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Anticoccidial and antioxidant activities of zinc oxide nanoparticles on Eimeria papillata-induced infection in the jejunum.

Dkhil MA, Al-Quraishy S, Wahab R - Int J Nanomedicine (2015)

Bottom Line: This output was significantly decreased, to 12.5×10(3)±1,000 oocysts, in mice treated with ZNPs.This was evidenced (1) through an increase in the inflammatory histological score, (2) through increased production of nitric oxide and malondialdehyde, and (3) through a decrease in both the glutathione level and goblet cell number in mice jejuna.Our results indicate, therefore, that ZNPs have protective effects against E. papillata-induced coccidiosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia ; Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.

ABSTRACT
Nanomedicine has recently emerged as a better option for the treatment of various diseases. Here, we investigated the in vivo anticoccidial properties of zinc oxide nanoparticles (ZNPs). ZNPs were crystalline in nature, with a smooth and spherical surface and a diameter in the range of ~10-15 nm. The X-ray diffraction pattern was utilized to identify the crystalline property of the grown ZNPs, whereas field emission scanning electron microscopy was employed to check the size and morphology of the ZNPs. The data showed that mice infected with Eimeria papillata produced 29.7×10(3)±1,500 oocysts/g feces on day 5 postinfection. This output was significantly decreased, to 12.5×10(3)±1,000 oocysts, in mice treated with ZNPs. Infection also induced inflammation and injury of the jejunum. This was evidenced (1) through an increase in the inflammatory histological score, (2) through increased production of nitric oxide and malondialdehyde, and (3) through a decrease in both the glutathione level and goblet cell number in mice jejuna. All these infection-induced parameters were significantly altered during treatment with ZNPs. Our results indicate, therefore, that ZNPs have protective effects against E. papillata-induced coccidiosis.

No MeSH data available.


Related in: MedlinePlus

ZNP induced changes in the number of goblet cells in the jejunum of noninfected mice and mice infected with Eimeria papillata on day 5 postinnoculation.Notes: Data were obtained from Alcian blue-stained sections. Values are expressed as mean ± SD. aSignificant in respect to the noninfected (− ZNP) group (P≤0.05); bsignificant in respect to the infected (− ZNP) group (P≤0.05).Abbreviations: SD, standard deviation; CVU, crypt-villus unit; ZNP, zinc oxide nanoparticle.
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f5-ijn-10-1961: ZNP induced changes in the number of goblet cells in the jejunum of noninfected mice and mice infected with Eimeria papillata on day 5 postinnoculation.Notes: Data were obtained from Alcian blue-stained sections. Values are expressed as mean ± SD. aSignificant in respect to the noninfected (− ZNP) group (P≤0.05); bsignificant in respect to the infected (− ZNP) group (P≤0.05).Abbreviations: SD, standard deviation; CVU, crypt-villus unit; ZNP, zinc oxide nanoparticle.

Mentions: Alcian blue-stained sections showed goblet cells as blue-stained (Figure S1). On day 5 PI with E. papillata, the number of goblet cells was significantly decreased when compared with the control noninfected mice (Figure 5). ZNPs, however, were associated with the restoration of the number of goblet cells (Figure 5).


Anticoccidial and antioxidant activities of zinc oxide nanoparticles on Eimeria papillata-induced infection in the jejunum.

Dkhil MA, Al-Quraishy S, Wahab R - Int J Nanomedicine (2015)

ZNP induced changes in the number of goblet cells in the jejunum of noninfected mice and mice infected with Eimeria papillata on day 5 postinnoculation.Notes: Data were obtained from Alcian blue-stained sections. Values are expressed as mean ± SD. aSignificant in respect to the noninfected (− ZNP) group (P≤0.05); bsignificant in respect to the infected (− ZNP) group (P≤0.05).Abbreviations: SD, standard deviation; CVU, crypt-villus unit; ZNP, zinc oxide nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362905&req=5

f5-ijn-10-1961: ZNP induced changes in the number of goblet cells in the jejunum of noninfected mice and mice infected with Eimeria papillata on day 5 postinnoculation.Notes: Data were obtained from Alcian blue-stained sections. Values are expressed as mean ± SD. aSignificant in respect to the noninfected (− ZNP) group (P≤0.05); bsignificant in respect to the infected (− ZNP) group (P≤0.05).Abbreviations: SD, standard deviation; CVU, crypt-villus unit; ZNP, zinc oxide nanoparticle.
Mentions: Alcian blue-stained sections showed goblet cells as blue-stained (Figure S1). On day 5 PI with E. papillata, the number of goblet cells was significantly decreased when compared with the control noninfected mice (Figure 5). ZNPs, however, were associated with the restoration of the number of goblet cells (Figure 5).

Bottom Line: This output was significantly decreased, to 12.5×10(3)±1,000 oocysts, in mice treated with ZNPs.This was evidenced (1) through an increase in the inflammatory histological score, (2) through increased production of nitric oxide and malondialdehyde, and (3) through a decrease in both the glutathione level and goblet cell number in mice jejuna.Our results indicate, therefore, that ZNPs have protective effects against E. papillata-induced coccidiosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia ; Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.

ABSTRACT
Nanomedicine has recently emerged as a better option for the treatment of various diseases. Here, we investigated the in vivo anticoccidial properties of zinc oxide nanoparticles (ZNPs). ZNPs were crystalline in nature, with a smooth and spherical surface and a diameter in the range of ~10-15 nm. The X-ray diffraction pattern was utilized to identify the crystalline property of the grown ZNPs, whereas field emission scanning electron microscopy was employed to check the size and morphology of the ZNPs. The data showed that mice infected with Eimeria papillata produced 29.7×10(3)±1,500 oocysts/g feces on day 5 postinfection. This output was significantly decreased, to 12.5×10(3)±1,000 oocysts, in mice treated with ZNPs. Infection also induced inflammation and injury of the jejunum. This was evidenced (1) through an increase in the inflammatory histological score, (2) through increased production of nitric oxide and malondialdehyde, and (3) through a decrease in both the glutathione level and goblet cell number in mice jejuna. All these infection-induced parameters were significantly altered during treatment with ZNPs. Our results indicate, therefore, that ZNPs have protective effects against E. papillata-induced coccidiosis.

No MeSH data available.


Related in: MedlinePlus