between active and inactive lesions are illustrated by Canonical 2 (Fig. 4). Canonical 3 illustrates differences, mostly minor, between superficial and deep samples of inactive lesions (Fig. 4). The canonical scores for each bacterial taxon that were used to discriminate the microbiomes of healthy skin, active DD lesions, and inactive lesions of superficial and deep samples are presented in S1, S2 and S3 Figs., respectively.


Altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir.

Zinicola M, Lima F, Lima S, Machado V, Gomez M, Döpfer D, Guard C, Bicalho R - PLoS ONE (2015)

Discriminant analysis of superficial and deep strata samples from healthy skin, inactive digital dermatitis lesions and active digital dermatitis lesions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362943&req=5

pone.0120504.g004: Discriminant analysis of superficial and deep strata samples from healthy skin, inactive digital dermatitis lesions and active digital dermatitis lesions.
Mentions: Healthy skin samples were strongly discriminated from active and inactive DD lesions, and considerable discrimination between active and inactive lesions was also indicated by stepwise discriminant analysis (Fig. 4). Differences in the microbial diversity of superficial and deep strata samples between healthy skin and DD lesions (active and inactive) are illustrated by Canonical 1 (Fig. 4). Differences between active and inactive lesions are illustrated by Canonical 2 (Fig. 4). Canonical 3 illustrates differences, mostly minor, between superficial and deep samples of inactive lesions (Fig. 4). The canonical scores for each bacterial taxon that were used to discriminate the microbiomes of healthy skin, active DD lesions, and inactive lesions of superficial and deep samples are presented in S1, S2 and S3 Figs., respectively.

Bottom Line: In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi.Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis.Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, United States of America.

ABSTRACT
Bovine digital dermatitis (DD) is the most important infectious disease associated with lameness in cattle worldwide. Since the disease was first described in 1974, a series of Treponema species concurrent with other microbes have been identified in DD lesions, suggesting a polymicrobial etiology. However, the pathogenesis of DD and the source of the causative microbes remain unclear. Here we characterized the microbiomes of healthy skin and skin lesions in dairy cows affected with different stages of DD and investigated the gut microbiome as a potential reservoir for microbes associated with this disease. Discriminant analysis revealed that the microbiomes of healthy skin, active DD lesions (ulcerative and chronic ulcerative) and inactive DD lesions (healing and chronic proliferative) are completely distinct. Treponema denticola, Treponema maltophilum, Treponema medium, Treponema putidum, Treponema phagedenis and Treponema paraluiscuniculi were all found to be present in greater relative abundance in active DD lesions when compared with healthy skin and inactive DD lesions, and these same Treponema species were nearly ubiquitously present in rumen and fecal microbiomes. The relative abundance of Candidatus Amoebophilus asiaticus, a bacterium not previously reported in DD lesions, was increased in both active and inactive lesions when compared with healthy skin. In conclusion, our data support the concept that DD is a polymicrobial disease, with active DD lesions having a markedly distinct microbiome dominated by T. denticola, T. maltophilum, T. medium, T. putidum, T. phagedenis and T. paraluiscuniculi. Furthermore, these Treponema species are nearly ubiquitously found in rumen and fecal microbiomes, suggesting that the gut is an important reservoir of microbes involved in DD pathogenesis. Additionally, the bacterium Candidatus Amoebophilus asiaticus was highly abundant in active and inactive DD lesions.

Show MeSH
Related in: MedlinePlus