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Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects.

Aravinthan A, Govarthanan M, Selvam K, Praburaman L, Selvankumar T, Balamurugan R, Kamala-Kannan S, Kim JH - Int J Nanomedicine (2015)

Bottom Line: The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens.In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes.The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea.

ABSTRACT
A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.

No MeSH data available.


Related in: MedlinePlus

TEM image of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles.
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f2-ijn-10-1977: TEM image of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles.

Mentions: The morphology and size of the phytosynthesized AgNPs were observed by TEM images (Figure 2). The particles were spherical in shape, monodisperse, and the size of the particles varied from 10–70 nm. To confirm the presence of Ag, the samples were analyzed in SEM–EDS, and the results are shown in Figure 3. The results showed strong silver signals (3 keV), along with weak oxygen and carbon peaks, which might have originated from the tuber extract. The results are consistent with previous studies that reported the strong peak for AgNPs at 3 keV.7,8 The EDS quantitative analysis showed the presence of silver (100%) without any contaminants.


Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects.

Aravinthan A, Govarthanan M, Selvam K, Praburaman L, Selvankumar T, Balamurugan R, Kamala-Kannan S, Kim JH - Int J Nanomedicine (2015)

TEM image of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362901&req=5

f2-ijn-10-1977: TEM image of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles.
Mentions: The morphology and size of the phytosynthesized AgNPs were observed by TEM images (Figure 2). The particles were spherical in shape, monodisperse, and the size of the particles varied from 10–70 nm. To confirm the presence of Ag, the samples were analyzed in SEM–EDS, and the results are shown in Figure 3. The results showed strong silver signals (3 keV), along with weak oxygen and carbon peaks, which might have originated from the tuber extract. The results are consistent with previous studies that reported the strong peak for AgNPs at 3 keV.7,8 The EDS quantitative analysis showed the presence of silver (100%) without any contaminants.

Bottom Line: The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens.In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes.The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea.

ABSTRACT
A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.

No MeSH data available.


Related in: MedlinePlus