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2H,3H-decafluoropentane-based nanodroplets: new perspectives for oxygen delivery to hypoxic cutaneous tissues.

Prato M, Magnetto C, Jose J, Khadjavi A, Cavallo F, Quaglino E, Panariti A, Rivolta I, Benintende E, Varetto G, Argenziano M, Troia A, Cavalli R, Guiot C - PLoS ONE (2015)

Bottom Line: In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging.Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs.Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Neuroscienze, Università di Torino, Torino, Italy.

ABSTRACT
Perfluoropentane (PFP)-based oxygen-loaded nanobubbles (OLNBs) were previously proposed as adjuvant therapeutic tools for pathologies of different etiology sharing hypoxia as a common feature, including cancer, infection, and autoimmunity. Here we introduce a new platform of oxygen nanocarriers, based on 2H,3H-decafluoropentane (DFP) as core fluorocarbon. These new nanocarriers have been named oxygen-loaded nanodroplets (OLNDs) since DFP is liquid at body temperature, unlike gaseous PFP. Dextran-shelled OLNDs, available either in liquid or gel formulations, display spherical morphology, ~600 nm diameters, anionic charge, good oxygen carrying capacity, and no toxic effects on human keratinocytes after cell internalization. In vitro OLNDs result more effective in releasing oxygen to hypoxic environments than former OLNBs, as demonstrated by analysis through oxymetry. In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging. Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs. Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

No MeSH data available.


Related in: MedlinePlus

OLND are not cytotoxic and improve viability of human keratinocytes in vitro.Human keratinocytes (106 cells/2 ml Panserin 601 medium) were left untreated or treated with different doses (100–400 l) of OLND PBS formulation for 24 h in normoxia (20% O2; white-squared curves, both panels) or hypoxia (1% O2; black-squared curves, both panels). Thereafter, OLND cytotoxicity (Panel A) was measured through LDH assay, whereas cell viability (Panel B) was measured through MTT assay. Results are shown as means ± SEM from three independent experiments. Data were also evaluated for significance by ANOVA. Panel A. Versus normoxic untreated cells: p not significant. Panel B. Versus normoxic untreated cells: * p < 0.01; ** p < 0.001; *** p < 0.0001.
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pone.0119769.g004: OLND are not cytotoxic and improve viability of human keratinocytes in vitro.Human keratinocytes (106 cells/2 ml Panserin 601 medium) were left untreated or treated with different doses (100–400 l) of OLND PBS formulation for 24 h in normoxia (20% O2; white-squared curves, both panels) or hypoxia (1% O2; black-squared curves, both panels). Thereafter, OLND cytotoxicity (Panel A) was measured through LDH assay, whereas cell viability (Panel B) was measured through MTT assay. Results are shown as means ± SEM from three independent experiments. Data were also evaluated for significance by ANOVA. Panel A. Versus normoxic untreated cells: p not significant. Panel B. Versus normoxic untreated cells: * p < 0.01; ** p < 0.001; *** p < 0.0001.

Mentions: Thereafter, OLND toxicity and cell viability both in normoxic (20% O2) and hypoxic (1% O2) conditions were evaluated by LDH and MTT assays, respectively. As shown in Fig. 4A, increasing volumes of OLND PBS suspensions, ranging from 100 to 400 μl, did not result toxic to cells either in normoxia or hypoxia. Eventually, OLNDs improved keratinocyte viability in either condition of oxygenation (Fig. 4B).


2H,3H-decafluoropentane-based nanodroplets: new perspectives for oxygen delivery to hypoxic cutaneous tissues.

Prato M, Magnetto C, Jose J, Khadjavi A, Cavallo F, Quaglino E, Panariti A, Rivolta I, Benintende E, Varetto G, Argenziano M, Troia A, Cavalli R, Guiot C - PLoS ONE (2015)

OLND are not cytotoxic and improve viability of human keratinocytes in vitro.Human keratinocytes (106 cells/2 ml Panserin 601 medium) were left untreated or treated with different doses (100–400 l) of OLND PBS formulation for 24 h in normoxia (20% O2; white-squared curves, both panels) or hypoxia (1% O2; black-squared curves, both panels). Thereafter, OLND cytotoxicity (Panel A) was measured through LDH assay, whereas cell viability (Panel B) was measured through MTT assay. Results are shown as means ± SEM from three independent experiments. Data were also evaluated for significance by ANOVA. Panel A. Versus normoxic untreated cells: p not significant. Panel B. Versus normoxic untreated cells: * p < 0.01; ** p < 0.001; *** p < 0.0001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362938&req=5

pone.0119769.g004: OLND are not cytotoxic and improve viability of human keratinocytes in vitro.Human keratinocytes (106 cells/2 ml Panserin 601 medium) were left untreated or treated with different doses (100–400 l) of OLND PBS formulation for 24 h in normoxia (20% O2; white-squared curves, both panels) or hypoxia (1% O2; black-squared curves, both panels). Thereafter, OLND cytotoxicity (Panel A) was measured through LDH assay, whereas cell viability (Panel B) was measured through MTT assay. Results are shown as means ± SEM from three independent experiments. Data were also evaluated for significance by ANOVA. Panel A. Versus normoxic untreated cells: p not significant. Panel B. Versus normoxic untreated cells: * p < 0.01; ** p < 0.001; *** p < 0.0001.
Mentions: Thereafter, OLND toxicity and cell viability both in normoxic (20% O2) and hypoxic (1% O2) conditions were evaluated by LDH and MTT assays, respectively. As shown in Fig. 4A, increasing volumes of OLND PBS suspensions, ranging from 100 to 400 μl, did not result toxic to cells either in normoxia or hypoxia. Eventually, OLNDs improved keratinocyte viability in either condition of oxygenation (Fig. 4B).

Bottom Line: In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging.Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs.Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Neuroscienze, Università di Torino, Torino, Italy.

ABSTRACT
Perfluoropentane (PFP)-based oxygen-loaded nanobubbles (OLNBs) were previously proposed as adjuvant therapeutic tools for pathologies of different etiology sharing hypoxia as a common feature, including cancer, infection, and autoimmunity. Here we introduce a new platform of oxygen nanocarriers, based on 2H,3H-decafluoropentane (DFP) as core fluorocarbon. These new nanocarriers have been named oxygen-loaded nanodroplets (OLNDs) since DFP is liquid at body temperature, unlike gaseous PFP. Dextran-shelled OLNDs, available either in liquid or gel formulations, display spherical morphology, ~600 nm diameters, anionic charge, good oxygen carrying capacity, and no toxic effects on human keratinocytes after cell internalization. In vitro OLNDs result more effective in releasing oxygen to hypoxic environments than former OLNBs, as demonstrated by analysis through oxymetry. In vivo, OLNDs effectively enhance oxy-hemoglobin levels, as emerged from investigation by photoacoustic imaging. Interestingly, ultrasound (US) treatment further improves transdermal oxygen release from OLNDs. Taken together, these data suggest that US-activated, DFP-based OLNDs might be innovative, suitable and cost-effective devices to topically treat hypoxia-associated pathologies of the cutaneous tissues.

No MeSH data available.


Related in: MedlinePlus