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Overexpression of periostin in stroma positively associated with aggressive prostate cancer.

Tian Y, Choi CH, Li QK, Rahmatpanah FB, Chen X, Kim SR, Veltri R, Chia D, Zhang Z, Mercola D, Zhang H - PLoS ONE (2015)

Bottom Line: The TMAs were further scored by pathologists to characterize the stromal staining and epithelial staining.The periostin staining was observed mainly in peritumoral stromal cells and in some cases in tumor epithelial cells though the stronger staining was found in peritumoral stromal cells.The results showed that periostin staining was positively correlated with increasing Gleason score and the aggressiveness of prostate disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Johns Hopkins University, Baltimore, Maryland, United States of America.

ABSTRACT

Background: Periostin is an important extracellular matrix protein involved in cell development and adhesion. Previously, we identified periostin to be up-regulated in aggressive prostate cancer (CaP) using quantitative glycoproteomics and mass spectrometry. The expression of periostin was further evaluated in primary radical prostatectomy (RP) prostate tumors and adjacent non-tumorous prostate tissues using immunohistochemistry (IHC). Our IHC results revealed a low background periostin levels in the adjacent non-tumorous prostate tissues, but overexpressed periostin levels in the peritumoral stroma of primary CaP tumors.

Methods: In this study, periostin expression in CaP was further examined on multiple tissue microarrays (TMAs), which were conducted in four laboratories. To achieve consistent staining, all TMAs were stained with same protocol and scored by same image computation tool to determine the total periostin staining intensities. The TMAs were further scored by pathologists to characterize the stromal staining and epithelial staining.

Results: The periostin staining was observed mainly in peritumoral stromal cells and in some cases in tumor epithelial cells though the stronger staining was found in peritumoral stromal cells. Both periostin stromal staining and epithelial staining can differentiate BPH from CaP including low grade CaP (Gleason score ≤6), with significant p-value of 2.2e-16 and 0.001, respectively. Periostin epithelial staining differentiated PIN from low grade CaP (Gleason score ≤6) (p=0.001), while periostin stromal staining differentiated low grade Cap (Gleason score ≤6) from high grade Cap (Gleason score ≤6) (p=1.7e-05). In addition, a positive correlation between total periostin staining and Gleason score was observed (r=0.87, p=0.002).

Conclusions: The results showed that periostin staining was positively correlated with increasing Gleason score and the aggressiveness of prostate disease.

No MeSH data available.


Related in: MedlinePlus

Correlation analysis of the positivity of total periostin staining and Gleason score.(A) Box plots of the positivity of periostin for the three TMAs from UCLA, (B) Box plots of the positivity of periostin for the three TMAs from UCI, (C) the positive correlation of fold changes and Gleason score using all nine TMAs. Percentage of staining (positivity) was calculated as total number of positive pixels divided by total number of pixels.
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pone.0121502.g003: Correlation analysis of the positivity of total periostin staining and Gleason score.(A) Box plots of the positivity of periostin for the three TMAs from UCLA, (B) Box plots of the positivity of periostin for the three TMAs from UCI, (C) the positive correlation of fold changes and Gleason score using all nine TMAs. Percentage of staining (positivity) was calculated as total number of positive pixels divided by total number of pixels.

Mentions: To determine the correlation of periostin expression and Gleason score, all nine TMAs were analyzed using the same image computational tool. Percentage of staining was calculated as total number of positive pixels divided by total number of pixels. The increases of periostin total staining intensity were found in the prostate tumors with increased Gleason score in the three TMAs from JHU (Fig. 1B), the three TMAs from UCLA (Fig. 3A), and the three TMAs from UCI (Fig. 3B). The results show that a positive correlation between total periostin staining and Gleason score (r = 0.87, p = 0.002) (Fig. 3C).


Overexpression of periostin in stroma positively associated with aggressive prostate cancer.

Tian Y, Choi CH, Li QK, Rahmatpanah FB, Chen X, Kim SR, Veltri R, Chia D, Zhang Z, Mercola D, Zhang H - PLoS ONE (2015)

Correlation analysis of the positivity of total periostin staining and Gleason score.(A) Box plots of the positivity of periostin for the three TMAs from UCLA, (B) Box plots of the positivity of periostin for the three TMAs from UCI, (C) the positive correlation of fold changes and Gleason score using all nine TMAs. Percentage of staining (positivity) was calculated as total number of positive pixels divided by total number of pixels.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362940&req=5

pone.0121502.g003: Correlation analysis of the positivity of total periostin staining and Gleason score.(A) Box plots of the positivity of periostin for the three TMAs from UCLA, (B) Box plots of the positivity of periostin for the three TMAs from UCI, (C) the positive correlation of fold changes and Gleason score using all nine TMAs. Percentage of staining (positivity) was calculated as total number of positive pixels divided by total number of pixels.
Mentions: To determine the correlation of periostin expression and Gleason score, all nine TMAs were analyzed using the same image computational tool. Percentage of staining was calculated as total number of positive pixels divided by total number of pixels. The increases of periostin total staining intensity were found in the prostate tumors with increased Gleason score in the three TMAs from JHU (Fig. 1B), the three TMAs from UCLA (Fig. 3A), and the three TMAs from UCI (Fig. 3B). The results show that a positive correlation between total periostin staining and Gleason score (r = 0.87, p = 0.002) (Fig. 3C).

Bottom Line: The TMAs were further scored by pathologists to characterize the stromal staining and epithelial staining.The periostin staining was observed mainly in peritumoral stromal cells and in some cases in tumor epithelial cells though the stronger staining was found in peritumoral stromal cells.The results showed that periostin staining was positively correlated with increasing Gleason score and the aggressiveness of prostate disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Johns Hopkins University, Baltimore, Maryland, United States of America.

ABSTRACT

Background: Periostin is an important extracellular matrix protein involved in cell development and adhesion. Previously, we identified periostin to be up-regulated in aggressive prostate cancer (CaP) using quantitative glycoproteomics and mass spectrometry. The expression of periostin was further evaluated in primary radical prostatectomy (RP) prostate tumors and adjacent non-tumorous prostate tissues using immunohistochemistry (IHC). Our IHC results revealed a low background periostin levels in the adjacent non-tumorous prostate tissues, but overexpressed periostin levels in the peritumoral stroma of primary CaP tumors.

Methods: In this study, periostin expression in CaP was further examined on multiple tissue microarrays (TMAs), which were conducted in four laboratories. To achieve consistent staining, all TMAs were stained with same protocol and scored by same image computation tool to determine the total periostin staining intensities. The TMAs were further scored by pathologists to characterize the stromal staining and epithelial staining.

Results: The periostin staining was observed mainly in peritumoral stromal cells and in some cases in tumor epithelial cells though the stronger staining was found in peritumoral stromal cells. Both periostin stromal staining and epithelial staining can differentiate BPH from CaP including low grade CaP (Gleason score ≤6), with significant p-value of 2.2e-16 and 0.001, respectively. Periostin epithelial staining differentiated PIN from low grade CaP (Gleason score ≤6) (p=0.001), while periostin stromal staining differentiated low grade Cap (Gleason score ≤6) from high grade Cap (Gleason score ≤6) (p=1.7e-05). In addition, a positive correlation between total periostin staining and Gleason score was observed (r=0.87, p=0.002).

Conclusions: The results showed that periostin staining was positively correlated with increasing Gleason score and the aggressiveness of prostate disease.

No MeSH data available.


Related in: MedlinePlus