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2H,3H-decafluoropentane-based nanodroplets: new perspectives for oxygen delivery to hypoxic cutaneous tissues.

Prato M, Magnetto C, Jose J, Khadjavi A, Cavallo F, Quaglino E, Panariti A, Rivolta I, Benintende E, Varetto G, Argenziano M, Troia A, Cavalli R, Guiot C - PLoS ONE (2015)

Bottom Line: In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging.Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs.Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Neuroscienze, Università di Torino, Torino, Italy.

ABSTRACT
Perfluoropentane (PFP)-based oxygen-loaded nanobubbles (OLNBs) were previously proposed as adjuvant therapeutic tools for pathologies of different etiology sharing hypoxia as a common feature, including cancer, infection, and autoimmunity. Here we introduce a new platform of oxygen nanocarriers, based on 2H,3H-decafluoropentane (DFP) as core fluorocarbon. These new nanocarriers have been named oxygen-loaded nanodroplets (OLNDs) since DFP is liquid at body temperature, unlike gaseous PFP. Dextran-shelled OLNDs, available either in liquid or gel formulations, display spherical morphology, ~600 nm diameters, anionic charge, good oxygen carrying capacity, and no toxic effects on human keratinocytes after cell internalization. In vitro OLNDs result more effective in releasing oxygen to hypoxic environments than former OLNBs, as demonstrated by analysis through oxymetry. In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging. Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs. Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

No MeSH data available.


Related in: MedlinePlus

Topical treatment with US-activated OLNDs effectively enhances tcpO2in vivo.The shaved abdomens of eight anesthetized mice were topically treated with OLND gel formulation and sonicated (f = 1 MHz; P = 5 W; t = 30 sec). Before and after treatment, tcpO2 was monitored through transcutaneous oxymetry. Panel A. Short-term time-course (0–15 min) tcpO2 monitoring of three mice before and after treatment with OLND gel formulation. Data are shown as means ± SD. Results were also analyzed for statistical significance by Student’s t test. Versus untreated mice: p < 0.01. B. Long-term end-point (1 h) tcpO2 measurement of five mice (m1-m5) before and after treatment with OLND gel formulation. Data are shown individually per each mouse.
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pone.0119769.g007: Topical treatment with US-activated OLNDs effectively enhances tcpO2in vivo.The shaved abdomens of eight anesthetized mice were topically treated with OLND gel formulation and sonicated (f = 1 MHz; P = 5 W; t = 30 sec). Before and after treatment, tcpO2 was monitored through transcutaneous oxymetry. Panel A. Short-term time-course (0–15 min) tcpO2 monitoring of three mice before and after treatment with OLND gel formulation. Data are shown as means ± SD. Results were also analyzed for statistical significance by Student’s t test. Versus untreated mice: p < 0.01. B. Long-term end-point (1 h) tcpO2 measurement of five mice (m1-m5) before and after treatment with OLND gel formulation. Data are shown individually per each mouse.

Mentions: Thereafter, OLND ability to improve tissue oxygenation in vivo upon US treatment was investigated. The shaved abdomens of eight anesthetized mice were topically treated with OLNDs and sonicated for 30 sec (f = 1 MHz; P = 5 W). Skin oxygenation was investigated through transcutaneous oxymetry (Fig. 7: panel A, 0–15 min; panel B, 1 h) before and after the treatment. Basal tcpO2 values in mice were inhomogeneous, possibly as a consequence of the different level of peripheral vasoconstriction induced by anesthesia. Nevertheless, after topical administration of US-activated OLNDs hypoxic mice displayed larger oxygenation levels in a time-sustained manner up to 1 h.


2H,3H-decafluoropentane-based nanodroplets: new perspectives for oxygen delivery to hypoxic cutaneous tissues.

Prato M, Magnetto C, Jose J, Khadjavi A, Cavallo F, Quaglino E, Panariti A, Rivolta I, Benintende E, Varetto G, Argenziano M, Troia A, Cavalli R, Guiot C - PLoS ONE (2015)

Topical treatment with US-activated OLNDs effectively enhances tcpO2in vivo.The shaved abdomens of eight anesthetized mice were topically treated with OLND gel formulation and sonicated (f = 1 MHz; P = 5 W; t = 30 sec). Before and after treatment, tcpO2 was monitored through transcutaneous oxymetry. Panel A. Short-term time-course (0–15 min) tcpO2 monitoring of three mice before and after treatment with OLND gel formulation. Data are shown as means ± SD. Results were also analyzed for statistical significance by Student’s t test. Versus untreated mice: p < 0.01. B. Long-term end-point (1 h) tcpO2 measurement of five mice (m1-m5) before and after treatment with OLND gel formulation. Data are shown individually per each mouse.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362938&req=5

pone.0119769.g007: Topical treatment with US-activated OLNDs effectively enhances tcpO2in vivo.The shaved abdomens of eight anesthetized mice were topically treated with OLND gel formulation and sonicated (f = 1 MHz; P = 5 W; t = 30 sec). Before and after treatment, tcpO2 was monitored through transcutaneous oxymetry. Panel A. Short-term time-course (0–15 min) tcpO2 monitoring of three mice before and after treatment with OLND gel formulation. Data are shown as means ± SD. Results were also analyzed for statistical significance by Student’s t test. Versus untreated mice: p < 0.01. B. Long-term end-point (1 h) tcpO2 measurement of five mice (m1-m5) before and after treatment with OLND gel formulation. Data are shown individually per each mouse.
Mentions: Thereafter, OLND ability to improve tissue oxygenation in vivo upon US treatment was investigated. The shaved abdomens of eight anesthetized mice were topically treated with OLNDs and sonicated for 30 sec (f = 1 MHz; P = 5 W). Skin oxygenation was investigated through transcutaneous oxymetry (Fig. 7: panel A, 0–15 min; panel B, 1 h) before and after the treatment. Basal tcpO2 values in mice were inhomogeneous, possibly as a consequence of the different level of peripheral vasoconstriction induced by anesthesia. Nevertheless, after topical administration of US-activated OLNDs hypoxic mice displayed larger oxygenation levels in a time-sustained manner up to 1 h.

Bottom Line: In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging.Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs.Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Neuroscienze, Università di Torino, Torino, Italy.

ABSTRACT
Perfluoropentane (PFP)-based oxygen-loaded nanobubbles (OLNBs) were previously proposed as adjuvant therapeutic tools for pathologies of different etiology sharing hypoxia as a common feature, including cancer, infection, and autoimmunity. Here we introduce a new platform of oxygen nanocarriers, based on 2H,3H-decafluoropentane (DFP) as core fluorocarbon. These new nanocarriers have been named oxygen-loaded nanodroplets (OLNDs) since DFP is liquid at body temperature, unlike gaseous PFP. Dextran-shelled OLNDs, available either in liquid or gel formulations, display spherical morphology, ~600 nm diameters, anionic charge, good oxygen carrying capacity, and no toxic effects on human keratinocytes after cell internalization. In vitro OLNDs result more effective in releasing oxygen to hypoxic environments than former OLNBs, as demonstrated by analysis through oxymetry. In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging. Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs. Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

No MeSH data available.


Related in: MedlinePlus